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Article

CCAAT/enhancer‑binding protein β regulates interleukin‑6‑induced transmembrane and ubiquitin‑like domain containing 1 gene expression in hepatocytes

  • Authors:
    • Menggang Liu
    • Tao Yuan
    • Hongming Liu
    • Ping Chen
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Daping Hospital, The Third Military Medical University, Chongqing 400042, P.R. China
  • Pages: 2177-2183
    |
    Published online on: August 6, 2014
       https://doi.org/10.3892/mmr.2014.2457
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Abstract

Transmembrane and ubiquitin‑like domain containing 1 (Tmub1) protein has a negative effect on liver regeneration; however, the mechanisms of Tmub1 expression regulation are currently unknown. CCAAT/enhancer‑binding protein β (C/EBPβ) is a key transcription factor associated with interleukin‑6 (IL‑6) in liver regeneration. The present study aimed to investigate the involvement of C/EBPβ in the IL‑6‑induced upregulation of Tmub1. A series of Tmub1 luciferase reporter vectors were constructed to detect the effects of IL‑6 on the transcriptional activity of Tmub1. Small interfering RNA and overexpression vectors were employed to either inhibit or enhance the expression of C/EBPβ, respectively, to determine the effect of C/EBPβ on Tmub1 expression in liver cells. Additionally, chromatin immunoprecipitation experiments were performed to determine whether C/EBPβ bound to the Tmub1 gene, and the sequences associated with the regulation of Tmub1 expression by C/EBPβ were subsequently determined. Several potential binding sites of C/EBPβ were identified in the 5'‑upstream region of the Tmub1 gene. C/EBPβ expression was positively correlated with Tmub1 gene expression in rat liver cells. Furthermore, it was demonstrated that C/EBPβ bound to 5'‑upstream sequences of the Tmub1 gene to enhance the promoter activity. The present study demonstrated that C/EBPβ is a key transcription factor, which can positively regulate the expression of Tmub1 during liver cell proliferation through a possible association with IL‑6.
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1 

Duncan AW and Soto-Gutierrez A: Liver repopulation and regeneration: new approaches to old questions. Curr Opin Organ Transplant. 18:197–202. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Kurinna S and Barton MC: Cascades of transcription regulation during liver regeneration. Int J Biochem Cell Biol. 43:189–197. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Michalopoulos GK and DeFrances MC: Liver regeneration. Science. 276:60–66. 1997. View Article : Google Scholar

4 

Stolz DB, Mars WM, Petersen BE, Kim TH and Michalopoulos GK: Growth factor signal transduction immediately after two-thirds partial hepatectomy in the rat. Cancer Res. 59:3954–3960. 1999.PubMed/NCBI

5 

Fausto N: Liver regeneration. J Hepatol. 32:19–31. 2000. View Article : Google Scholar

6 

Kountouras J, Boura P and Lygidakis NJ: Liver regeneration after hepatectomy. Hepatogastroenterology. 48:556–562. 2001.

7 

Pahlavan PS, Feldmann RE Jr, Zavos C and Kountouras J: Prometheus’ challenge: molecular, cellular and systemic aspects of liver regeneration. J Surg Res. 134:238–251. 2006.

8 

Streetz KL, Luedde T, Manns MP and Trautwein C: Interleukin 6 and liver regeneration. Gut. 47:309–312. 2000. View Article : Google Scholar

9 

Schumann RR, Kirschning CJ, Unbehaun A, Aberle HP, Knope HP, Lamping N, Ulevitch RJ and Herrmann F: The lipopolysaccharide-binding protein is a secretory class 1 acute-phase protein whose gene is transcriptionally activated by APRF/STAT/3 and other cytokine-inducible nuclear proteins. Mol Cell Biol. 16:3490–3503. 1996.

10 

Brown RT, Ades IZ and Nordan RP: An acute phase response factor/NF-kappa B site downstream of the junB gene that mediates responsiveness to interleukin-6 in a murine plasmacytoma. J Biol Chem. 270:31129–31135. 1995. View Article : Google Scholar : PubMed/NCBI

11 

Osada S, Yamamoto H, Nishihara T and Imagawa M: DNA binding specificity of the CCAAT/enhancer-binding protein transcription factor family. J Biol Chem. 271:3891–3896. 1996. View Article : Google Scholar

12 

Takiguchi M: The C/EBP family of transcription factors in the liver and other organs. Int J Exp Pathol. 79:369–391. 1998. View Article : Google Scholar : PubMed/NCBI

13 

Greenbaum LE, Li W, Cressman DE, Peng Y, Ciliberto G, Poli V and Taub R: CCAAT enhancer-binding protein beta is required for normal hepatocyte proliferation in mice after partial hepatectomy. J Clin Invest. 102:996–1007. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Akira S, Isshiki H, Sugita T, Tanabe O, Kinoshita S, Nishio Y, Nakajima T, Hirano T and Kishimoto T: A nuclear factor for IL-6 expression (NF-IL6) is a member of a C/EBP family. EMBO J. 9:1897–1906. 1990.PubMed/NCBI

