Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
October 2014 Volume 10 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October 2014 Volume 10 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Mass spectrometry-based, label-free quantitative proteomics of round spermatids in mice

  • Authors:
    • Hailong Wang
    • Yan Li
    • Lijuan Yang
    • Baofeng Yu
    • Ping Yan
    • Min Pang
    • Xiaobing Li
    • Hong Yang
    • Guoping Zheng
    • Jun Xie
    • Rui Guo
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China , Fan-Xing Biological Technology Co., Ltd., Beijing 010000, P.R. China, Respiratory Department, The First Affiliated Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2009-2024
    |
    Published online on: August 6, 2014
       https://doi.org/10.3892/mmr.2014.2460
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Round haploid spermatids are formed at the completion of meiosis. These spermatids then undergo morphological and cytological changes during spermiogenesis. Although sperm proteomes have been extensively studied, relatively few studies have specifically investigated the proteome of round spermatids. We developed a label-free quantitative method in combination with 2D-nano-LC-ESI-MS/MS to investigate the proteome of round spermatids in mice. Analysis of the proteomic data identified 2,331 proteins in the round spermatids. Functional classification of the proteins based on Gene Ontology terms and enrichment analysis further revealed the following: 504 of the identified proteins are predicted to be involved in the generation of precursor metabolites and energy; 343 proteins in translation and protein targeting; 298 proteins in nucleotide and nucleic acid metabolism; 275 and 289 proteins in transport and cellular component organization, respectively. A number of the identified proteins were associated with cytoskeleton organization (183), protein degradation (116) and response to stimulus (115). KEGG pathway analysis identified 68 proteins that are annotated as components of the ribosomal pathway and 17 proteins were related to aminoacyl-tRNA biosynthesis. The round spermatids also contained 28 proteins involved in the proteasome pathway and 40 proteins in the lysosome pathway. A total of 60 proteins were annotated as parts of the spliceosome pathway, in which heterogeneous nuclear RNA is converted to mRNA. Approximately 94 proteins were identified as actin‑binding proteins, involved in the regulation of the actin cytoskeleton. In conclusion, using a label-free shotgun proteomic approach, we identified numerous proteins associated with spermiogenesis in round spermatids.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Blanco-Rodriguez J and Martinez-Garcia C: Spontaneous germ cell death in the testis of the adult rat takes the form of apoptosis: re-evaluation of cell types that exhibit the ability to die during spermatogenesis. Cell Prolif. 29:13–31. 1996. View Article : Google Scholar

2 

Jan SZ, Hamer G, Repping S, de Rooij DG, van Pelt AM and Vormer TL: Molecular control of rodent spermatogenesis. Biochim Biophys Acta. 1822.1838–1850. 2012.PubMed/NCBI

3 

Anway MD, Li Y, Ravindranath N, Dym M and Griswold MD: Expression of testicular germ cell genes identified by differential display analysis. J Androl. 24:173–184. 2003.PubMed/NCBI

4 

O’Shaughnessy PJ, Fleming L, Baker PJ, Jackson G and Johnston H: Identification of developmentally regulated genes in the somatic cells of the mouse testis using serial analysis of gene expression. Biol Reprod. 69:797–808. 2003.

5 

Schlecht U, Demougin P, Koch R, et al: Expression profiling of mammalian male meiosis and gametogenesis identifies novel candidate genes for roles in the regulation of fertility. Mol Biol Cell. 15:1031–1043. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Kovac JR, Pastuszak AW and Lamb DJ: The use of genomics, proteomics, and metabolomics in identifying biomarkers of male infertility. Fertil Steril. 99:998–1007. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Baker MA, Hetherington L, Reeves GM and Aitken RJ: The mouse sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification. Proteomics. 8:1720–1730. 2008. View Article : Google Scholar

