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Desmocollin‑2 affects the adhesive strength and cytoskeletal arrangement in esophageal squamous cell carcinoma cells

  • Authors:
    • Wang‑Kai Fang
    • Lian‑Di Liao
    • Fa‑Min Zeng
    • Pi‑Xian Zhang
    • Jian‑Yi Wu
    • Jian Shen
    • Li‑Yan Xu
    • En‑Min Li
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China, Institute of Oncologic Pathology, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China, Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China
    Copyright: © Fang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2358-2364
    |
    Published online on: August 12, 2014
       https://doi.org/10.3892/mmr.2014.2485
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Abstract

Desmocollin‑2 (DSC2), a transmembrane glycoprotein belonging to the desmosomal cadherin family, has been found to be differentially expressed in several types of cancer and to be involved in tumor progression. The tumor metastasis suppressing property of DSC2 in esophageal squamous cell carcinoma (ESCC) has been described, however, its contribution to cell cohesion in ESCC remains to be elucidated. In the present study, using RNA interference (RNAi), the expression of DSC2 was silenced in SHEEC and KYSE510 cells. Hanging drop and fragmentation assays were performed to investigate the role of DSC2 in cell‑cell adhesion. Western blot analysis and confocal microscopy were used to analyze the expression and localization of cell adhesion molecules and cytoskeletal arrangement. The results demonstrated that DSC2 knock down by RNAi caused defects in cell‑cell adhesion and a concomitant reduction in desmosomal protein expression and adherens junction molecule distribution. A decrease in the expression of DSC2 caused an increase in free γ‑catenin levels, thus promoting its recruitment to the adherens junction complex. In addition, the RNAi‑mediated inhibition of DSC2 led to keratin intermediate filament retraction and filamentous‑actin cytoskeleton rearrangement. Taken together, these data support our previous findings and the proposal that DSC2 may be involved in the regulation of the invasive behavior of cells by a mechanism that controls cell‑cell attachment and cytoskeleton rearrangement.
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1 

Green KJ, Getsios S, Troyanovsky S and Godsel LM: Intercellular junction assembly, dynamics, and homeostasis. Cold Spring Harb Perspect Biol. 2:a0001252010. View Article : Google Scholar : PubMed/NCBI

2 

Angst BD, Marcozzi C and Magee AI: The cadherin superfamily: diversity in form and function. J Cell Sci. 114:629–641. 2001.PubMed/NCBI

3 

Jeanes A, Gottardi CJ and Yap AS: Cadherins and cancer: how does cadherin dysfunction promote tumor progression? Oncogene. 27:6920–6929. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Nuber UA, Schäfer S, Schmidt A, Koch PJ and Franke WW: The widespread human desmocollin Dsc2 and tissue-specific patterns of synthesis of various desmocollin subtypes. Eur J Cell Biol. 66:69–74. 1995.PubMed/NCBI

5 

Chen YJ, Chang JT, Lee L, Wang HM, Liao CT, Chiu CC, Chen PJ and Cheng AJ: DSG3 is overexpressed in head neck cancer and is a potential molecular target for inhibition of oncogenesis. Oncogene. 26:467–476. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Fang WK, Liao LD, Li LY, Xie YM, Xu XE, Zhao WJ, Wu JY, Zhu MX, Wu ZY, Du ZP, Wu BL, Xie D, Guo MZ, Xu LY and Li EM: Down-regulated desmocollin-2 promotes cell aggressiveness through redistributing adherens junctions and activating beta-catenin signalling in oesophageal squamous cell carcinoma. J Pathol. 231:257–270. 2013. View Article : Google Scholar

