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Effect of ginsenoside Rh2 on the migratory ability of HepG2 liver carcinoma cells: Recruiting histone deacetylase and inhibiting activator protein 1 transcription factors

  • Authors:
    • Qingqiang Shi
    • Jing Li
    • Ziqiang Feng
    • Lvcui Zhao
    • Lian Luo
    • Zhimei  You
    • Danyang Li
    • Jing Xia
    • Guowei Zuo
    • Dilong Chen
  • View Affiliations / Copyright

    Affiliations: Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 40016, P.R. China, Laboratory of Clinical Diagnostics, Chongqing Medical University, Chongqing 40016, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1779-1785
    |
    Published online on: July 18, 2014
       https://doi.org/10.3892/mmr.2014.2392
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Abstract

In previous experiments, ginsenoside Rh2 induced apoptosis and cell cycle arrest, which indicates a potential role for ginsenoside Rh2 in anticancer treatment. The effect of ginsenoside Rh2 on cancer is marked and ginsenoside Rh2 has been shown to inhibit pancreatic tumor migratory ability. In the present study, Transwell chambers were used in order to investigate whether ginsenoside Rh2 inhibits the migratory ability of HepG2 liver carcinoma cells. Furthermore, to analyze activator protein 1 (AP‑1) transcription factor expression following Rh2 treatment, ten plasmids encoding Renilla luciferase coupled to the transcription factors were transiently transfected into the HepG2 cells and luciferase was detected by the Luciferase Reporter Assay system reagent. The results indicated that ginsenoside Rh2 inhibited HepG2 cell migratory ability. The expression levels of AP‑1 transcription factors were increased in HepG2 cells following induction by phorbol 12‑myristate 13‑acetate, but ginsenoside Rh2 suppressed this induced AP‑1 expression. AP‑1 transcription factors recruit histone deacetylase (HDAC)4 and affect its transcription, thus, the expression levels of HDAC4 were also analyzed, and these were found to be increased in the Rh2 treatment group. Matrix metalloproteinase 3 (MMP3), a gene downstream of AP‑1, was then investigated, and the treatment group expressed reduced levels of MMP3 gene and protein. Therefore, the inhibitory effect of ginsenoside Rh2 on the migratory ability of HepG2 may be presumed to occur by the recruitment of HDAC and the resulting inhibition of AP‑1 transcription factors, in order to reduce the expression levels of MMP3 gene and protein.
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1 

Coradini D and Speranza A: Histone deacetylase inhibitors for treatment of hepatocellular carcinoma. Acta Pharmacol Sin. 26:1025–1033. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Quint K, Agaimy A, Di Fazio P, et al: Clinical significance of histone deacetylases 1, 2, 3, and 7: HDAC2 is an independent predictor of survival in HCC. Virchows Arch. 459:129–139. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Page AJ, Cosgrove DC, Philosophe B and Pawlik TM: Hepatocellular carcinoma: diagnosis, management, and prognosis. Surg Oncol Clin N Am. 23:289–311. 2014. View Article : Google Scholar

4 

Zhu Q, Zhou L, Yang Z, et al: O-GlcNAcylation plays a role in tumor recurrence of hepatocellular carcinoma following liver transplantation. Med Oncol. 29:985–993. 2012. View Article : Google Scholar

5 

Ji Z, Wang T, Shao Z, et al: A population-based study examining hepatitis B virus infection and immunization rates in Northwest China. PLoS One. 9:e974742014. View Article : Google Scholar

6 

Scaggiante B, Kazemi M, Pozzato G, et al: Novel hepatocellular carcinoma molecules with prognostic and therapeutic potentials. World J Gastroenterol. 20:1268–1288. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Chung KS, Cho SH, Shin JS, et al: Ginsenoside Rh2 induces cell cycle arrest and differentiation in human leukemia cells by upregulating TGF-β expression. Carcinogenesis. 34:331–340. 2013.PubMed/NCBI

8 

Christensen LP: Ginsenosides chemistry, biosynthesis, analysis, and potential health effects. Adv Food Nutr Res. 55:1–99. 2009.PubMed/NCBI

