Selenium treatment significantly inhibits tumor necrosis factor‑α‑induced cell death and tau hyperphosphorylation in neuroblastoma cells

Lee,Young  Ju; Kim,Ji  Eun; Kwak,Moon  Hwa; Go,Jun; Yang,Seung  Yun; Kwon,Hyeog  Soong; Kim,Byoung  Chul; Kim,Joo  Man; Hwang,Dae  Youn

doi:10.3892/mmr.2014.2442

October 2014 Volume 10 Issue 4

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October 2014 Volume 10 Issue 4

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Article

Selenium treatment significantly inhibits tumor necrosis factor‑α‑induced cell death and tau hyperphosphorylation in neuroblastoma cells

Authors:
- Young Ju Lee
- Ji Eun Kim
- Moon Hwa Kwak
- Jun Go
- Seung Yun Yang
- Hyeog Soong Kwon
- Byoung Chul Kim
- Joo Man Kim
- Dae Youn Hwang
View Affiliations / Copyright

Affiliations: College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang‑si, Gyeongsangnam‑do 627‑706, Republic of Korea
Pages: 1869-1874
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Published online on: August 4, 2014

https://doi.org/10.3892/mmr.2014.2442
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Abstract

The hyperphosphorylation of the protein tau disrupts its normal function on regulating axonal transport and leads to the accumulation of neurofibrillary tangles (NFT), which are involved in the pathogenesis of Alzheimer's disease (AD). This study was performed to investigate whether sodium selenite may inhibit the hyperphosphorylation of tau induced by treatment with tumor necrosis factor‑α (TNF‑α). For this purpose, we studied the changes in cell viability, tau phosphorylation and activity of tau kinases in TNF‑α+selenite-treated neuroblastoma cells. Cell viability was significantly recovered in the group cotreated with TNF‑α and 5 µM selenite for 24 h, but not in the groups treated with TNF‑α and lower concentrations of selenite. Tau phosphorylation was significantly higher in the group treated with TNF‑α+vehicle (instead of selenite) compared to the non‑treated group. However, in the TNF‑α+selenite‑treated group, the total phosphorylation level of tau protein at the Ser404 site was significantly reduced compared to the TNF‑α+vehicle group, although western blot analysis revealed one band of increased intensity in the p‑tau sample, corresponding to a phosphorylated tau isoform of 65‑70 kDa. In addition, sodium selenite treatment led to a significant recovery in the immunofluorescence intensity of the p‑tau protein in the cytoplasm and nucleus and in the apoptotic rate of neuroblastoma cells stained with the p‑tau antibody and 4',6‑diamidino‑2‑phenylindole (DAPI). The phosphorylation of two protein kinases responsible for phosphorylation of tau, glycogen synthase kinase 3β (GSK‑3β) and Akt, also known as protein kinase B, was markedly decreased in the TNF‑α+selenite‑treated group relative to the TNF‑α+vehicle‑treated group. Overall, these results provide strong evidence that sodium selenite (selenium) can inhibit cell death and tau phosphorylation induced by TNF‑α in neuroblastoma cells, through the inhibition GSK‑3β and Akt phosphorylation.