7 

Kolegraff K, Nava P, Helms MN, Parkos CA and Nusrat A: Loss of desmocollin-2 confers a tumorigenic phenotype to colonic epithelial cells through activation of Akt/{beta}-catenin signaling. Mol Biol Cell. 22:1121–1134. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Dusek RL, Getsios S, Chen F, Park JK, Amargo EV, Cryns VL and Green KJ: The differentiation-dependent desmosomal cadherin desmoglein 1 is a novel caspase-3 target that regulates apoptosis in keratinocytes. J Biol Chem. 281:3614–3624. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Khan K, Hardy R, Haq A, Ogunbiyi O, Morton D and Chidgey M: Desmocollin switching in colorectal cancer. Br J Cancer. 95:1367–1370. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Anami K, Oue N, Noguchi T, Sakamoto N, Sentani K, Hayashi T, Hinoi T, Okajima M, Graff JM and Yasui W: Search for transmembrane protein in gastric cancer by the Escherichia coli ampicillin secretion trap: expression of DSC2 in gastric cancer with intestinal phenotype. J Pathol. 221:275–284. 2010.PubMed/NCBI

11 

Cui T, Chen Y, Yang L, Mireskandari M, Knösel T, Zhang Q, Kohler LH, Kunze A, Presselt N and Petersen I: Diagnostic and prognostic impact of desmocollins in human lung cancer. J Clin Pathol. 65:1100–1106. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Hayashi T, Sentani K, Oue N, Anami K, Sakamoto N, Ohara S, Teishima J, Noguchi T, Nakayama H, Taniyama K, Matsubara A and Yasui W: Desmocollin 2 is a new immunohistochemical marker indicative of squamous differentiation in urothelial carcinoma. Histopathology. 59:710–721. 2011. View Article : Google Scholar

13 

Hamidov Z, Altendorf-Hofmann A, Chen Y, Settmacher U, Petersen I and Knösel T: Reduced expression of desmocollin 2 is an independent prognostic biomarker for shorter patients survival in pancreatic ductal adenocarcinoma. J Clin Pathol. 64:990–994. 2011. View Article : Google Scholar

14 

Fang WK, Gu W, Li EM, Wu ZY, Shen ZY, Shen JH, Wu JY, Pan F, Lv Z, Xu XE, Huang Q and Xu LY: Reduced membranous and ectopic cytoplasmic expression of DSC2 in esophageal squamous cell carcinoma: an independent prognostic factor. Hum Pathol. 41:1456–1465. 2010. View Article : Google Scholar

15 

Huen AC, Park JK, Godsel LM, Chen X, Bannon LJ, Amargo EV, Hudson TY, Mongiu AK, Leigh IM, Kelsell DP, Gumbiner BM and Green KJ: Intermediate filament-membrane attachments function synergistically with actin-dependent contacts to regulate intercellular adhesive strength. J Cell Biol. 159:1005–1017. 2002. View Article : Google Scholar

16 

Lewis JE, Wahl JK 3rd, Sass KM, Jensen PJ, Johnson KR and Wheelock MJ: Cross-talk between adherens junctions and desmosomes depends on plakoglobin. J Cell Biol. 136:919–934. 1997. View Article : Google Scholar : PubMed/NCBI

17 

Garrod D and Chidgey M: Desmosome structure, composition and function. Biochim Biophys Acta. 1778:572–587. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Hartlieb E, Kempf B, Partilla M, Vigh B, Spindler V and Waschke J: Desmoglein 2 is less important than desmoglein 3 for keratinocyte cohesion. PLoS One. 8:e537392013. View Article : Google Scholar : PubMed/NCBI