8 

Baker MA, Reeves G, Hetherington L and Aitken RJ: Analysis of proteomic changes associated with sperm capacitation through the combined use of IPG-strip pre-fractionation followed by RP chromatography LC-MS/MS analysis. Proteomics. 10:482–495. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Oliva R and Castillo J: Proteomics and the genetics of sperm chromatin condensation. Asian J Androl. 13:24–30. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Paz M, Morin M and Del Mazo J: Proteome profile changes during mouse testis development. Comp Biochem Physiol Part D Genomics Proteomics. 1:404–415. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Xun Z, Kaufman TC and Clemmer DE: Stable isotope labeling and label-free proteomics of Drosophila parkin null mutants. J Proteome Res. 8:4500–4510. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Cravatt BF, Simon GM and Yates JR III: The biological impact of mass-spectrometry-based proteomics. Nature. 450:991–1000. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Bauer KM, Lambert PA and Hummon AB: Comparative label-free LC-MS/MS analysis of colorectal adenocarcinoma and metastatic cells treated with 5-fluorouracil. Proteomics. 12:1928–1937. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Zhu W, Smith JW and Huang CM: Mass spectrometry-based label-free quantitative proteomics. J Biomed Biotechnol. 2010:8405182010.PubMed/NCBI

15 

Clough T, Thaminy S, Ragg S, Aebersold R and Vitek O: Statistical protein quantification and significance analysis in label-free LC-MS experiments with complex designs. BMC Bioinformatics. 13:S62012. View Article : Google Scholar : PubMed/NCBI

16 

Merl J, Ueffing M, Hauck SM and von Toerne C: Direct comparison of MS-based label-free and SILAC quantitative proteome profiling strategies in primary retinal Muller cells. Proteomics. 12:1902–1911. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Niehl A, Zhang ZJ, Kuiper M, Peck SC and Heinlein M: Label-free quantitative proteomic analysis of systemic responses to local wounding and virus infection in Arabidopsis thaliana. J Proteome Res. 12:2491–2503. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Bellve AR, Cavicchia JC, Millette CF, O’Brien DA, Bhatnagar YM and Dym M: Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization. J Cell Biol. 74:68–85. 1977. View Article : Google Scholar : PubMed/NCBI

19 

Keller A, Nesvizhskii AI, Kolker E and Aebersold R: Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search. Anal Chem. 74:5383–5392. 2002. View Article : Google Scholar

20 

Nesvizhskii AI, Keller A, Kolker E and Aebersold R: A statistical model for identifying proteins by tandem mass spectrometry. Anal Chem. 75:4646–4658. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Wilkins MR, Sanchez JC, Gooley AA, et al: Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it. Biotechnol Genet Eng Rev. 13:19–50. 1996. View Article : Google Scholar : PubMed/NCBI

22 

Wright PC, Noirel J, Ow SY and Fazeli A: A review of current proteomics technologies with a survey on their widespread use in reproductive biology investigations. Theriogenology. 77:738–765. 2012. View Article : Google Scholar

23 

Macleod G and Varmuza S: The application of proteomic approaches to the study of mammalian spermatogenesis and sperm function. FEBS J. 280:5635–5651. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Gardiner-Garden M, Ballesteros M, Gordon M and Tam PP: Histone- and protamine-DNA association: conservation of different patterns within the beta-globin domain in human sperm. Mol Cell Biol. 18:3350–3356. 1998.PubMed/NCBI

25 

Miki K: Energy metabolism and sperm function. Soc Reprod Fertil Suppl. 65:309–325. 2007.PubMed/NCBI

26 

Bajpai M, Gupta G and Setty BS: Changes in carbohydrate metabolism of testicular germ cells during meiosis in the rat. Eur J Endocrinol. 138:322–327. 1998. View Article : Google Scholar : PubMed/NCBI

27 

Nakamura M, Okinaga S and Arai K: Metabolism of round spermatids: kinetic properties of pyruvate kinase. Andrologia. 19:91–96. 1987. View Article : Google Scholar : PubMed/NCBI

28 

Groettrup M, Pelzer C, Schmidtke G and Hofmann K: Activating the ubiquitin family: UBA6 challenges the field. Trends Biochem Sci. 33:230–237. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Pelzer C and Groettrup M: FAT10: Activated by UBA6 and functioning in protein degradation. Subcell Biochem. 54:238–246. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Jin J, Li X, Gygi SP and Harper JW: Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature. 447:1135–1138. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Kopanja D, Roy N, Stoyanova T, et al: Cul4A is essential for spermatogenesis and male fertility. Dev Biol. 352:278–287. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Yi YJ, Manandhar G, Sutovsky M, et al: Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization. Biol Reprod. 77:780–793. 2007. View Article : Google Scholar

