Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
October 2014 Volume 10 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October 2014 Volume 10 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Microarray analysis of long non-coding RNA expression in human acute rejection biopsy samples following renal transplantation

  • Authors:
    • Wenbiao Chen
    • Wujian Peng
    • Jianrong Huang
    • Xiangqi Yu
    • Kuibi Tan
    • Yuyu Chen
    • Xiaocong Lin
    • Deheng Chen
    • Yong Dai
  • View Affiliations / Copyright

    Affiliations: Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China, Third People's Hospital of Shenzhen, Guangdong Medical College, Shenzhen, Guangdong 518112, P.R. China, Ningbo Second Hospital, Ningbo, Zhejiang 315100, P.R. China, Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang, Guangdong 524000, P.R. China
  • Pages: 2210-2216
    |
    Published online on: July 24, 2014
       https://doi.org/10.3892/mmr.2014.2420
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Rejection is still a major obstacle in long-term allograft survival of renal transplant recipients. Long non‑coding RNAs (lncRNAs) are an important class of pervasive RNAs involved in a variety of biological functions, and which are often found to be differentially expressed between healthy and pathological conditions. The aim of this study was to compare the expression profiles of lncRNAs between samples from acute rejection following kidney transplantation and control samples. Three patients were enrolled, diagnosed by renal biopsy with acute rejection upon kidney transplantation. We used lncRNA microarrays to study the lncRNA expression profiles in the kidney biopsies of these patients and in kidneys from healthy donors. Reverse transcription‑quantitative polymerase chain reaction (RT-qPCR) was used to validate the microarray results. In addition, potential functions of the identified lncRNAs were further explored by searching the UCSC, RNAdb, RefSeq and NRED databases. Five candidate lncRNAs displaying differential expression in acute rejection samples were validated by RT-qPCR. The results were in agreement with the microarray data. Among the identified lncRNAs, certain have been previously identified in relevant conditions, thereby supporting previous evidence, but certain may constitute novel biomarker candidates. This is the first report to date using lncRNA microarrays to identify unique expression signatures of acute rejection in transplant biopsies. Our data indicate that lncRNAs are potentially involved in the pathogenesis of acute rejection. Our results may have important implications in the identification of diagnostic biomarkers, as well as in the understanding and treatment of acute rejection following renal transplantation.
View Figures

Figure 1

View References

1 

Ekberg H, Bernasconi C, Tedesco-Silva H, et al: Calcineurin inhibitor minimization in the Symphony study: observational results 3 years after transplantation. Am J Transplant. 9:1876–1885. 2009.PubMed/NCBI

2 

Shoskes DA and Cecka JM: Deleterious effects of delayed graft function in cadaveric renal transplant recipients independent of acute rejection. Transplantation. 66:1697–1701. 1998. View Article : Google Scholar : PubMed/NCBI

3 

Hariharan S, Johnson CP, Bresnahan BA, Taranto SE, McIntosh MJ and Stablein D: Improved graft survival after renal transplantation in the United States, 1988 to 1996. N Engl J Med. 342:605–612. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Meier-Kriesche HU, Ojo AO, Hanson JA, et al: Increased impact of acute rejection on chronic allograft failure in recent era. Transplantation. 70:1098–1100. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Paul LC: Chronic allograft nephropathy: an update. Kidney Int. 56:783–793. 1999. View Article : Google Scholar : PubMed/NCBI

6 

Humar A, Hassoun A, Kandaswamy R, Payne WD, Sutherland DE and Matas AJ: Immunologic factors: the major risk for decreased long-term renal allograft survival. Transplantation. 68:1842–1846. 1999. View Article : Google Scholar : PubMed/NCBI

7 

Cosio FG, Pelletier RP, Falkenhain ME, et al: Impact of acute rejection and early allograft function on renal allograft survival. Transplantation. 63:1611–1615. 1997. View Article : Google Scholar : PubMed/NCBI