15 

Akira S and Kishimoto T: IL-6 and NF-IL6 in acute-phase response and viral infection. Immunol Rev. 127:25–50. 1992. View Article : Google Scholar : PubMed/NCBI

16 

Akira S: IL-6-regulated transcription factors. Int J Biochem Cell Biol. 29:1401–1418. 1997. View Article : Google Scholar

17 

Mayer C, Gruber HJ, Landl EM, Pailer S, Scharnagl H, Truschnig-Wilders M and März W: Rosuvastatin reduces interleukin-6-induced expression of C-reactive protein in human hepatocytes in a STAT3- and C/EBP-dependent fashion. Int J Clin Pharmacol Ther. 45:319–327. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Della Fazia MA, Castelli M, Bartoli D, Pieroni S, Pettirossi V, Piobbico D, Viola-Magni M and Servillo G: HOPS: a novel cAMP-dependent shuttling protein involved in protein synthesis regulation. J Cell Sci. 118:3185–3194. 2005.PubMed/NCBI

19 

Liu M, Liu H, Wang X, Chen P and Chen H: IL-6-induction of hepatocyte proliferation through the Tmub1-regulated gene pathway. Int J Mol Med. 29:1106–1112. 2012.PubMed/NCBI

20 

Flodby P, Antonson P, Barlow C, Blanck A, Porsch-Hällström I and Xanthopoulos KG: Differential patterns of expression of three C/EBP isoforms, HNF-1, and HNF-4 after partial hepatectomy in rats. Exp Cell Res. 208:248–256. 1993. View Article : Google Scholar : PubMed/NCBI

21 

Cressmann DE, Diamond RH and Taub R: Rapid activation of the Stat3 transcription complex in liver regeneration. Hepatology. 21:1443–1449. 1995. View Article : Google Scholar : PubMed/NCBI

22 

Niehof M, Streetz K, Rakemann T, Bischoff SC, Manns MP, Horn F and Trautwein C: Interleukin-6-induced tethering of STAT3 to the LAP/C/EBPbeta promoter suggests a new mechanism of transcriptional regulation by STAT3. J Biol Chem. 276:9016–9127. 2001. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Liu M, Yuan T, Liu H and Chen P: CCAAT/enhancer‑binding protein β regulates interleukin‑6‑induced transmembrane and ubiquitin‑like domain containing 1 gene expression in hepatocytes. Mol Med Rep 10: 2177-2183, 2014.
APA
Liu, M., Yuan, T., Liu, H., & Chen, P. (2014). CCAAT/enhancer‑binding protein β regulates interleukin‑6‑induced transmembrane and ubiquitin‑like domain containing 1 gene expression in hepatocytes. Molecular Medicine Reports, 10, 2177-2183. https://doi.org/10.3892/mmr.2014.2457
MLA
Liu, M., Yuan, T., Liu, H., Chen, P."CCAAT/enhancer‑binding protein β regulates interleukin‑6‑induced transmembrane and ubiquitin‑like domain containing 1 gene expression in hepatocytes". Molecular Medicine Reports 10.4 (2014): 2177-2183.
Chicago
Liu, M., Yuan, T., Liu, H., Chen, P."CCAAT/enhancer‑binding protein β regulates interleukin‑6‑induced transmembrane and ubiquitin‑like domain containing 1 gene expression in hepatocytes". Molecular Medicine Reports 10, no. 4 (2014): 2177-2183. https://doi.org/10.3892/mmr.2014.2457
Copy and paste a formatted citation
x
Spandidos Publications style
Liu M, Yuan T, Liu H and Chen P: CCAAT/enhancer‑binding protein β regulates interleukin‑6‑induced transmembrane and ubiquitin‑like domain containing 1 gene expression in hepatocytes. Mol Med Rep 10: 2177-2183, 2014.
APA
Liu, M., Yuan, T., Liu, H., & Chen, P. (2014). CCAAT/enhancer‑binding protein β regulates interleukin‑6‑induced transmembrane and ubiquitin‑like domain containing 1 gene expression in hepatocytes. Molecular Medicine Reports, 10, 2177-2183. https://doi.org/10.3892/mmr.2014.2457
MLA
Liu, M., Yuan, T., Liu, H., Chen, P."CCAAT/enhancer‑binding protein β regulates interleukin‑6‑induced transmembrane and ubiquitin‑like domain containing 1 gene expression in hepatocytes". Molecular Medicine Reports 10.4 (2014): 2177-2183.
Chicago
Liu, M., Yuan, T., Liu, H., Chen, P."CCAAT/enhancer‑binding protein β regulates interleukin‑6‑induced transmembrane and ubiquitin‑like domain containing 1 gene expression in hepatocytes". Molecular Medicine Reports 10, no. 4 (2014): 2177-2183. https://doi.org/10.3892/mmr.2014.2457
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