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Article

Visceral adipose tissue‑derived serine protease inhibitor inhibits interleukin‑1β‑induced catabolic and inflammatory responses in murine chondrocytes

  • Authors:
    • Jia‑Peng Bao
    • Li‑Feng Jiang
    • Jing Li
    • Wei‑Ping Chen
    • Peng‑Fei Hu
    • Li‑Dong Wu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics Surgery, The Second Hospital of Medical College, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China
  • Pages: 2191-2197
    |
    Published online on: August 11, 2014
       https://doi.org/10.3892/mmr.2014.2478
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Abstract

Visceral adipose tissue‑derived serine protease inhibitor (vaspin) is a newly identified member of the adipocytokine family, whose precise role in chondrocyte metabolism remains to be elucidated. The aim of the present study was to investigate the effect of vaspin on chondrocytes. The cell viability and the cytotoxicity of vaspin in chondrocytes were examined. Furthermore, the gene expression of matrix metalloproteinases‑2 and -9, a disintegrin and metalloproteinase with thrombospondin motifs 4 and 5 and cathepsin D was also examined, as well as the protein production of cyclooxygenase‑2, prostaglandin E2 and inducible nitrous oxide synthase following treatment with different concentrations of vaspin in the absence or presence of interleukin‑1‑beta (IL‑1β). In addition, the protein levels of the inhibitor of nuclear factor‑κB (IκB‑α) and the phosphorylation of nuclear factor kappa B (NF‑κB) were investigated. Vaspin was not able to stimulate the proliferation of chondrocytes and demonstrated no significant cytotoxic effect at concentrations of 10‑500 ng/ml following coincubation for 24 and 48 h. However, vaspin inhibited IL‑1β‑induced production of catabolic factors and inflammatory mediators in chondrocytes, and also suppressed the phosphorylation of NF‑κB and the degradation of IκB‑α. The data from the present study suggested that vaspin has a protective effect in chondrocyte metabolism and is an important factor in the pathophysiology of osteoarthritis.
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Copy and paste a formatted citation
Spandidos Publications style
Bao JP, Jiang LF, Li J, Chen WP, Hu PF and Wu LD: Visceral adipose tissue‑derived serine protease inhibitor inhibits interleukin‑1β‑induced catabolic and inflammatory responses in murine chondrocytes. Mol Med Rep 10: 2191-2197, 2014.
APA
Bao, J., Jiang, L., Li, J., Chen, W., Hu, P., & Wu, L. (2014). Visceral adipose tissue‑derived serine protease inhibitor inhibits interleukin‑1β‑induced catabolic and inflammatory responses in murine chondrocytes. Molecular Medicine Reports, 10, 2191-2197. https://doi.org/10.3892/mmr.2014.2478
MLA
Bao, J., Jiang, L., Li, J., Chen, W., Hu, P., Wu, L."Visceral adipose tissue‑derived serine protease inhibitor inhibits interleukin‑1β‑induced catabolic and inflammatory responses in murine chondrocytes". Molecular Medicine Reports 10.4 (2014): 2191-2197.
Chicago
Bao, J., Jiang, L., Li, J., Chen, W., Hu, P., Wu, L."Visceral adipose tissue‑derived serine protease inhibitor inhibits interleukin‑1β‑induced catabolic and inflammatory responses in murine chondrocytes". Molecular Medicine Reports 10, no. 4 (2014): 2191-2197. https://doi.org/10.3892/mmr.2014.2478
Copy and paste a formatted citation
x
Spandidos Publications style
Bao JP, Jiang LF, Li J, Chen WP, Hu PF and Wu LD: Visceral adipose tissue‑derived serine protease inhibitor inhibits interleukin‑1β‑induced catabolic and inflammatory responses in murine chondrocytes. Mol Med Rep 10: 2191-2197, 2014.
APA
Bao, J., Jiang, L., Li, J., Chen, W., Hu, P., & Wu, L. (2014). Visceral adipose tissue‑derived serine protease inhibitor inhibits interleukin‑1β‑induced catabolic and inflammatory responses in murine chondrocytes. Molecular Medicine Reports, 10, 2191-2197. https://doi.org/10.3892/mmr.2014.2478
MLA
Bao, J., Jiang, L., Li, J., Chen, W., Hu, P., Wu, L."Visceral adipose tissue‑derived serine protease inhibitor inhibits interleukin‑1β‑induced catabolic and inflammatory responses in murine chondrocytes". Molecular Medicine Reports 10.4 (2014): 2191-2197.
Chicago
Bao, J., Jiang, L., Li, J., Chen, W., Hu, P., Wu, L."Visceral adipose tissue‑derived serine protease inhibitor inhibits interleukin‑1β‑induced catabolic and inflammatory responses in murine chondrocytes". Molecular Medicine Reports 10, no. 4 (2014): 2191-2197. https://doi.org/10.3892/mmr.2014.2478
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