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Article

Resveratrol ameliorates low shear stress‑induced oxidative stress by suppressing ERK/eNOS‑Thr495 in endothelial cells

  • Authors:
    • Zhimei Wang
    • Junxia Zhang
    • Bing Li
    • Xiaofei Gao
    • Yanrong Liu
    • Wenxing Mao
    • Shao‑Liang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China
  • Pages: 1964-1972
    |
    Published online on: July 17, 2014
       https://doi.org/10.3892/mmr.2014.2390
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Abstract

Fluid shear stress has been revealed to differentially regulate endothelial nitric oxide synthase (eNOS) distribution in vessels. eNOS, a key enzyme in controlling nitric oxide (NO) release, has a crucial role in mediating oxidative stress, and resveratrol (RSV)‑mediated eNOS also attenuates oxidative damage and suppresses endothelial dysfunction. To observe the protective effect of RSV on low shear stress (LSS)‑induced oxidative damage and the potential mechanisms involved, a parallel‑plate flow chamber, which imposed a low level of stress of 2 dynes/cm2 to cells, was employed. Reactive oxygen species (ROS), NO and apoptotic cells were examined in LSS‑treated endothelial cells (ECs) with or without RSV. Western blot analysis was used to examine LSS‑regulated eNOS‑Ser1177, Thr495 and Ser633, which were tightly associated with NO release. To further determine the underlying signaling pathways involved, extracellular signal‑regulated kinase (ERK), a possible upstream target of eNOS‑Thr495, was investigated, followed by examination of eNOS‑Thr495 in ERK‑inhibited cells. Additionally, eNOS mRNA expression levels were analyzed in cells challenged with LSS. The results revealed that RSV markedly decreased LSS‑induced oxidative damage in ECs. Furthermore, eNOS‑Ser1177 and Thr495 as well as phospho‑ERK were time‑dependently activated by LSS. The ERK inhibitor deactivated eNOS‑Thr495, which was accompanied by increased intracellular superoxide dismutase (SOD) levels. Of note, the activation effect of LSS on ERK/eNOS was markedly eliminated by RSV. In conclusion, RSV exerts antioxidant effects by suppressing LSS‑activated ERK/eNOS and may provide a potential therapeutic target for atherosclerosis.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Zhang J, Li B, Gao X, Liu Y, Mao W and Chen SL: Resveratrol ameliorates low shear stress‑induced oxidative stress by suppressing ERK/eNOS‑Thr495 in endothelial cells. Mol Med Rep 10: 1964-1972, 2014.
APA
Wang, Z., Zhang, J., Li, B., Gao, X., Liu, Y., Mao, W., & Chen, S. (2014). Resveratrol ameliorates low shear stress‑induced oxidative stress by suppressing ERK/eNOS‑Thr495 in endothelial cells. Molecular Medicine Reports, 10, 1964-1972. https://doi.org/10.3892/mmr.2014.2390
MLA
Wang, Z., Zhang, J., Li, B., Gao, X., Liu, Y., Mao, W., Chen, S."Resveratrol ameliorates low shear stress‑induced oxidative stress by suppressing ERK/eNOS‑Thr495 in endothelial cells". Molecular Medicine Reports 10.4 (2014): 1964-1972.
Chicago
Wang, Z., Zhang, J., Li, B., Gao, X., Liu, Y., Mao, W., Chen, S."Resveratrol ameliorates low shear stress‑induced oxidative stress by suppressing ERK/eNOS‑Thr495 in endothelial cells". Molecular Medicine Reports 10, no. 4 (2014): 1964-1972. https://doi.org/10.3892/mmr.2014.2390
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Zhang J, Li B, Gao X, Liu Y, Mao W and Chen SL: Resveratrol ameliorates low shear stress‑induced oxidative stress by suppressing ERK/eNOS‑Thr495 in endothelial cells. Mol Med Rep 10: 1964-1972, 2014.
APA
Wang, Z., Zhang, J., Li, B., Gao, X., Liu, Y., Mao, W., & Chen, S. (2014). Resveratrol ameliorates low shear stress‑induced oxidative stress by suppressing ERK/eNOS‑Thr495 in endothelial cells. Molecular Medicine Reports, 10, 1964-1972. https://doi.org/10.3892/mmr.2014.2390
MLA
Wang, Z., Zhang, J., Li, B., Gao, X., Liu, Y., Mao, W., Chen, S."Resveratrol ameliorates low shear stress‑induced oxidative stress by suppressing ERK/eNOS‑Thr495 in endothelial cells". Molecular Medicine Reports 10.4 (2014): 1964-1972.
Chicago
Wang, Z., Zhang, J., Li, B., Gao, X., Liu, Y., Mao, W., Chen, S."Resveratrol ameliorates low shear stress‑induced oxidative stress by suppressing ERK/eNOS‑Thr495 in endothelial cells". Molecular Medicine Reports 10, no. 4 (2014): 1964-1972. https://doi.org/10.3892/mmr.2014.2390
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