A SNaPshot assay for the rapid and simple detection of hepatitis B virus genotypes

Lai,Guoqi; Zhang,Wenlu; Tang,Hong; Zhao,Tingting; Wei,Liwen; Tao,Ying; Wang,Zengchan; Huang,Ailong

doi:10.3892/mmr.2014.2372

September-2014 Volume 10 Issue 3

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September-2014 Volume 10 Issue 3

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Article Open Access

A SNaPshot assay for the rapid and simple detection of hepatitis B virus genotypes

Authors:
- Guoqi Lai
- Wenlu Zhang
- Hong Tang
- Tingting Zhao
- Liwen Wei
- Ying Tao
- Zengchan Wang
- Ailong Huang
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Affiliations: Laboratory Animal Center, Chongqing Medical University, Chongqing 400016, P.R. China, Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing 400016, P.R. China

Copyright: © Lai et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 1245-1251
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Published online on: July 14, 2014

https://doi.org/10.3892/mmr.2014.2372
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Abstract

A simple technique for the identification of common genotypes of the hepatitis B virus (HBV) remains to be identified. The present study was conducted to establish such a methodology. Four plasmids of genotypes A‑D and 123 clinical serum specimens of HBV‑infected patients were genotyped. HBV genotypes would be detected successfully when the HBV genotype reached a viral load of 1 x 103 copies/ml or the BC genotype mixed samples reached a 5% level. The lower limit of detection of HBV DNA in serum specimens was determined to be 2.14x102 IU/ml. The assay sensitivity and specificity were 100% and the consistency was demonstrated to reach as high as 90.24 and 100% compared with that of the DNA sequencing and cloning. The frequencies of the genotypes B, C, BC, BD and BCD were found to be 65.0, 23.6, 7.3, 3.3 and 0.8%, respectively. The accuracy of detection of the mixed infections was also higher using the rapid and simple SNaPshot method compared with that achieved with the DNA sequencing methods. The results of the present study indicated that the SNaPshot technique accurately distinguishes the HBV genotypes A‑D and is able to be readily applied as a monitoring tool in HBV prognosis and treatment.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A SNaPshot assay for the rapid and simple detection of hepatitis B virus genotypes

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