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Article

Continuous hypoxia regulates the osteogenic potential of mesenchymal stem cells in a time-dependent manner

  • Authors:
    • Hao Ding
    • Song Chen
    • Jun-Hui Yin
    • Xue-Tao Xie
    • Zhen‑Hong  Zhu
    • You-Shui Gao
    • Chang-Qing Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, P.R. China
  • Pages: 2184-2190
    |
    Published online on: August 5, 2014
       https://doi.org/10.3892/mmr.2014.2451
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Abstract

The effects of hypoxia on the osteogenic potential of mesenchymal stem cells (MSCs) have been previously reported. From these studies, possible factors affecting the association between hypoxia and the osteogenic differentiation of MSCs have been suggested, including hypoxia severity, cell origin and methods of induction. The effect of the duration of hypoxia, however, remains poorly understood. The aim of the present study was to investigate the effect of continuous hypoxia on the induced osteogenesis of MSCs. Rat MSCs were isolated and cultured in vitro. Once the cells had been cultured to passage three, they were switched to 1% oxygen and cultured either with or without osteogenic medium, while cells in the control groups were cultured under normoxia in corresponding conditions. Four osteogenic differentiation biomarkers, runt‑related transcription factor 2, osteopontin, osteocalcin and alkaline phosphatase, were analyzed by quantitative polymerase chain reaction and western blotting at defined intervals throughout the culture period. In addition, Alizarin Red staining was used to assess changes in mineralization. The results showed that 1% hypoxia was able to enhance and accelerate the osteogenic ability of the MSCs during the initial phases of differentiation, and the protein expression of certain associated biomarkers was upregulated. However, continuous hypoxia was shown to impair osteogenesis in the latter stages of differentiation. These findings suggest that hypoxia can regulate the osteogenesis of MSCs in a time‑dependent manner.
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Copy and paste a formatted citation
Spandidos Publications style
Ding H, Chen S, Yin J, Xie X, Zhu ZH, Gao Y and Zhang C: Continuous hypoxia regulates the osteogenic potential of mesenchymal stem cells in a time-dependent manner. Mol Med Rep 10: 2184-2190, 2014.
APA
Ding, H., Chen, S., Yin, J., Xie, X., Zhu, Z., Gao, Y., & Zhang, C. (2014). Continuous hypoxia regulates the osteogenic potential of mesenchymal stem cells in a time-dependent manner. Molecular Medicine Reports, 10, 2184-2190. https://doi.org/10.3892/mmr.2014.2451
MLA
Ding, H., Chen, S., Yin, J., Xie, X., Zhu, Z., Gao, Y., Zhang, C."Continuous hypoxia regulates the osteogenic potential of mesenchymal stem cells in a time-dependent manner". Molecular Medicine Reports 10.4 (2014): 2184-2190.
Chicago
Ding, H., Chen, S., Yin, J., Xie, X., Zhu, Z., Gao, Y., Zhang, C."Continuous hypoxia regulates the osteogenic potential of mesenchymal stem cells in a time-dependent manner". Molecular Medicine Reports 10, no. 4 (2014): 2184-2190. https://doi.org/10.3892/mmr.2014.2451
Copy and paste a formatted citation
x
Spandidos Publications style
Ding H, Chen S, Yin J, Xie X, Zhu ZH, Gao Y and Zhang C: Continuous hypoxia regulates the osteogenic potential of mesenchymal stem cells in a time-dependent manner. Mol Med Rep 10: 2184-2190, 2014.
APA
Ding, H., Chen, S., Yin, J., Xie, X., Zhu, Z., Gao, Y., & Zhang, C. (2014). Continuous hypoxia regulates the osteogenic potential of mesenchymal stem cells in a time-dependent manner. Molecular Medicine Reports, 10, 2184-2190. https://doi.org/10.3892/mmr.2014.2451
MLA
Ding, H., Chen, S., Yin, J., Xie, X., Zhu, Z., Gao, Y., Zhang, C."Continuous hypoxia regulates the osteogenic potential of mesenchymal stem cells in a time-dependent manner". Molecular Medicine Reports 10.4 (2014): 2184-2190.
Chicago
Ding, H., Chen, S., Yin, J., Xie, X., Zhu, Z., Gao, Y., Zhang, C."Continuous hypoxia regulates the osteogenic potential of mesenchymal stem cells in a time-dependent manner". Molecular Medicine Reports 10, no. 4 (2014): 2184-2190. https://doi.org/10.3892/mmr.2014.2451
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