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Article

Stem cell characteristics of dormant cells and cisplatin‑induced effects on the stemness of epithelial ovarian cancer cells

  • Authors:
    • Nan Zhou
    • Xiaohua Wu
    • Bo Yang
    • Xu Yang
    • Dingding Zhang
    • Guo Qing
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China, Department of Obstetrics and Gynecology, Bethune International Peace Hospital, Shijiazhuang, Hebei 050082, P.R. China, Department of Obstetrics and Gynecology, First Hospital of Shijiazhuang, Shijiazhuang, Hebei 050011, P.R. China
  • Pages: 2495-2504
    |
    Published online on: August 12, 2014
       https://doi.org/10.3892/mmr.2014.2483
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Abstract

Tumor dormancy is a common biological property of malignancies and a leading factor in treatment failure, metastasis and tumor recurrence. The present study generated mouse xenograft models by injection of PKH26‑labeled SKOV3 ovarian cancer cells, which were divided into two groups: The control group (SKOV3‑P tumors,) and the treatment group that generated resistant tumors following prolonged administration of cisplatin (SKOV3‑R tumors). Administration of cisplatin resulted in inhibition of the tumor growth and SKOV3‑R tumors coexisted with their host at a stable size. According to fluorochrome PKH26 retention, there were multiple cell clones (PKH26hi, PKH26low and PKH26neg cells) in the single cell line generated from xenograft tumors. PKH26hi subsets in SKOV3‑P and SKOV3‑R tumors were dormant cells, as the majority were arrested in G0/G1 phase and expressed high levels of the stem cell markers Oct‑4, Nestin, CD117 and CD44. PKH26hi subsets also demonstrated greater clonogenic capability in vitro and tumorigenicity in vivo, as compared with PKH26low and PKH26neg cells. Notably, chemotherapy was demonstrated to lead to the enrichment and enhanced stem‑like characteristics of dormant/slow‑cycling PKH26hi cells. The results of the present study have demonstrated for the first time, to the best of our knowledge, that dormant tumor cells exhibit stem‑like characteristics, and that cisplatin enhances these characteristics in epithelial ovarian cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou N, Wu X, Yang B, Yang X, Zhang D and Qing G: Stem cell characteristics of dormant cells and cisplatin‑induced effects on the stemness of epithelial ovarian cancer cells. Mol Med Rep 10: 2495-2504, 2014.
APA
Zhou, N., Wu, X., Yang, B., Yang, X., Zhang, D., & Qing, G. (2014). Stem cell characteristics of dormant cells and cisplatin‑induced effects on the stemness of epithelial ovarian cancer cells. Molecular Medicine Reports, 10, 2495-2504. https://doi.org/10.3892/mmr.2014.2483
MLA
Zhou, N., Wu, X., Yang, B., Yang, X., Zhang, D., Qing, G."Stem cell characteristics of dormant cells and cisplatin‑induced effects on the stemness of epithelial ovarian cancer cells". Molecular Medicine Reports 10.5 (2014): 2495-2504.
Chicago
Zhou, N., Wu, X., Yang, B., Yang, X., Zhang, D., Qing, G."Stem cell characteristics of dormant cells and cisplatin‑induced effects on the stemness of epithelial ovarian cancer cells". Molecular Medicine Reports 10, no. 5 (2014): 2495-2504. https://doi.org/10.3892/mmr.2014.2483
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou N, Wu X, Yang B, Yang X, Zhang D and Qing G: Stem cell characteristics of dormant cells and cisplatin‑induced effects on the stemness of epithelial ovarian cancer cells. Mol Med Rep 10: 2495-2504, 2014.
APA
Zhou, N., Wu, X., Yang, B., Yang, X., Zhang, D., & Qing, G. (2014). Stem cell characteristics of dormant cells and cisplatin‑induced effects on the stemness of epithelial ovarian cancer cells. Molecular Medicine Reports, 10, 2495-2504. https://doi.org/10.3892/mmr.2014.2483
MLA
Zhou, N., Wu, X., Yang, B., Yang, X., Zhang, D., Qing, G."Stem cell characteristics of dormant cells and cisplatin‑induced effects on the stemness of epithelial ovarian cancer cells". Molecular Medicine Reports 10.5 (2014): 2495-2504.
Chicago
Zhou, N., Wu, X., Yang, B., Yang, X., Zhang, D., Qing, G."Stem cell characteristics of dormant cells and cisplatin‑induced effects on the stemness of epithelial ovarian cancer cells". Molecular Medicine Reports 10, no. 5 (2014): 2495-2504. https://doi.org/10.3892/mmr.2014.2483
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