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Article Open Access

Resveratrol inhibits proliferation in human colorectal carcinoma cells by inducing G1/S‑phase cell cycle arrest and apoptosis through caspase/cyclin‑CDK pathways

  • Authors:
    • Bin Liu
    • Zhongyou Zhou
    • Wei Zhou
    • Jie Liu
    • Qingyu Zhang
    • Juan  Xia
    • Juntao Liu
    • Nianping Chen
    • Mingyi Li
    • Runzhi Zhu
  • View Affiliations / Copyright

    Affiliations: Laboratory of Hepatobiliary Surgery, Guangdong Medical College, Zhanjiang Key Laboratory of Hepatobiliary Diseases, Zhanjiang, Guangdong 524001, P.R. China, Department of Radiology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1697-1702
    |
    Published online on: July 21, 2014
       https://doi.org/10.3892/mmr.2014.2406
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Abstract

The present study compared the effect of resveratrol on HCT116 and Caco‑2 human colon cancer cells. Annexin V/propidium iodide staining, MTT assay and western blot analysis revealed that resveratrol induced cycle arrest in the two cell lines, which was evidenced by cell cycle analysis and changes in the expression of the cell cycle proteins cyclin‑dependent kinase (CDK) 2, CDK4, cyclin D1, proliferating cell nuclear antigen and P21. Furthermore, resveratrol was found to have a strong apoptosis‑inducing effect, which was evidenced through the high percentage of annexin V positive cells and high protein expression of cleaved‑caspase‑7, cleaved‑caspase‑9 and cleaved‑poly(ADP‑ribose) polymerase in the resveratrol‑treated cancer cells. In conclusion, these results demonstrated that resveratrol had greater growth inhibitory and cell cycle arrest effects on Caco‑2 cells than HCT116 cells, through caspase‑dependent and cyclin‑CDK pathways.
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1 

Fulda S: Resveratrol and derivatives for the prevention and treatment of cancer. Drug Discov Today. 15:757–765. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Calabrese EJ, Mattson MP and Calabrese V: Dose response biology: the case of resveratrol. Hum Exp Toxicol. 29:1034–1037. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Park SJ, Ahmad F, Philp A, et al: Resveratrol ameliorates aging-related metabolic phenotypes by inhibiting cAMP phosphodiesterases. Cell. 148:421–433. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Szekeres T, Fritzer-Szekeres M, Saiko P and Jäger W: Resveratrol and resveratrol analogues - structure-activity relationship. Pharm Res. 27:1042–1048. 2010. View Article : Google Scholar

5 

Szekeres T, Saiko P, Fritzer-Szekeres M, Djavan B and Jäger W: Chemopreventive effects of resveratrol and resveratrol derivatives. Ann NY Acad Sci. 1215:89–95. 2011. View Article : Google Scholar

6 

Athar M, Back JH, Kopelovich L, Bickers DR and Kim AL: Multiple molecular targets of resveratrol: Anti-carcinogenic mechanisms. Arch Biochem Biophys. 486:95–102. 2009. View Article : Google Scholar

7 

Tennen RI, Michishita-Kioi E and Chua KF: Finding a target for resveratrol. Cell. 148:387–389. 2012. View Article : Google Scholar

8 

Jemal A, Siegel R, Xu J and Ward E: Cancer statistics, 2010. CA Cancer J Clin. 60:277–300. 2010. View Article : Google Scholar

9 

Siegel RL, Jemal A and Ward EM: Increase in incidence of colorectal cancer among young men and women in the United States. Cancer Epidemiol Biomarkers Prev. 18:1695–1698. 2009. View Article : Google Scholar

10 

De la Rosa L, Alvarez-Parrilla E and González-Aguilar GA: Fruit and vegetable phytochemicals: Chemistry, nutritional value and stability Phenolic Compounds: Chemistry and Occurrence in Fruits and Vegetables. Wiley-Blackwell, EUA; pp. 53–88. 2010

11 

Agarwal S, Arya V and Zhang L: Review of P-gp Inhibition data in recently approved new drug applications: utility of the proposed [I(1) ]/IC(50) and [I(2) ]/IC(50) criteria in the P-gp decision tree. J Clin Pharmacol. 53:228–233. 2013.

12 

Nutakul W, Sobers HS, Qiu P, et al: Inhibitory effects of resveratrol and pterostilbene on human colon cancer cells: a side-by-side comparison. J Agric Food Chem. 59:10964–10970. 2011. View Article : Google Scholar

13 

Piotrowska H, Kucinska M and Murias M: Biological activity of piceatannol: leaving the shadow of resveratrol. Mutat Res. 750:60–82. 2012. View Article : Google Scholar : PubMed/NCBI

14 

de Athayde Moncorvo Collado A, Corbalán N, Homolya L, Morero R and Minahk C: Resveratrol modulates ATPase activity of liposome-reconstituted ABCG1. FEBS Lett. 587:2359–2363. 2013.PubMed/NCBI

15 

Um JH, Park SJ, Kang H, et al: AMP-activated protein kinase-deficient mice are resistant to the metabolic effects of resveratrol. Diabetes. 59:554–563. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Yasuda Y, Saito M, Yamamura T, Yaguchi T and Nishizaki T: Extracellular adenosine induces apoptosis in Caco-2 human colonic cancer cells by activating caspase-9/−3 via A(2a) adenosine receptors. J Gastroenterol. 44:56–65. 2009.