33 

Mtango NR, Sutovsky M, Susor A, Zhong Z, Latham KE and Sutovsky P: Essential role of maternal UCHL1 and UCHL3 in fertilization and preimplantation embryo development. J Cell Physiol. 227:1592–1603. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Tanaka H and Baba T: Gene expression in spermiogenesis. Cell Mol Life Sci. 62:344–354. 2005. View Article : Google Scholar

35 

Ito C, Yamatoya K, Yoshida K, et al: Integration of the mouse sperm fertilization-related protein equatorin into the acrosome during spermatogenesis as revealed by super-resolution and immunoelectron microscopy. Cell Tissue Res. 352:739–750. 2013. View Article : Google Scholar

36 

Dean KA, von Ahsen O, Gorlich D and Fried HM: Signal recognition particle protein 19 is imported into the nucleus by importin 8 (RanBP8) and transportin. J Cell Sci. 114:3479–3485. 2001.PubMed/NCBI

37 

van Nues RW, Leung E, McDonald JC, Dantuluru I and Brown JD: Roles for Srp72p in assembly, nuclear export and function of the signal recognition particle. RNA Biol. 5:73–83. 2008.PubMed/NCBI

38 

Sperry AO: The dynamic cytoskeleton of the developing male germ cell. Biol Cell. 104:297–305. 2012. View Article : Google Scholar : PubMed/NCBI

39 

Sun X, Kovacs T, Hu YJ and Yang WX: The role of actin and myosin during spermatogenesis. Mol Biol Rep. 38:3993–4001. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang H, Li Y, Yang L, Yu B, Yan P, Pang M, Li X, Yang H, Zheng G, Xie J, Xie J, et al: Mass spectrometry-based, label-free quantitative proteomics of round spermatids in mice. Mol Med Rep 10: 2009-2024, 2014.
APA
Wang, H., Li, Y., Yang, L., Yu, B., Yan, P., Pang, M. ... Guo, R. (2014). Mass spectrometry-based, label-free quantitative proteomics of round spermatids in mice. Molecular Medicine Reports, 10, 2009-2024. https://doi.org/10.3892/mmr.2014.2460
MLA
Wang, H., Li, Y., Yang, L., Yu, B., Yan, P., Pang, M., Li, X., Yang, H., Zheng, G., Xie, J., Guo, R."Mass spectrometry-based, label-free quantitative proteomics of round spermatids in mice". Molecular Medicine Reports 10.4 (2014): 2009-2024.
Chicago
Wang, H., Li, Y., Yang, L., Yu, B., Yan, P., Pang, M., Li, X., Yang, H., Zheng, G., Xie, J., Guo, R."Mass spectrometry-based, label-free quantitative proteomics of round spermatids in mice". Molecular Medicine Reports 10, no. 4 (2014): 2009-2024. https://doi.org/10.3892/mmr.2014.2460
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Li Y, Yang L, Yu B, Yan P, Pang M, Li X, Yang H, Zheng G, Xie J, Xie J, et al: Mass spectrometry-based, label-free quantitative proteomics of round spermatids in mice. Mol Med Rep 10: 2009-2024, 2014.
APA
Wang, H., Li, Y., Yang, L., Yu, B., Yan, P., Pang, M. ... Guo, R. (2014). Mass spectrometry-based, label-free quantitative proteomics of round spermatids in mice. Molecular Medicine Reports, 10, 2009-2024. https://doi.org/10.3892/mmr.2014.2460
MLA
Wang, H., Li, Y., Yang, L., Yu, B., Yan, P., Pang, M., Li, X., Yang, H., Zheng, G., Xie, J., Guo, R."Mass spectrometry-based, label-free quantitative proteomics of round spermatids in mice". Molecular Medicine Reports 10.4 (2014): 2009-2024.
Chicago
Wang, H., Li, Y., Yang, L., Yu, B., Yan, P., Pang, M., Li, X., Yang, H., Zheng, G., Xie, J., Guo, R."Mass spectrometry-based, label-free quantitative proteomics of round spermatids in mice". Molecular Medicine Reports 10, no. 4 (2014): 2009-2024. https://doi.org/10.3892/mmr.2014.2460
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team