Differential expression profiling of matrix metalloproteinases and tissue inhibitors of metalloproteinases in females 
with or without pelvic organ prolapse

Wang,Xiaohong; Li,Yiqi; Chen,Jie; Guo,Xiaoli; Guan,Han; Li,Candong

doi:10.3892/mmr.2014.2467

October 2014 Volume 10 Issue 4

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

October 2014 Volume 10 Issue 4

Full Size Image

Article

Differential expression profiling of matrix metalloproteinases and tissue inhibitors of metalloproteinases in females with or without pelvic organ prolapse

Authors:
- Xiaohong Wang
- Yiqi Li
- Jie Chen
- Xiaoli Guo
- Han Guan
- Candong Li
View Affiliations / Copyright

Affiliations: Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350004, P.R. China
Pages: 2004-2008
|
Published online on: August 8, 2014

https://doi.org/10.3892/mmr.2014.2467
Expand metrics +

Abstract

Pelvic organ prolapse (POP) is a common disorder that can disturb the health and quality of life of females. However, the basic pathophysiology and underlying mechanism of POP are not fully understood. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) have been reported to be associated with the onset and development of POP. In the present study, to characterize the differential expression profile of MMPs and TIMPs in female patients with and without POP, a total of 72 POP patients were sampled as a patient group and 72 non-POP patients that underwent hysterectomy due to benign tumors were sampled as a control group. Immunohistochemistry and polymerase chain reaction analysis were used to detect the expression levels of MMP-1, -2, -3 and -9 as well as TIMP-1 protein and mRNA in the anterior vaginal wall tissues. The expression levels of MMP-1, -2, -3 and -9 in the patient group were found to be significantly higher than those in the control group. By contrast, TIMP-1 expression levels in the patient group were significantly lower than those in the control group. Correlational analysis revealed a significantly positive correlation among the expression levels of MMP-2, -3 and -9. TIMP-1 expression levels were significantly negatively correlated with the expression levels of MMP-3 and -9. In addition, the expression levels of MMP-1 exhibited a positive correlation with those of MMP-2, -3 and -9, but a negative correlation with those of TIMP-1. The results demonstrated that the increased expression levels of MMPs and the reduced expression levels of TIMPs were directly associated with the presence of uterine prolapse, indicating that the differential expression levels of MMPs and TIMPs were correlated with the occurrence and development of POP. This data may assist in elucidating the molecular mechanism of MMP and TIMP involvement in POP, and also provide an underlying theoretical basis for the prevention and treatment of POP.

