Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
November-2014 Volume 10 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2014 Volume 10 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Duhuo Jisheng Decoction‑containing serum promotes proliferation of interleukin‑1β‑induced chondrocytes through the p16‑cyclin D1/CDK4‑Rb pathway

  • Authors:
    • Guangwen Wu
    • Huailing Fan
    • Yuanpeng Huang
    • Chunsong Zheng
    • Jinxia Ye
    • Xianxiang Liu
  • View Affiliations / Copyright

    Affiliations: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fujian, Fuzhou 350122, P.R. China, Academy of Traditional Chinese Medicine, Fujian University of Traditional Chinese Medicine, Fujian, Fuzhou 350122, P.R. China, Fujian Sports Vocational Education and Technical College, Fujian, Fuzhou 350003, P.R. China
  • Pages: 2525-2534
    |
    Published online on: September 2, 2014
       https://doi.org/10.3892/mmr.2014.2527
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Duhuo Jisheng Decoction (DHJSD) is a traditional Chinese herbal medicine that has multiple uses, including as a treatment for osteoarthritis (OA). However, the molecular mechanisms underlying the therapeutic effects of DHJSD on OA remain unknown. In the present study, a serum pharmacological method was applied to investigate the effects of DHJSD on the proliferation of chondrocytes treated with interleukin‑1β (IL‑1β) in vitro. This is a cell model commonly used to reproduce the mechanisms involved in degenerative arthropathies, including OA. The most effective intervention conditions of DHJSD serum were examined by MTT assay. The degenerative chondrocyte model was established by IL‑1β‑culture for 24 h, and was verified by optical microscopy and immunohistochemical analyses. Following the successful establishment of the degenerative chondrocyte model, the chondrocytes were subsequently randomly divided into two groups: The blank serum group and the DHJSD treatment group. Subsequent to treatment with the corresponding serum, cell proliferation was detected by MTT assay and DNA staining followed by FACS analysis, and the mRNA and protein expression levels of cyclin D1, cyclin‑dependent kinase 4 (CDK4), retinoblastoma tumor suppressor protein (Rb) and p16 were measured by reverse transcription polymerase chain reaction and western blotting, respectively. The results indicated that the most effective condition for the promotion of chondrocyte proliferation was 10% concentration of DHJSD 2‑h serum, and the degenerative chondrocyte model was successfully reproduced by IL‑1β‑treatment for 24 h. The mRNA and protein expression levels of cyclin D1, CDK4 and Rb in the DHJSD serum‑treated cells were significantly increased compared with those in the blank serum group, whereas p16 expression was significantly downregulated. These results indicate that treatment of cells with DHJSD‑containing serum is able to promote IL‑1β‑induced chondrocyte proliferation by promoting G1/S phase transition via modulating the expressions of cyclin D1, CDK4, Rb and p16, which contribute to the clinical efficacy of DHJSD in OA.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Felson DT: Clinical practice. Osteoarthritis of the knee. N Engl J Med. 354:841–848. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Zhang W, Nuki G, Moskowitz RW, et al: OARSI recommendations for the management of hip and knee osteoarthritis: part III: Changes in evidence following systematic cumulative update of research published through January 2009. Osteoarthritis Cartilage. 18:476–499. 2010. View Article : Google Scholar

3 

Qiu G: Osteoarthritis diagnosis and treatment guidelines. Chinese J Joint Surgery. 1:281–285. 2007.

4 

Shortkroff S and Yates KE: Alteration of matrix glycosaminoglycans diminishes articular chondrocytes’ response to a canonical Wnt signal. Osteoarthritis Cartilage. 15:147–154. 2007.PubMed/NCBI

5 

Chan BY, Fuller ES, Russell AK, et al: Increased chondrocyte sclerostin may protect against cartilage degradation in osteoarthritis. Osteoarthritis Cartilage. 19:874–885. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Huang JG, Xia C, Zheng XP, et al: 17β-Estradiol promotes cell proliferation in rat osteoarthritis model chondrocytes via PI3K/Akt pathway. Cell Mol Biol Lett. 16:564–575. 2011.

7 

Kashiwagi A, Schipani E, Fein MJ, Greer PA and Shimada M: Targeted deletion of Capn4 in cells of the chondrocyte lineage impairs chondrocyte proliferation and differentiation. Mol Cell Biol. 30:2799–2810. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Sarzi-Puttini P, Cimmino MA, Scarpa R, et al: Osteoarthritis: an overview of the disease and its treatment strategies. Semin Arthritis Rheum. 35(Suppl 1): 1–10. 2005. View Article : Google Scholar

9 

Badley EM, Rasooly I and Webster GK: Relative importance of musculoskeletal disorders as a cause of chronic health problems, disability, and health care utilization: findings from the 1990 Ontario Health Survey. J Rheumatol. 21:505–514. 1994.

