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Effects of lentiviral infection of mesenchymal stem cells on the expression of octamer transcription factor 4

  • Authors:
    • Jing Chang
    • Li Tang
    • Han Lei
    • Xiao-Gang Zhang
    • Zhong Zuo
    • Wei Huang
    • Hang Fu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Chang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2249-2254
    |
    Published online on: August 21, 2014
       https://doi.org/10.3892/mmr.2014.2505
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Abstract

The present study aimed to investigate the effects of lentiviral infection of human umbilical cord mesenchymal stem cells (hUCMSCs) on the expression of octamer transcription factor 4 (Oct4). hUCMSCs were infected with lentivirus carrying the green fluorescent protein gene (GFP) at different multiplicities of infection (MOI), and the optimal MOI was determined by flow cytometry; the proliferation of non‑infected and GFP-carrying lentivirus‑infected hUCMSCs was evaluated by the MTT assay; and the expression of the Oct4 gene was measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunofluorescence staining in hUCMSCs cultured in vitro for eight weeks. Positive GFP staining of hUCMSCs was estimated at >75% at 48 h following infection with the GFP-carrying lentivirus (MOI = 20); no effect on hUCMSC proliferation was detected by the MTT assay following the infection; immunofluorescence analysis detected positive Oct4 expression in the cell nuclei at two and eight weeks of culture, while the relative expression of Oct4 assessed by qRT-PCR was 0.9075±0.0124. The GFP gene carried by the lentivirus was successfully expressed in hUCMSCs and had no significant effect on Oct4 expression, which lays a solid foundation for future studies investigating gene functions via the use of exogenous markers.
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Copy and paste a formatted citation
Spandidos Publications style
Chang J, Tang L, Lei H, Zhang X, Zuo Z, Huang W and Fu H: Effects of lentiviral infection of mesenchymal stem cells on the expression of octamer transcription factor 4. Mol Med Rep 10: 2249-2254, 2014.
APA
Chang, J., Tang, L., Lei, H., Zhang, X., Zuo, Z., Huang, W., & Fu, H. (2014). Effects of lentiviral infection of mesenchymal stem cells on the expression of octamer transcription factor 4. Molecular Medicine Reports, 10, 2249-2254. https://doi.org/10.3892/mmr.2014.2505
MLA
Chang, J., Tang, L., Lei, H., Zhang, X., Zuo, Z., Huang, W., Fu, H."Effects of lentiviral infection of mesenchymal stem cells on the expression of octamer transcription factor 4". Molecular Medicine Reports 10.5 (2014): 2249-2254.
Chicago
Chang, J., Tang, L., Lei, H., Zhang, X., Zuo, Z., Huang, W., Fu, H."Effects of lentiviral infection of mesenchymal stem cells on the expression of octamer transcription factor 4". Molecular Medicine Reports 10, no. 5 (2014): 2249-2254. https://doi.org/10.3892/mmr.2014.2505
Copy and paste a formatted citation
x
Spandidos Publications style
Chang J, Tang L, Lei H, Zhang X, Zuo Z, Huang W and Fu H: Effects of lentiviral infection of mesenchymal stem cells on the expression of octamer transcription factor 4. Mol Med Rep 10: 2249-2254, 2014.
APA
Chang, J., Tang, L., Lei, H., Zhang, X., Zuo, Z., Huang, W., & Fu, H. (2014). Effects of lentiviral infection of mesenchymal stem cells on the expression of octamer transcription factor 4. Molecular Medicine Reports, 10, 2249-2254. https://doi.org/10.3892/mmr.2014.2505
MLA
Chang, J., Tang, L., Lei, H., Zhang, X., Zuo, Z., Huang, W., Fu, H."Effects of lentiviral infection of mesenchymal stem cells on the expression of octamer transcription factor 4". Molecular Medicine Reports 10.5 (2014): 2249-2254.
Chicago
Chang, J., Tang, L., Lei, H., Zhang, X., Zuo, Z., Huang, W., Fu, H."Effects of lentiviral infection of mesenchymal stem cells on the expression of octamer transcription factor 4". Molecular Medicine Reports 10, no. 5 (2014): 2249-2254. https://doi.org/10.3892/mmr.2014.2505
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