9 

Tang XP, Tang GD, Fang CY, Liang ZH and Zhang LY: Effects of ginsenoside Rh2 on growth and migration of pancreatic cancer cells. World J Gastroenterol. 19:1582–1592. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Wang Z, Zheng Q, Liu K, Li G and Zheng R: Ginsenoside Rh(2) enhances antitumour activity and decreases genotoxic effect of cyclophosphamide. Basic Clin Pharmacol Toxicol. 98:411–415. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Cheng CC, Yang SM, Huang CY, Chen JC, Chang WM and Hsu SL: Molecular mechanisms of ginsenoside Rh2-mediated G1 growth arrest and apoptosis in human lung adenocarcinoma A549 cells. Cancer Chemother Pharmacol. 55:531–540. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Eferl R and Wagner EF: AP-1: a double-edged sword in tumorigenesis. Nat Rev Cancer. 3:859–868. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Mittelstadt ML and Patel RC: AP-1 mediated transcriptional repression of matrix metalloproteinase-9 by recruitment of histone deacetylase 1 in response to interferon β. PLoS One. 7:e421522012.PubMed/NCBI

14 

Wang L, Cheng X, Li H, et al: Quercetin reduces oxidative stress and inhibits activation of c-Jun N-terminal kinase/activator protein-1 signaling in an experimental mouse model of abdominal aortic aneurysm. Mol Med Rep. 9:435–442. 2014.

15 

Eckert RL, Adhikary G, Young CA, et al: AP1 transcription factors in epidermal differentiation and skin cancer. J Skin Cancer. 2013:5370282013. View Article : Google Scholar : PubMed/NCBI

16 

Barbetti V, Gozzini A, Cheloni G, et al: Time- and residue-specific differences in histone acetylation induced by VPA and SAHA in AML1/ETO-positive leukemia cells. Epigenetics. 8:210–219. 2013. View Article : Google Scholar

17 

Zhang M, Dai C, Zhu H, et al: Cyclophilin A promotes human hepatocellular carcinoma cell metastasis via regulation of MMP3 and MMP9. Mol Cell Biochem. 357:387–395. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Zhang C, Li C, Zhu M, et al: Meta-analysis of MMP2, MMP3, and MMP9 promoter polymorphisms and head and neck cancer risk. PLoS One. 8:e620232013. View Article : Google Scholar : PubMed/NCBI

19 

Mendes O, Kim HT and Stoica G: Expression of MMP2, MMP9 and MMP3 in breast cancer brain metastasis in a rat model. Clin Exp Metastasis. 22:237–246. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Fanjul-Fernández M, Folgueras AR, Cabrera S and López-Otín C: Matrix metalloproteinases: evolution, gene regulation and functional analysis in mouse models. Biochim Biophys Acta. 1803.3–19. 2010.PubMed/NCBI

21 

Théret N, Musso O, Turlin B, et al: Increased extracellular matrix remodeling is associated with tumor progression in human hepatocellular carcinomas. Hepatology. 34:82–88. 2001.PubMed/NCBI

22 

Slattery ML, John E, Torres-Mejia G, et al: Matrix metalloproteinase genes are associated with breast cancer risk and survival: the Breast Cancer Health Disparities Study. PLoS One. 8:e631652013. View Article : Google Scholar

23 

Ishii Y, Nakasato Y, Kobayashi S, Yamazaki Y and Aoki T: A study on angiogenesis-related matrix metalloproteinase networks in primary hepatocellular carcinoma. J Exp Clin Cancer Res. 22:461–470. 2003.

24 

Guo XX, Guo Q, Li Y, Lee SK, Wei XN and Jin YH: Ginsenoside rh2 induces human hepatoma cell apoptosisvia bax/bak triggered cytochrome C release and caspase-9/caspase-8 activation. Int J Mol Sci. 13:15523–15535. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Park HM, Kim SJ, Kim JS and Kang HS: Reactive oxygen species mediated ginsenoside Rg3- and Rh2-induced apoptosis in hepatoma cells through mitochondrial signaling pathways. Food Chem Toxicol. 50:2736–2741. 2012. View Article : Google Scholar

26 

Naylor LH: Reporter gene technology: the future looks bright. Biochem Pharmacol. 58:749–757. 1999. View Article : Google Scholar : PubMed/NCBI

27 

Brasier AR, Tate JE and Habener JF: Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines. Biotechniques. 7:1116–1122. 1989.PubMed/NCBI

28 

Eckert RL, Adhikary G, Young CA, et al: AP1 transcription factors in epidermal differentiation and skin cancer. J Skin Cancer. 2013:5370282013. View Article : Google Scholar : PubMed/NCBI

29 

Miotto B, Sagnier T, Berenger H, Bohmann D, Pradel J and Graba Y: Chameau HAT and DRpd3 HDAC function as antagonistic cofactors of JNK/AP-1-dependent transcription during Drosophila metamorphosis. Genes Dev. 20:101–112. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Correia AL, Mori H, Chen EI, Schmitt FC and Bissell MJ: The hemopexin domain of MMP3 is responsible for mammary epithelial invasion and morphogenesis through extracellular interaction with HSP90β. Genes Dev. 27:805–817. 2013.PubMed/NCBI