8 

Woodroffe R, Yao GL, Meads C, et al: Clinical and cost-effectiveness of newer immunosuppressive regimens in renal transplantation: a systematic review and modelling study. Health Technol Assess. 9:1–179. iii–iv. 2005.PubMed/NCBI

9 

Racusen LC, Solez K, Colvin RB, et al: The Banff 97 working classification of renal allograft pathology. Kidney Int. 55:713–723. 1999. View Article : Google Scholar : PubMed/NCBI

10 

Howie AJ: Problems with BANFF. Transplantation. 73:1383–1384. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Ramanathan V, Goral S and Helderman JH: Renal transplantation. Semin Nephrol. 21:213–219. 2001. View Article : Google Scholar

12 

Girlanda R, Mannon RB and Kirk AD: Diagnostic tools for monitoring kidney transplant recipients. Semin Nephrol. 27:462–478. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Perkins D, Verma M and Park KJ: Advances of genomic science and systems biology in renal transplantation: a review. Semin Immunopathol. 33:211–218. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Sui W, Dai Y, Huang Y, Lan H, Yan Q and Huang H: Microarray analysis of microRNA expression in acute rejection after renal transplantation. Transpl Immunol. 19:81–85. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Zand MS: Immunosuppression and immune monitoring after renal transplantation. Semin Dial. 18:511–519. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Mercer TR, Dinger ME and Mattick JS: Long non-coding RNAs: insights into functions. Nat Rev Genet. 10:155–159. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Feng J, Bi C, Clark BS, Mady R, Shah P and Kohtz JD: The Evf-2 noncoding RNA is transcribed from the Dlx-5/6 ultraconserved region and functions as a Dlx-2 transcriptional coactivator. Genes Dev. 20:1470–1484. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Yan MD, Hong CC, Lai GM, Cheng AL, Lin YW and Chuang SE: Identification and characterization of a novel gene Saf transcribed from the opposite strand of Fas. Hum Mol Genet. 14:1465–1474. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Wang H, Iacoangeli A, Lin D, et al: Dendritic BC1 RNA in translational control mechanisms. J Cell Biol. 171:811–821. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Sleutels F, Zwart R and Barlow DP: The non-coding Air RNA is required for silencing autosomal imprinted genes. Nature. 415:810–813. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Mercer TR, Dinger ME, Sunkin SM, Mehler MF and Mattick JS: Specific expression of long noncoding RNAs in the mouse brain. Proc Natl Acad Sci USA. 105:716–721. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Dinger ME, Amaral PP, Mercer TR, et al: Long noncoding RNAs in mouse embryonic stem cell pluripotency and differentiation. Genome Res. 18:1433–1445. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Pang KC, Dinger ME, Mercer TR, et al: Genome-wide identification of long noncoding RNAs in CD8+ T cells. J Immunol. 182:7738–7748. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Groos S, Reale E and Luciano L: Re-evaluation of epoxy resin sections for light and electron microscopic immunostaining. J Histochem Cytochem. 49:397–406. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Schmittgen TD and Livak KJ: Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 3:1101–1108. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Sharma VK, Bologa RM, Li B, et al: Molecular executors of cell death - differential intrarenal expression of Fas ligand, Fas, granzyme B, and perforin during acute and/or chronic rejection of human renal allografts. Transplantation. 62:1860–1866. 1996. View Article : Google Scholar

27 

McLean AG, Hughes D, Welsh KI, et al: Patterns of graft infiltration and cytokine gene expression during the first 10 days of kidney transplantation. Transplantation. 63:374–380. 1997. View Article : Google Scholar : PubMed/NCBI

28 

Wilson CB, Makar KW, Shnyreva M and Fitzpatrick DR: DNA methylation and the expanding epigenetics of T cell lineage commitment. Semin Immunol. 17:105–119. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Mattick JS, Amaral PP, Dinger ME, Mercer TR and Mehler MF: RNA regulation of epigenetic processes. Bioessays. 31:51–59. 2009. View Article : Google Scholar

