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Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells

  • Authors:
    • Yaqiong Yu
    • Di Yang
    • Lihong Qiu
    • Hirohiko Okamura
    • Jiajie Guo
    • Tatsuji Haneji
  • View Affiliations / Copyright

    Affiliations: Department of Endodontics, School of Stomatology, China Medical University, Shenyang, Liaoning 110002, P.R. China, Department of Histology and Oral Histology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima 770-8504, Japan
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1371-1376
    |
    Published online on: June 25, 2014
       https://doi.org/10.3892/mmr.2014.2353
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Abstract

Osteoblasts produce various types of cytokines under pathological conditions and control osteoclast differentiation. Tumor necrosis factor-α (TNF-α) has been demonstrated to exert complex effects in osteoblasts under local inflammatory conditions, including in periodontal and periapical diseases. Interleukin-34 (IL-34) has been recently identified as a novel regulatory factor for the differentiation and function of osteoclasts. The present study provides the first evidence, to the best of our knowledge, that the expression of IL-34 is induced by TNF-α through nuclear factor-κB (NF-κB) activation in MC3T3-E1 osteoblastic cells. TNF-α induced IL-34 expression in a dose- and time-dependent manner. Immunocytochemistry with an NF-κB antibody demonstrated that NF-κB was mainly localized in the cytoplasm of the untreated MC3T3-E1 cells. Rapid translocation of NF-κB from the cytoplasm to the nucleus was observed in the cells treated with TNF-α for 15 min. Translocation and transcriptional activity of NF-κB were also determined by western blotting and a luciferase reporter assay, respectively. Pretreatment with 100 µM CAPE, an inhibitor of NF-κB, significantly inhibited TNF-α-induced IL-34 expression. These results indicate that TNF-α induces IL-34 expression via NF-κB in osteoblasts.
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Copy and paste a formatted citation
Spandidos Publications style
Yu Y, Yang D, Qiu L, Okamura H, Guo J and Haneji T: Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells. Mol Med Rep 10: 1371-1376, 2014.
APA
Yu, Y., Yang, D., Qiu, L., Okamura, H., Guo, J., & Haneji, T. (2014). Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells. Molecular Medicine Reports, 10, 1371-1376. https://doi.org/10.3892/mmr.2014.2353
MLA
Yu, Y., Yang, D., Qiu, L., Okamura, H., Guo, J., Haneji, T."Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells". Molecular Medicine Reports 10.3 (2014): 1371-1376.
Chicago
Yu, Y., Yang, D., Qiu, L., Okamura, H., Guo, J., Haneji, T."Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells". Molecular Medicine Reports 10, no. 3 (2014): 1371-1376. https://doi.org/10.3892/mmr.2014.2353
Copy and paste a formatted citation
x
Spandidos Publications style
Yu Y, Yang D, Qiu L, Okamura H, Guo J and Haneji T: Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells. Mol Med Rep 10: 1371-1376, 2014.
APA
Yu, Y., Yang, D., Qiu, L., Okamura, H., Guo, J., & Haneji, T. (2014). Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells. Molecular Medicine Reports, 10, 1371-1376. https://doi.org/10.3892/mmr.2014.2353
MLA
Yu, Y., Yang, D., Qiu, L., Okamura, H., Guo, J., Haneji, T."Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells". Molecular Medicine Reports 10.3 (2014): 1371-1376.
Chicago
Yu, Y., Yang, D., Qiu, L., Okamura, H., Guo, J., Haneji, T."Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells". Molecular Medicine Reports 10, no. 3 (2014): 1371-1376. https://doi.org/10.3892/mmr.2014.2353
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