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Molecular Medicine Reports
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October 2014 Volume 10 Issue 4

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Article Open Access

siRNA‑induced ABCE1 silencing inhibits proliferation and invasion of breast cancer cells

  • Authors:
    • Bo Huang
    • Hongli Zhou
    • Xianping Lang
    • Zhiliang Liu
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Urology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1685-1690
    |
    Published online on: July 28, 2014
       https://doi.org/10.3892/mmr.2014.2424
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Abstract

Breast cancer is the most common type of cancer among females and the adenosine triphosphate (ATP) binding cassette E1 (ABCE1) gene is a member of the ATP‑binding cassette (ABC) family. Studies in lung cancer have shown that overexpression of ABCE1 in tumor cells promotes growth and inhibits apoptosis. However, little is known about whether the ABCE1 gene is associated with breast cancer. In the present study, ABCE1 expression was assessed in breast cancer tissue and adjacent normal breast tissue using immunohistochemistry. Furthermore, small interfering (si)RNA targeting ABCE1 was constructed and transfected into MCF‑7 human breast cancer cells to downregulate ABCE1 expression. The effect of ABCE1 knockdown on cell proliferation, invasion, apoptosis and gene expression was then assessed using MTT assay, Transwell migration assay, flow cytometry and western blot analysis, respectively. ABCE1 was observed to be overexpressed in breast cancer tissue compared with adjacent normal breast tissue. Furthermore, ABCE1‑siRNA was found to inhibit proliferation and invasion in breast cancer cells, significantly induce breast cancer cell apoptosis (P<0.05) in vitro and increase the protein expression of RNase L. These findings showed that ABCE1 had an important role in proliferation, invasion and apoptosis in MCF‑7 human breast cancer cells and that ABCE1 may inhibit intracellular RNase L activity, which inhibits the 2‑5A/RNase L pathway, interfering with the biological characteristics of breast cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Huang B, Zhou H, Lang X and Liu Z: siRNA‑induced ABCE1 silencing inhibits proliferation and invasion of breast cancer cells. Mol Med Rep 10: 1685-1690, 2014.
APA
Huang, B., Zhou, H., Lang, X., & Liu, Z. (2014). siRNA‑induced ABCE1 silencing inhibits proliferation and invasion of breast cancer cells. Molecular Medicine Reports, 10, 1685-1690. https://doi.org/10.3892/mmr.2014.2424
MLA
Huang, B., Zhou, H., Lang, X., Liu, Z."siRNA‑induced ABCE1 silencing inhibits proliferation and invasion of breast cancer cells". Molecular Medicine Reports 10.4 (2014): 1685-1690.
Chicago
Huang, B., Zhou, H., Lang, X., Liu, Z."siRNA‑induced ABCE1 silencing inhibits proliferation and invasion of breast cancer cells". Molecular Medicine Reports 10, no. 4 (2014): 1685-1690. https://doi.org/10.3892/mmr.2014.2424
Copy and paste a formatted citation
x
Spandidos Publications style
Huang B, Zhou H, Lang X and Liu Z: siRNA‑induced ABCE1 silencing inhibits proliferation and invasion of breast cancer cells. Mol Med Rep 10: 1685-1690, 2014.
APA
Huang, B., Zhou, H., Lang, X., & Liu, Z. (2014). siRNA‑induced ABCE1 silencing inhibits proliferation and invasion of breast cancer cells. Molecular Medicine Reports, 10, 1685-1690. https://doi.org/10.3892/mmr.2014.2424
MLA
Huang, B., Zhou, H., Lang, X., Liu, Z."siRNA‑induced ABCE1 silencing inhibits proliferation and invasion of breast cancer cells". Molecular Medicine Reports 10.4 (2014): 1685-1690.
Chicago
Huang, B., Zhou, H., Lang, X., Liu, Z."siRNA‑induced ABCE1 silencing inhibits proliferation and invasion of breast cancer cells". Molecular Medicine Reports 10, no. 4 (2014): 1685-1690. https://doi.org/10.3892/mmr.2014.2424
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