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September-2014 Volume 10 Issue 3

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Article

Transforming growth factor‑β1 induces epithelial‑mesenchymal transition and increased expression of matrix metalloproteinase‑16 via miR‑200b downregulation in bladder cancer cells

  • Authors:
    • Min Feng Chen
    • Feng Zeng
    • Lin Qi
    • Xiong Bing Zu
    • Jun Wang
    • Long Fei Liu
    • Yuan Li
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Xiang Ya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Urology, First Teaching Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Pages: 1549-1554
    |
    Published online on: July 7, 2014
       https://doi.org/10.3892/mmr.2014.2366
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Abstract

Transforming growth factor‑β1 (TGF‑β1) is involved in the migration and metastases of bladder cancer. The present study was designed to investigate whether TGF‑β1 is able to induce epithelial‑mesenchymal transition (EMT) and the upregulation of matrix metalloproteinase‑16 (MMP‑16), and to identify an association between EMT and MMP‑16 in bladder cancer. Following TGF‑β1 treatment, samples of HTB9 and T24 bladder cancer cells were collected at various time points. Western blotting and quantitative polymerase chain reaction (qPCR) confirmed that TGF‑β1 induced EMT in HTB9 and T24 cells at the protein and mRNA levels. The expression levels of the miR‑200 family were determined by qPCR, which indicated that TGF‑β1 treatment significantly reduced the expression of miR‑200b. Bioinformatic analysis indicated that MMP‑16 may be the target of miR‑200b. Reporter luciferase assay confirmed that MMP‑16 is a direct downstream functional target of miR‑200b. A Matrigel migration assay demonstrated that miR‑200b overexpression inhibited the migration of bladder cancer cells. In summary, the current study demonstrated that exogenous TGF‑β1 leads to the induction of EMT and the downregulation of miR‑200b in bladder cancer cells. To the best of our knowledge, this is the first evidence that MMP‑16 is a direct target of miR‑200b.
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Copy and paste a formatted citation
Spandidos Publications style
Chen MF, Zeng F, Qi L, Zu XB, Wang J, Liu LF and Li Y: Transforming growth factor‑β1 induces epithelial‑mesenchymal transition and increased expression of matrix metalloproteinase‑16 via miR‑200b downregulation in bladder cancer cells. Mol Med Rep 10: 1549-1554, 2014.
APA
Chen, M.F., Zeng, F., Qi, L., Zu, X.B., Wang, J., Liu, L.F., & Li, Y. (2014). Transforming growth factor‑β1 induces epithelial‑mesenchymal transition and increased expression of matrix metalloproteinase‑16 via miR‑200b downregulation in bladder cancer cells. Molecular Medicine Reports, 10, 1549-1554. https://doi.org/10.3892/mmr.2014.2366
MLA
Chen, M. F., Zeng, F., Qi, L., Zu, X. B., Wang, J., Liu, L. F., Li, Y."Transforming growth factor‑β1 induces epithelial‑mesenchymal transition and increased expression of matrix metalloproteinase‑16 via miR‑200b downregulation in bladder cancer cells". Molecular Medicine Reports 10.3 (2014): 1549-1554.
Chicago
Chen, M. F., Zeng, F., Qi, L., Zu, X. B., Wang, J., Liu, L. F., Li, Y."Transforming growth factor‑β1 induces epithelial‑mesenchymal transition and increased expression of matrix metalloproteinase‑16 via miR‑200b downregulation in bladder cancer cells". Molecular Medicine Reports 10, no. 3 (2014): 1549-1554. https://doi.org/10.3892/mmr.2014.2366
Copy and paste a formatted citation
x
Spandidos Publications style
Chen MF, Zeng F, Qi L, Zu XB, Wang J, Liu LF and Li Y: Transforming growth factor‑β1 induces epithelial‑mesenchymal transition and increased expression of matrix metalloproteinase‑16 via miR‑200b downregulation in bladder cancer cells. Mol Med Rep 10: 1549-1554, 2014.
APA
Chen, M.F., Zeng, F., Qi, L., Zu, X.B., Wang, J., Liu, L.F., & Li, Y. (2014). Transforming growth factor‑β1 induces epithelial‑mesenchymal transition and increased expression of matrix metalloproteinase‑16 via miR‑200b downregulation in bladder cancer cells. Molecular Medicine Reports, 10, 1549-1554. https://doi.org/10.3892/mmr.2014.2366
MLA
Chen, M. F., Zeng, F., Qi, L., Zu, X. B., Wang, J., Liu, L. F., Li, Y."Transforming growth factor‑β1 induces epithelial‑mesenchymal transition and increased expression of matrix metalloproteinase‑16 via miR‑200b downregulation in bladder cancer cells". Molecular Medicine Reports 10.3 (2014): 1549-1554.
Chicago
Chen, M. F., Zeng, F., Qi, L., Zu, X. B., Wang, J., Liu, L. F., Li, Y."Transforming growth factor‑β1 induces epithelial‑mesenchymal transition and increased expression of matrix metalloproteinase‑16 via miR‑200b downregulation in bladder cancer cells". Molecular Medicine Reports 10, no. 3 (2014): 1549-1554. https://doi.org/10.3892/mmr.2014.2366
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