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Article

Protective effect of proanthocyanidins on anoxia-reoxygenation injury of myocardial cells mediated by the PI3K/Akt/GSK-3β pathway and mitochondrial ATP-sensitive potassium channel

  • Authors:
    • Yanyan Hu
    • Li Li
    • Wenbin Yin
    • Lin Shen
    • Beian You
    • Haiqing Gao
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China
  • Pages: 2051-2058
    |
    Published online on: August 6, 2014
       https://doi.org/10.3892/mmr.2014.2459
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Abstract

The aim of the present study was to examine the protective effect of proanthocyanidins anoxia-reoxygenation injury of myocardial cells and its association with phosphatidylinositol-3-kinase/Akt and glycogen synthase kinase (PI3K/Akt/GSK)-3β and ATP-sensitive potassium channels. Neonatal rat myocardial cells were cultured and an anoxia-reoxygenation model was established following pretreatment with various drugs. The experiment was divided into five groups according to an experimental scheme. An MTT assay was used to examine the cell survival, and reactive oxygen species (ROS) levels and apoptosis were detected by flow cytometry. Myocardial apoptosis was also examined by terminal deoxynucleotidyl transferase dUTP nick end labeling staining and western blot analysis was employed to detect the expression levels of caspase-3, p-Akt and p-glycogen synthase kinase (GSK)-3β. The results revealed that myocardial cells in the anoxia-reoxygenation group (A/R) exhibited reduced survival rates, increased ROS levels and enhanced caspase-3 expression, as compared with the control group (CN; P<0.05). However, the increase in p-Akt and p-GSK-3β expression was not significantly different. In the proanthocyanidin pretreatment group (PC) the myocardial cell survival rate was increased, ROS levels were reduced, caspase-3 expression was decreased and p-Akt and p-GSK-3β expression levels were significantly increased as compared with the A/R group (P<0.05). Blockade of the PIK3/Akt channel by LY294002 eliminated the protective effects of proanthocyanidins and induced a significant decrease in p-Akt protein and p-GSK-3β expression levels as compared with the PC group. The inhibitor of mitochondrial ATP-sensitive potassium (mitoKATP) channels, 5-HD, also significantly suppressed the protective effects of proanthocyanidins, but had no evident impact on p-Akt and p-GSK-3β expression as compared with the PC group. In conclusion, pretreatment with proanthocyanidins had a protective effect on rat myocardial cell anoxia/reoxygenation injury. This effect is associated with the activation of the PI3K/Akt/GSK-3β signaling pathway and the opening of mitoKATP channels, which may have important roles downstream of PI3K.
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Copy and paste a formatted citation
Spandidos Publications style
Hu Y, Li L, Yin W, Shen L, You B and Gao H: Protective effect of proanthocyanidins on anoxia-reoxygenation injury of myocardial cells mediated by the PI3K/Akt/GSK-3β pathway and mitochondrial ATP-sensitive potassium channel. Mol Med Rep 10: 2051-2058, 2014.
APA
Hu, Y., Li, L., Yin, W., Shen, L., You, B., & Gao, H. (2014). Protective effect of proanthocyanidins on anoxia-reoxygenation injury of myocardial cells mediated by the PI3K/Akt/GSK-3β pathway and mitochondrial ATP-sensitive potassium channel. Molecular Medicine Reports, 10, 2051-2058. https://doi.org/10.3892/mmr.2014.2459
MLA
Hu, Y., Li, L., Yin, W., Shen, L., You, B., Gao, H."Protective effect of proanthocyanidins on anoxia-reoxygenation injury of myocardial cells mediated by the PI3K/Akt/GSK-3β pathway and mitochondrial ATP-sensitive potassium channel". Molecular Medicine Reports 10.4 (2014): 2051-2058.
Chicago
Hu, Y., Li, L., Yin, W., Shen, L., You, B., Gao, H."Protective effect of proanthocyanidins on anoxia-reoxygenation injury of myocardial cells mediated by the PI3K/Akt/GSK-3β pathway and mitochondrial ATP-sensitive potassium channel". Molecular Medicine Reports 10, no. 4 (2014): 2051-2058. https://doi.org/10.3892/mmr.2014.2459
Copy and paste a formatted citation
x
Spandidos Publications style
Hu Y, Li L, Yin W, Shen L, You B and Gao H: Protective effect of proanthocyanidins on anoxia-reoxygenation injury of myocardial cells mediated by the PI3K/Akt/GSK-3β pathway and mitochondrial ATP-sensitive potassium channel. Mol Med Rep 10: 2051-2058, 2014.
APA
Hu, Y., Li, L., Yin, W., Shen, L., You, B., & Gao, H. (2014). Protective effect of proanthocyanidins on anoxia-reoxygenation injury of myocardial cells mediated by the PI3K/Akt/GSK-3β pathway and mitochondrial ATP-sensitive potassium channel. Molecular Medicine Reports, 10, 2051-2058. https://doi.org/10.3892/mmr.2014.2459
MLA
Hu, Y., Li, L., Yin, W., Shen, L., You, B., Gao, H."Protective effect of proanthocyanidins on anoxia-reoxygenation injury of myocardial cells mediated by the PI3K/Akt/GSK-3β pathway and mitochondrial ATP-sensitive potassium channel". Molecular Medicine Reports 10.4 (2014): 2051-2058.
Chicago
Hu, Y., Li, L., Yin, W., Shen, L., You, B., Gao, H."Protective effect of proanthocyanidins on anoxia-reoxygenation injury of myocardial cells mediated by the PI3K/Akt/GSK-3β pathway and mitochondrial ATP-sensitive potassium channel". Molecular Medicine Reports 10, no. 4 (2014): 2051-2058. https://doi.org/10.3892/mmr.2014.2459
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