30 

Louro R, Smirnova AS and Verjovski-Almeida S: Long intronic noncoding RNA transcription: expression noise or expression choice? Genomics. 93:291–298. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Harrison EM, Sharpe E, Bellamy CO, et al: Heat shock protein 90-binding agents protect renal cells from oxidative stress and reduce kidney ischemia-reperfusion injury. Am J Physiol Renal Physiol. 295:F397–F405. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Goral J, Shenoy S, Mohanakumar T and Clancy J Jr: Antibodies to 70 kD and 90 kD heat shock proteins are associated with graft-versus-host disease in peripheral blood stem cell transplant recipients. Clin Exp Immunol. 127:553–559. 2002. View Article : Google Scholar

33 

Markiewski MM, DeAngelis RA and Lambris JD: Complexity of complement activation in sepsis. J Cell Mol Med. 12:2245–2254. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Ioannou A, Dalle Lucca J and Tsokos GC: Immunopathogenesis of ischemia/reperfusion-associated tissue damage. Clin Immunol. 141:3–14. 2011. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen W, Peng W, Huang J, Yu X, Tan K, Chen Y, Lin X, Chen D and Dai Y: Microarray analysis of long non-coding RNA expression in human acute rejection biopsy samples following renal transplantation. Mol Med Rep 10: 2210-2216, 2014.
APA
Chen, W., Peng, W., Huang, J., Yu, X., Tan, K., Chen, Y. ... Dai, Y. (2014). Microarray analysis of long non-coding RNA expression in human acute rejection biopsy samples following renal transplantation. Molecular Medicine Reports, 10, 2210-2216. https://doi.org/10.3892/mmr.2014.2420
MLA
Chen, W., Peng, W., Huang, J., Yu, X., Tan, K., Chen, Y., Lin, X., Chen, D., Dai, Y."Microarray analysis of long non-coding RNA expression in human acute rejection biopsy samples following renal transplantation". Molecular Medicine Reports 10.4 (2014): 2210-2216.
Chicago
Chen, W., Peng, W., Huang, J., Yu, X., Tan, K., Chen, Y., Lin, X., Chen, D., Dai, Y."Microarray analysis of long non-coding RNA expression in human acute rejection biopsy samples following renal transplantation". Molecular Medicine Reports 10, no. 4 (2014): 2210-2216. https://doi.org/10.3892/mmr.2014.2420
Copy and paste a formatted citation
x
Spandidos Publications style
Chen W, Peng W, Huang J, Yu X, Tan K, Chen Y, Lin X, Chen D and Dai Y: Microarray analysis of long non-coding RNA expression in human acute rejection biopsy samples following renal transplantation. Mol Med Rep 10: 2210-2216, 2014.
APA
Chen, W., Peng, W., Huang, J., Yu, X., Tan, K., Chen, Y. ... Dai, Y. (2014). Microarray analysis of long non-coding RNA expression in human acute rejection biopsy samples following renal transplantation. Molecular Medicine Reports, 10, 2210-2216. https://doi.org/10.3892/mmr.2014.2420
MLA
Chen, W., Peng, W., Huang, J., Yu, X., Tan, K., Chen, Y., Lin, X., Chen, D., Dai, Y."Microarray analysis of long non-coding RNA expression in human acute rejection biopsy samples following renal transplantation". Molecular Medicine Reports 10.4 (2014): 2210-2216.
Chicago
Chen, W., Peng, W., Huang, J., Yu, X., Tan, K., Chen, Y., Lin, X., Chen, D., Dai, Y."Microarray analysis of long non-coding RNA expression in human acute rejection biopsy samples following renal transplantation". Molecular Medicine Reports 10, no. 4 (2014): 2210-2216. https://doi.org/10.3892/mmr.2014.2420
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team