View Figures

View References

1	Weber AM and Richter HE: Pelvic organ prolapse. Obstet Gynecol. 106:615–634. 2005. View Article : Google Scholar : PubMed/NCBI
2	Goh JT: Biomechanical and biochemical assessments for pelvic organ prolapse. Curr Opin Obstet Gynecol. 15:391–394. 2003. View Article : Google Scholar : PubMed/NCBI
3	Suzme R, Yalcin O, Gurdol F, Gungor F and Bilir A: Connective tissue alterations in women with pelvic organ prolapse and urinary incontinence. Acta Obstet Gynecol Scand. 86:882–888. 2007. View Article : Google Scholar : PubMed/NCBI
4	Alperin M and Moalli PA: Remodeling of vaginal connective tissue in patients with prolapse. Curr Opin Obstet Gynecol. 18:544–550. 2006. View Article : Google Scholar : PubMed/NCBI
5	Gelse K, Pöschl E and Aigner T: Collagens - structure, function, and biosynthesis. Adv Drug Deliv Rev. 55:1531–1546. 2003. View Article : Google Scholar : PubMed/NCBI
6	Ewies AA, Al-Azzawi F and Thompson J: Changes in extracellular matrix proteins in the cardinal ligaments of post-menopausal women with or without prolapse: a computerized immunohistomorphometric analysis. Hum Reprod. 18:2189–2195. 2003. View Article : Google Scholar
7	Kerkhof MH, Hendriks L and Brölmann HA: Changes in connective tissue in patients with pelvic organ prolapse - a review of the current literature. Int Urogynecol J Pelvic Floor Dysfunct. 20:461–474. 2009. View Article : Google Scholar : PubMed/NCBI
8	Campeau L, Gorbachinsky I, Badlani GH and Andersson KE: Pelvic floor disorders: linking genetic risk factors to biochemical changes. BJU Int. 108:1240–1247. 2011. View Article : Google Scholar : PubMed/NCBI
9	Carmeliet P, Moons L, Lijnen R, et al: Urokinase-generated plasmin activates matrix metalloproteinases during aneurysm formation. Nat Genet. 17:439–444. 1997. View Article : Google Scholar : PubMed/NCBI
10	Amălinei C, Căruntu ID, Giuşcă SE and Bălan RA: Matrix metalloproteinases involvement in pathologic conditions. Rom J Morphol Embryol. 51:215–228. 2010.
11	Bourboulia D and Stetler-Stevenson WG: Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs): Positive and negative regulators in tumor cell adhesion. Semin Cancer Biol. 20:161–168. 2010. View Article : Google Scholar
12	Gabriel B, Watermann D, Hancke K, et al: Increased expression of matrix metalloproteinase 2 in uterosacral ligaments is associated with pelvic organ prolapse. Int Urogynecol J Pelvic Floor Dysfunct. 17:478–482. 2006. View Article : Google Scholar : PubMed/NCBI
13	Jackson S, James M and Abrams P: The effect of oestradiol on vaginal collagen metabolism in postmenopausal women with genuine stress incontinence. BJOG. 109:339–344. 2002. View Article : Google Scholar : PubMed/NCBI
14	Phillips CH, Anthony F, Benyon C and Monga AK: Collagen metabolism in the uterosacral ligaments and vaginal skin of women with uterine prolapse. BJOG. 113:39–46. 2006. View Article : Google Scholar : PubMed/NCBI
15	Lammers K, Lince SL, Spath MA, et al: Pelvic organ prolapse and collagen-associated disorders. Int Urogynecol J. 23:313–319. 2012. View Article : Google Scholar : PubMed/NCBI
16	Johanes I, Mihelc E, Sivasankar M and Ivanisevic A: Morphological properties of collagen fibers in porcine lamina propria. J Voice. 25:254–257. 2011. View Article : Google Scholar : PubMed/NCBI
17	Gueders MM, Foidart JM, Noel A and Cataldo DD: Matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs in the respiratory tract: potential implications in asthma and other lung diseases. Eur J Pharmacol. 533:133–144. 2006. View Article : Google Scholar
18	Chen BH, Wen Y, Li H and Polan ML: Collagen metabolism and turnover in women with stress urinary incontinence and pelvic prolapse. Int Urogynecol J Pelvic Floor Dysfunct. 13:80–87. 2002. View Article : Google Scholar : PubMed/NCBI
19	Wu JM, Visco AG, Grass EA, et al: Matrix metalloproteinase-9 genetic polymorphisms and the risk for advanced pelvic organ prolapse. Obstet Gynecol. 120:587–593. 2012. View Article : Google Scholar : PubMed/NCBI
20	Strinic T, Vulic M, Tomic S, et al: Matrix metalloproteinases-1, -2 expression in uterosacral ligaments from women with pelvic organ prolapse. Maturitas. 64:132–135. 2009. View Article : Google Scholar : PubMed/NCBI
21	Skorupski P, Jankiewicz K, Miotła P, et al: The polymorphisms of the MMP-1 and the MMP-3 genes and the risk of pelvic organ prolapse. Int Urogynecol J. 24:1033–1038. 2013. View Article : Google Scholar : PubMed/NCBI
22	Moore CS and Crocker SJ: An alternate perspective on the roles of TIMPs and MMPs in pathology. Am J Pathol. 180:12–16. 2012. View Article : Google Scholar : PubMed/NCBI