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Combined effect of nerve growth factor and brain‑derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms

  • Authors:
    • Feifei Liu
    • Aiguo Xuan
    • Yan Chen
    • Jundu Zhang
    • Liping Xu
    • Qijiang Yan
    • Dahong Long
  • View Affiliations / Copyright

    Affiliations: Department of Human Anatomy, Guangzhou Medical University, Guangzhou, Guangdong 510182, P.R. China, Kingmed Diagnostics College, Guangzhou Medical University, Guangzhou, Guangdong 510182, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1739-1745
    |
    Published online on: July 18, 2014
       https://doi.org/10.3892/mmr.2014.2393
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Abstract

Neural stem cells (NSCs) are important pluripotent stem cells, which have potential applications in cell replacement therapy. Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) have been demonstrated to exert a marked impact on the proliferation and differentiation of NSCs. The effects of NGF, BDNF, and BDNF combined with NGF on NSC neuronal differentiation and the possible mechanisms for these effects were investigated in this study. An adherent monolayer culture was employed to obtain highly homogeneous NSCs. The cells were divided into four groups: Control, NGF, BDNF and combination (BDNF + NGF) groups. Neuron differentiation was examined using immunocytochemistry and phospho-extracellular signal‑regulated kinase (p‑ERK) levels were analyzed using western blotting. Reverse transcription polymerase chain reaction was used to measure the mRNA expression levels of the HES1, HES5, MASH1, NGN1 and NeuroD transcription factors at different time intervals following neurotrophin‑induced differentiation. NGF and BDNF were observed to induce NSC neuronal differentiation, and β-tubulin III-positive cells and p-ERK expression levels were highest in the NGF + BDNF combination group at all time points. The proportion of β-tubulin Ⅲ‑positive neurons in each group was associated with the expression levels of MASH1, NGN1 and NeuroD in the group. In conclusion, BDNF combined with NGF significantly improved NSC neuronal differentiation, which may provide support for the practical application of NSCs in neurodegenerative diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Liu F, Xuan A, Chen Y, Zhang J, Xu L, Yan Q and Long D: Combined effect of nerve growth factor and brain‑derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms. Mol Med Rep 10: 1739-1745, 2014.
APA
Liu, F., Xuan, A., Chen, Y., Zhang, J., Xu, L., Yan, Q., & Long, D. (2014). Combined effect of nerve growth factor and brain‑derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms. Molecular Medicine Reports, 10, 1739-1745. https://doi.org/10.3892/mmr.2014.2393
MLA
Liu, F., Xuan, A., Chen, Y., Zhang, J., Xu, L., Yan, Q., Long, D."Combined effect of nerve growth factor and brain‑derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms". Molecular Medicine Reports 10.4 (2014): 1739-1745.
Chicago
Liu, F., Xuan, A., Chen, Y., Zhang, J., Xu, L., Yan, Q., Long, D."Combined effect of nerve growth factor and brain‑derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms". Molecular Medicine Reports 10, no. 4 (2014): 1739-1745. https://doi.org/10.3892/mmr.2014.2393
Copy and paste a formatted citation
x
Spandidos Publications style
Liu F, Xuan A, Chen Y, Zhang J, Xu L, Yan Q and Long D: Combined effect of nerve growth factor and brain‑derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms. Mol Med Rep 10: 1739-1745, 2014.
APA
Liu, F., Xuan, A., Chen, Y., Zhang, J., Xu, L., Yan, Q., & Long, D. (2014). Combined effect of nerve growth factor and brain‑derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms. Molecular Medicine Reports, 10, 1739-1745. https://doi.org/10.3892/mmr.2014.2393
MLA
Liu, F., Xuan, A., Chen, Y., Zhang, J., Xu, L., Yan, Q., Long, D."Combined effect of nerve growth factor and brain‑derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms". Molecular Medicine Reports 10.4 (2014): 1739-1745.
Chicago
Liu, F., Xuan, A., Chen, Y., Zhang, J., Xu, L., Yan, Q., Long, D."Combined effect of nerve growth factor and brain‑derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms". Molecular Medicine Reports 10, no. 4 (2014): 1739-1745. https://doi.org/10.3892/mmr.2014.2393
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