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1	Hanisch UK: Microglia as a source and target of cytokines. Glia. 40:140–155. 2002.PubMed/NCBI
2	Klintworth H, Garden G and Xia Z: Rotenone and paraquat do not directly activate microglia or induce inflammatory cytokine release. Neurosci Lett. 462:1–5. 2009. View Article : Google Scholar : PubMed/NCBI
3	Montgomery SL and Bowers WJ: Tumor necrosis factor-alpha and the roles it plays in homeostatic and degenerative processes within the central nervous system. J Neuroimmune Pharmacol. 7:42–59. 2012. View Article : Google Scholar
4	Rubio-Perez JM and Morillas-Ruiz JM: A review: inflammatory process in Alzheimer’s disease, role of cytokines. ScientificWorldJournal. 2012:7563572012.
5	Breder CD, Tsujimoto M, Terano Y, Scott DW and Saper CB: Distribution and characterization of tumor necrosis factor-α-like immunoreactivity in the murine central nervous system. J Comp Neurol. 337:543–567. 1993.
6	Swardfager W, Lanctot K, Rothenburg L, Wong A, Cappell J and Herrmann N: A meta-analysis of cytokines in Alzheimer’s disease. Biol Psychiatry. 68:930–941. 2010.
7	Dowlati Y, Herrmann N, Swardfager W, Liu H, Sham L, Reim EK and Lanctot KL: A meta-analysis of cytokines in major depression. Biol Psychiatry. 67:446–457. 2010. View Article : Google Scholar : PubMed/NCBI
8	Locksley RM, Killeen N and Lenardo MJ: The TNF and TNF receptor superfamilies: integrating mammalian biology. Cell. 104:487–501. 2001. View Article : Google Scholar : PubMed/NCBI
9	Brynskov J, Foegh P, Pedersen G, Ellervik C, Kirkegaard T, Bingham A and Saermark T: Tumour necrosis factor alpha converting enzyme (TACE) activity in the colonic mucosa of patients with inflammatory bowel disease. Gut. 51:37–43. 2002. View Article : Google Scholar : PubMed/NCBI
10	Wilber CG: Toxicology of selenium: a review. Clin Toxicol. 17:171–230. 1980. View Article : Google Scholar : PubMed/NCBI
11	van Eersel J, Ke YD, Liu X, Delerue F, Kril JJ, Gotz J and Ittner LM: Sodium selenate mitigates tau pathology, neurodegeneration, and functional deficits in Alzheimer’s disease models. Proc Natl Acad Sci USA. 107:13888–13893. 2010.PubMed/NCBI
12	Corcoran NM, Martin D, Hutter-Paier B, Windisch M, Nguyen T, Nheu L, Sundstrom LE, Costello AJ and Hovens CM: Sodium selenate specifically activates PP2A phosphatase, dephosphorylates tau and reverses memory deficits in an Alzheimer’s disease model. J Clin Neurosci. 17:1025–1033. 2010.PubMed/NCBI
13	Haratake M, Yoshida S, Mandai M, Fuchigami T and Nakayama M: Elevated amyloid-β plaque deposition in dietary selenium-deficient Tg2576 transgenic mice. Metallomics. 5:479–483. 2013.
14	Sarraf CE: Tumor necrosis factor and cell death in tumors (Review). Int J Oncol. 5:1333–1339. 1994.PubMed/NCBI
15	Wang JZ, Xia YY, Grundke-Iqbal I and Iqbal K: Abnormal hyperphosphorylation of tau: sites, regulation, and molecular mechanism of neurofibrillary degeneration. J Alzheimers Dis. 33(Suppl 1): S123–139. 2013.PubMed/NCBI
16	Chung ES, Bok E, Sohn S, Lee YD, Baik HH and Jin BK: GT1b-induced neurotoxicity is mediated by the Akt/GSK-3/tau signaling pathway but not caspase-3 in mesencephalic dopaminergic neurons. BMC Neurosci. 11:742010. View Article : Google Scholar
17	Food and Agriculture Organization of the United Nations (FAO)/World Health Organization (WHO). Human vitamin and mineral requirements: report of a Joint FAO/WHO expert consultation Bangkok, Thailand. FAO; Rome, Italy: pp. 235–255. 2002
18	Yang G and Zhou R: Further observations on the human maximum safe dietary selenium intake in a seleniferous area of China. J Trace Elem Electrolytes Health Dis. 8:159–165. 1994.PubMed/NCBI
19	Nuttall KL: Evaluating selenium poisoning. Ann Clin Lab Sci. 36:409–420. 2006.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Selenium treatment significantly inhibits tumor necrosis factor‑α‑induced cell death and tau hyperphosphorylation in neuroblastoma cells

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