19 

Mannan T, Jing S, Foroushania SH, Fortune F and Wan H: RNAi-mediated inhibition of the desmosomal cadherin (desmoglein 3) impairs epithelial cell proliferation. Cell Prolif. 44:301–310. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Vasioukhin V, Bowers E, Bauer C, Degenstein L and Fuchs E: Desmoplakin is essential in epidermal sheet formation. Nat Cell Biol. 3:1076–1085. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Bass-Zubek AE, Hobbs RP, Amargo EV, Garcia NJ, Hsieh SN, Chen X, Wahl JK 3rd, Denning MF and Green KJ: Plakophilin 2: a critical scaffold for PKC alpha that regulates intercellular junction assembly. J Cell Biol. 181:605–613. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Godsel LM, Dubash AD, Bass-Zubek AE, Amargo EV, Klessner JL, Hobbs RP, Chen X and Green KJ: Plakophilin 2 couples actomyosin remodeling to desmosomal plaque assembly via RhoA. Mol Biol Cell. 21:2844–2859. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Acehan D, Petzold C, Gumper I, Sabatini DD, Müller EJ, Cowin P and Stokes DL: Plakoglobin is required for effective intermediate filament anchorage to desmosomes. J Invest Dermatol. 128:2665–2675. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Todorović V, Desai BV, Patterson MJ, Amargo EV, Dubash AD, Yin T, Jones JC and Green KJ: Plakoglobin regulates cell motility through Rho- and fibronectin-dependent Src signaling. J Cell Sci. 123:3576–3586. 2010.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Fang WK, Liao LD, Zeng FM, Zhang PX, Wu JY, Shen J, Xu LY and Li EM: Desmocollin‑2 affects the adhesive strength and cytoskeletal arrangement in esophageal squamous cell carcinoma cells. Mol Med Rep 10: 2358-2364, 2014.
APA
Fang, W., Liao, L., Zeng, F., Zhang, P., Wu, J., Shen, J. ... Li, E. (2014). Desmocollin‑2 affects the adhesive strength and cytoskeletal arrangement in esophageal squamous cell carcinoma cells. Molecular Medicine Reports, 10, 2358-2364. https://doi.org/10.3892/mmr.2014.2485
MLA
Fang, W., Liao, L., Zeng, F., Zhang, P., Wu, J., Shen, J., Xu, L., Li, E."Desmocollin‑2 affects the adhesive strength and cytoskeletal arrangement in esophageal squamous cell carcinoma cells". Molecular Medicine Reports 10.5 (2014): 2358-2364.
Chicago
Fang, W., Liao, L., Zeng, F., Zhang, P., Wu, J., Shen, J., Xu, L., Li, E."Desmocollin‑2 affects the adhesive strength and cytoskeletal arrangement in esophageal squamous cell carcinoma cells". Molecular Medicine Reports 10, no. 5 (2014): 2358-2364. https://doi.org/10.3892/mmr.2014.2485
Copy and paste a formatted citation
x
Spandidos Publications style
Fang WK, Liao LD, Zeng FM, Zhang PX, Wu JY, Shen J, Xu LY and Li EM: Desmocollin‑2 affects the adhesive strength and cytoskeletal arrangement in esophageal squamous cell carcinoma cells. Mol Med Rep 10: 2358-2364, 2014.
APA
Fang, W., Liao, L., Zeng, F., Zhang, P., Wu, J., Shen, J. ... Li, E. (2014). Desmocollin‑2 affects the adhesive strength and cytoskeletal arrangement in esophageal squamous cell carcinoma cells. Molecular Medicine Reports, 10, 2358-2364. https://doi.org/10.3892/mmr.2014.2485
MLA
Fang, W., Liao, L., Zeng, F., Zhang, P., Wu, J., Shen, J., Xu, L., Li, E."Desmocollin‑2 affects the adhesive strength and cytoskeletal arrangement in esophageal squamous cell carcinoma cells". Molecular Medicine Reports 10.5 (2014): 2358-2364.
Chicago
Fang, W., Liao, L., Zeng, F., Zhang, P., Wu, J., Shen, J., Xu, L., Li, E."Desmocollin‑2 affects the adhesive strength and cytoskeletal arrangement in esophageal squamous cell carcinoma cells". Molecular Medicine Reports 10, no. 5 (2014): 2358-2364. https://doi.org/10.3892/mmr.2014.2485
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