31 

Choi MC, Cohen TJ, Barrientos T, et al: A direct HDAC4-MAP kinase crosstalk activates muscle atrophy program. Mol Cell. 47:122–132. 2012.PubMed/NCBI

32 

Mendes O, Kim HT and Stoica G: Expression of MMP2, MMP9 and MMP3 in breast cancer brain metastasis in a rat model. Clin Exp Metastasis. 22:237–246. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Fanjul-Fernández M, Folgueras AR, Cabrera S and López-Otín C: Matrix metalloproteinases: evolution, gene regulation and functional analysis in mouse models. Biochim Biophys Acta. 1803.3–19. 2010.PubMed/NCBI

34 

Butticè G, Duterque-Coquillaud M, Basuyaux JP, Carrère S, Kurkinen M and Stéhelin D: Erg, an Ets-family member, differentially regulates human collagenase1 (MMP1) and stromelysin1 (MMP3) gene expression by physically interacting with the Fos/Jun complex. Oncogene. 13:2297–2306. 1996.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shi Q, Li J, Feng Z, Zhao L, Luo L, You Z, Li D, Xia J, Zuo G, Chen D, Chen D, et al: Effect of ginsenoside Rh2 on the migratory ability of HepG2 liver carcinoma cells: Recruiting histone deacetylase and inhibiting activator protein 1 transcription factors. Mol Med Rep 10: 1779-1785, 2014.
APA
Shi, Q., Li, J., Feng, Z., Zhao, L., Luo, L., You, Z. ... Chen, D. (2014). Effect of ginsenoside Rh2 on the migratory ability of HepG2 liver carcinoma cells: Recruiting histone deacetylase and inhibiting activator protein 1 transcription factors. Molecular Medicine Reports, 10, 1779-1785. https://doi.org/10.3892/mmr.2014.2392
MLA
Shi, Q., Li, J., Feng, Z., Zhao, L., Luo, L., You, Z., Li, D., Xia, J., Zuo, G., Chen, D."Effect of ginsenoside Rh2 on the migratory ability of HepG2 liver carcinoma cells: Recruiting histone deacetylase and inhibiting activator protein 1 transcription factors". Molecular Medicine Reports 10.4 (2014): 1779-1785.
Chicago
Shi, Q., Li, J., Feng, Z., Zhao, L., Luo, L., You, Z., Li, D., Xia, J., Zuo, G., Chen, D."Effect of ginsenoside Rh2 on the migratory ability of HepG2 liver carcinoma cells: Recruiting histone deacetylase and inhibiting activator protein 1 transcription factors". Molecular Medicine Reports 10, no. 4 (2014): 1779-1785. https://doi.org/10.3892/mmr.2014.2392
Copy and paste a formatted citation
x
Spandidos Publications style
Shi Q, Li J, Feng Z, Zhao L, Luo L, You Z, Li D, Xia J, Zuo G, Chen D, Chen D, et al: Effect of ginsenoside Rh2 on the migratory ability of HepG2 liver carcinoma cells: Recruiting histone deacetylase and inhibiting activator protein 1 transcription factors. Mol Med Rep 10: 1779-1785, 2014.
APA
Shi, Q., Li, J., Feng, Z., Zhao, L., Luo, L., You, Z. ... Chen, D. (2014). Effect of ginsenoside Rh2 on the migratory ability of HepG2 liver carcinoma cells: Recruiting histone deacetylase and inhibiting activator protein 1 transcription factors. Molecular Medicine Reports, 10, 1779-1785. https://doi.org/10.3892/mmr.2014.2392
MLA
Shi, Q., Li, J., Feng, Z., Zhao, L., Luo, L., You, Z., Li, D., Xia, J., Zuo, G., Chen, D."Effect of ginsenoside Rh2 on the migratory ability of HepG2 liver carcinoma cells: Recruiting histone deacetylase and inhibiting activator protein 1 transcription factors". Molecular Medicine Reports 10.4 (2014): 1779-1785.
Chicago
Shi, Q., Li, J., Feng, Z., Zhao, L., Luo, L., You, Z., Li, D., Xia, J., Zuo, G., Chen, D."Effect of ginsenoside Rh2 on the migratory ability of HepG2 liver carcinoma cells: Recruiting histone deacetylase and inhibiting activator protein 1 transcription factors". Molecular Medicine Reports 10, no. 4 (2014): 1779-1785. https://doi.org/10.3892/mmr.2014.2392
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