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Article

Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF‑β signaling pathway

  • Authors:
    • Fenglin Chen
    • Mingkai Zhuang
    • Jun Peng
    • Xiaozhong Wang
    • Tingxuan Huang
    • Sanmei Li
    • Manqiang Lin
    • Hongming Lin
    • Yating Xu
    • Jianying Li
    • Zhixin Chen
    • Yuehong Huang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Union Hospital of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China, College of Union Clinical Medicine, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China, Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350001, P.R. China, College of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China
  • Pages: 1999-2003
    |
    Published online on: August 5, 2014
       https://doi.org/10.3892/mmr.2014.2452
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Abstract

The transforming growth factor‑β (TGF‑β) signaling pathway exhibits an important role in cancer invasion and metastasis. Excessive expression of TGF‑β activates Smad4, leading to the upregulation of downstream metastasis-associated genes. Thus, the inhibition of the TGF‑β/Smad4 signaling pathway may be a novel strategy for treatment of cancer metastasis. Baicalein, a flavonoid derived from the root of Scutellaria baicalensis, has been reported to exert strong anti‑tumor activity towards various types of cancer. In the present study the effect of baicalein on migration and invasion of cancer cells was evaluated using wound‑healing and Transwell assays. In order to investigate the possible molecular mechanisms of the anti‑metastatic effects of baicalein, quantitative polymerase chain reaction (qPCR) and western blot analyses were performed to examine the effect on the expression of TGF‑β, Smad4, N‑cadherin, vimentin, ZEB1 and ZEB2. It was determined that baicalein inhibited the migration and invasion of AGS cells by suppressing the TGF‑β/Smad4 signaling pathway. In addition, baicalein treatment reduced the expression of the metastasis‑associated N‑cadherin, vimentin, ZEB1 and ZEB2, downstream target genes of the TGF‑β/Smad4 signaling pathway. Collectively, these results suggest that inhibition of the metastasis of cancer cells via inactivation of TGF‑β/Smad4 signaling is one of the mechanisms by which baicalein may treat cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Chen F, Zhuang M, Peng J, Wang X, Huang T, Li S, Lin M, Lin H, Xu Y, Li J, Li J, et al: Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF‑β signaling pathway. Mol Med Rep 10: 1999-2003, 2014.
APA
Chen, F., Zhuang, M., Peng, J., Wang, X., Huang, T., Li, S. ... Huang, Y. (2014). Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF‑β signaling pathway. Molecular Medicine Reports, 10, 1999-2003. https://doi.org/10.3892/mmr.2014.2452
MLA
Chen, F., Zhuang, M., Peng, J., Wang, X., Huang, T., Li, S., Lin, M., Lin, H., Xu, Y., Li, J., Chen, Z., Huang, Y."Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF‑β signaling pathway". Molecular Medicine Reports 10.4 (2014): 1999-2003.
Chicago
Chen, F., Zhuang, M., Peng, J., Wang, X., Huang, T., Li, S., Lin, M., Lin, H., Xu, Y., Li, J., Chen, Z., Huang, Y."Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF‑β signaling pathway". Molecular Medicine Reports 10, no. 4 (2014): 1999-2003. https://doi.org/10.3892/mmr.2014.2452
Copy and paste a formatted citation
x
Spandidos Publications style
Chen F, Zhuang M, Peng J, Wang X, Huang T, Li S, Lin M, Lin H, Xu Y, Li J, Li J, et al: Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF‑β signaling pathway. Mol Med Rep 10: 1999-2003, 2014.
APA
Chen, F., Zhuang, M., Peng, J., Wang, X., Huang, T., Li, S. ... Huang, Y. (2014). Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF‑β signaling pathway. Molecular Medicine Reports, 10, 1999-2003. https://doi.org/10.3892/mmr.2014.2452
MLA
Chen, F., Zhuang, M., Peng, J., Wang, X., Huang, T., Li, S., Lin, M., Lin, H., Xu, Y., Li, J., Chen, Z., Huang, Y."Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF‑β signaling pathway". Molecular Medicine Reports 10.4 (2014): 1999-2003.
Chicago
Chen, F., Zhuang, M., Peng, J., Wang, X., Huang, T., Li, S., Lin, M., Lin, H., Xu, Y., Li, J., Chen, Z., Huang, Y."Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF‑β signaling pathway". Molecular Medicine Reports 10, no. 4 (2014): 1999-2003. https://doi.org/10.3892/mmr.2014.2452
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