17 

Allan LA and Clarke PR: Apoptosis and autophagy: Regulation of caspase-9 by phosphorylation. FEBS J. 276:6063–6073. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Boucher D, Blais V and Denault JB: Caspase-7 uses an exosite to promote poly(ADP ribose) polymerase 1 proteolysis. Proc Natl Acad Sci USA. 109:5669–5674. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Rodríguez-Berriguete G, Galvis L, Fraile B, et al: Immunoreactivity to caspase-3, caspase-7, caspase-8, and caspase-9 forms is frequently lost in human prostate tumors. Hum Pathol. 43:229–237. 2012.PubMed/NCBI

20 

Peralta-Leal A, Rodríguez-Vargas JM, Aguilar-Quesada R, et al: PARP inhibitors: new partners in the therapy of cancer and inflammatory diseases. Free Radic Biol Med. 47:13–26. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Galluzzi L, Vitale I, Abrams JM, et al: Molecular definitions of cell death subroutines: recommendations of the Nomenclature Committee on Cell Death 2012. Cell Death Differ. 19:107–120. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Langelier M-F and Pascal JM: PARP-1 mechanism for coupling DNA damage detection to poly(ADP-ribose) synthesis. Curr Opin Struct Biol. 23:134–143. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Yang L, Zhang HW, Hu R, et al: Wogonin induces G1 phase arrest through inhibiting Cdk4 and cyclin D1 concomitant with an elevation in p21Cip1 in human cervical carcinoma HeLa cells. Biochem Cell Biol. 87:933–942. 2009. View Article : Google Scholar

24 

Kim MY: Nitric oxide triggers apoptosis in A375 human melanoma cells treated with capsaicin and resveratrol. Mol Med Rep. 5:585–591. 2012.PubMed/NCBI

25 

Majumdar AP, Banerjee S, Nautiyal J, et al: Curcumin synergizes with resveratrol to inhibit colon cancer. Nutr Cancer. 61:544–553. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Soria G and Gottifredi V: PCNA-coupled p21 degradation after DNA damage: The exception that confirms the rule? DNA Repair (Amst). 9:358–364. 2010. View Article : Google Scholar

27 

Beckerman R, Donner AJ, Mattia M, et al: A role for Chk1 in blocking transcriptional elongation of p21 RNA during the S-phase checkpoint. Genes Dev. 23:1364–1377. 2009. View Article : Google Scholar

28 

Adon AM, Zeng X, Harrison MK, et al: Cdk2 and Cdk4 regulate the centrosome cycle and are critical mediators of centrosome amplification in p53-null cells. Mol Cell Biol. 30:694–710. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu B, Zhou Z, Zhou W, Liu J, Zhang Q, Xia J, Liu J, Chen N, Li M, Zhu R, Zhu R, et al: Resveratrol inhibits proliferation in human colorectal carcinoma cells by inducing G1/S‑phase cell cycle arrest and apoptosis through caspase/cyclin‑CDK pathways. Mol Med Rep 10: 1697-1702, 2014.
APA
Liu, B., Zhou, Z., Zhou, W., Liu, J., Zhang, Q., Xia, J. ... Zhu, R. (2014). Resveratrol inhibits proliferation in human colorectal carcinoma cells by inducing G1/S‑phase cell cycle arrest and apoptosis through caspase/cyclin‑CDK pathways. Molecular Medicine Reports, 10, 1697-1702. https://doi.org/10.3892/mmr.2014.2406
MLA
Liu, B., Zhou, Z., Zhou, W., Liu, J., Zhang, Q., Xia, J., Liu, J., Chen, N., Li, M., Zhu, R."Resveratrol inhibits proliferation in human colorectal carcinoma cells by inducing G1/S‑phase cell cycle arrest and apoptosis through caspase/cyclin‑CDK pathways". Molecular Medicine Reports 10.4 (2014): 1697-1702.
Chicago
Liu, B., Zhou, Z., Zhou, W., Liu, J., Zhang, Q., Xia, J., Liu, J., Chen, N., Li, M., Zhu, R."Resveratrol inhibits proliferation in human colorectal carcinoma cells by inducing G1/S‑phase cell cycle arrest and apoptosis through caspase/cyclin‑CDK pathways". Molecular Medicine Reports 10, no. 4 (2014): 1697-1702. https://doi.org/10.3892/mmr.2014.2406
Copy and paste a formatted citation
x
Spandidos Publications style
Liu B, Zhou Z, Zhou W, Liu J, Zhang Q, Xia J, Liu J, Chen N, Li M, Zhu R, Zhu R, et al: Resveratrol inhibits proliferation in human colorectal carcinoma cells by inducing G1/S‑phase cell cycle arrest and apoptosis through caspase/cyclin‑CDK pathways. Mol Med Rep 10: 1697-1702, 2014.
APA
Liu, B., Zhou, Z., Zhou, W., Liu, J., Zhang, Q., Xia, J. ... Zhu, R. (2014). Resveratrol inhibits proliferation in human colorectal carcinoma cells by inducing G1/S‑phase cell cycle arrest and apoptosis through caspase/cyclin‑CDK pathways. Molecular Medicine Reports, 10, 1697-1702. https://doi.org/10.3892/mmr.2014.2406
MLA
Liu, B., Zhou, Z., Zhou, W., Liu, J., Zhang, Q., Xia, J., Liu, J., Chen, N., Li, M., Zhu, R."Resveratrol inhibits proliferation in human colorectal carcinoma cells by inducing G1/S‑phase cell cycle arrest and apoptosis through caspase/cyclin‑CDK pathways". Molecular Medicine Reports 10.4 (2014): 1697-1702.
Chicago
Liu, B., Zhou, Z., Zhou, W., Liu, J., Zhang, Q., Xia, J., Liu, J., Chen, N., Li, M., Zhu, R."Resveratrol inhibits proliferation in human colorectal carcinoma cells by inducing G1/S‑phase cell cycle arrest and apoptosis through caspase/cyclin‑CDK pathways". Molecular Medicine Reports 10, no. 4 (2014): 1697-1702. https://doi.org/10.3892/mmr.2014.2406
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