10 

Felson DT, Lawrence RC, Dieppe PA, et al: Osteoarthritis: new insights. Part 1: the disease and its risk factors. Ann Intern Med. 133:635–646. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Bradley JD, Brandt KD, Katz BP, Kalasinski LA and Ryan SI: Comparison of an antiinflammatory dose of ibuprofen, an analgesic dose of ibuprofen, and acetaminophen in the treatment of patients with osteoarthritis of the knee. N Engl J Med. 325:87–91. 1991. View Article : Google Scholar : PubMed/NCBI

12 

Lohmander LS, Dalén N, Englund G, et al: Intra-articular hyaluronan injections in the treatment of osteoarthritis of the knee: a randomised, double blind, placebo controlled multicentre trial. Ann Rheum Dis. 55:424–431. 1996. View Article : Google Scholar : PubMed/NCBI

13 

Berenbaum F: New horizons and perspectives in the treatment of osteoarthritis. Arthritis Res Ther. 10(Suppl 2): S12008. View Article : Google Scholar : PubMed/NCBI

14 

Ahmed S, Anuntiyo J, Malemud CJ and Haqqi TM: Biological basis for the use of botanicals in osteoarthritis and rheumatoid arthritis: a review. Evid Based Complement Alternat Med. 2:301–308. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Parkman CA: Alternative therapies for osteoarthritis. Case Manager. 12:34–36. 2001. View Article : Google Scholar

16 

Teekachunhatean S, Kunanusorn P, Rojanasthien N, et al: Chinese herbal recipe versus diclofenac in symptomatic treatment of osteoarthritis of the knee: a randomized controlled trial [ISRCTN70292892]. BMC Complement Altern Med. 4:192004.PubMed/NCBI

17 

Setty AR and Sigal LH: Herbal medications commonly used in the practice of rheumatology: mechanisms of action, efficacy, and side effects. Semin Arthritis Rheum. 34:773–784. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Chun SC, Jee SY, Lee SG, Park SJ, Lee JR and Kim SC: Anti-inflammatory activity of the methanol extract of moutan cortex in LPS-activated Raw264.7 cells. Evid Based Complement Alternat Med. 4:327–333. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Wu M and Gu Z: Screening of bioactive compounds from moutan cortex and their anti-inflammatory activities in rat synoviocytes. Evid Based Complement Alternat Med. 6:57–63. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Sun SM: Bei Ji Qian Jin Yao Fang. 8. 1st edition. People’s Medical Publishing Press; Beijing: pp. 166–167. 1982

21 

Lai JN, Chen HJ, Chen CC, Lin JH, Hwang JS and Wang JD: Duhuo jisheng tang for treating osteoarthritis of the knee: a prospective clinical observation. Chin Med. 2:42007. View Article : Google Scholar : PubMed/NCBI

22 

Zheng CS, XXJ, Ye HZ, Wu GW, Li XH, Huang SP and Liu XX: Computational approaches for exploring the potential synergy and polypharmacology of Duhuo Jisheng Decoction in the therapy of osteoarthritis. Mol Med Report. 7:1812–1818. 2013.PubMed/NCBI

23 

Wu G, Chen W, Fan H, et al: Duhuo Jisheng Decoction promotes chondrocyte proliferation through accelerated G1/S transition in osteoarthritis. Int J Mol Med. 32:1001–1010. 2013.PubMed/NCBI

24 

Panico AM, Cardile V, Garufi F, Puglia C, Bonina F and Ronsisvalle G: Effect of hyaluronic acid and polysaccharides from Opuntia ficus indica (L.) cladodes on the metabolism of human chondrocyte cultures. J Ethnopharmacol. 111:315–321. 2007.PubMed/NCBI

25 

The Ministry of Science and Technology of the People’s Republic of China. Guidance Suggestions for the Care and Use of Laboratory Animals. 2006.

26 

Huang J, Huang X, Chen Z, Zheng Q and Sun R: Dose conversion among different animals and healthy volunteers in pharmacological study. Chin J Clin Pharmacol Ther. 9:1069–1072. 2004.(In Chinese).

27 

Li XH, Du M, Liu XX, et al: Millimeter wave treatment inhibits NO-induced apoptosis of chondrocytes through the p38MAPK pathway. Int J Mol Med. 25:393–399. 2010.PubMed/NCBI

28 

Li XH, Wu MX, YHZ, Chen WL, Lin JM, Zheng LP and Liu XX: Experimental study on the suppression of sodium nitroprussiate-induced chondrocyte apoptosis by tougu xiaotong capsule-containing serum. Chin J Integr Med. 17:436–443. 2011.(In Chinese).

29 

Sanchez C, Mathy-Hartert M, Deberg MA, Ficheux H, Reginster JY and Henrotin YE: Effects of rhein on human articular chondrocytes in alginate beads. Biochem Pharmacol. 65:377–388. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Wu SQ, Otero M, Unger FM, et al: Anti-inflammatory activity of an ethanolic Caesalpinia sappan extract in human chondrocytes and macrophages. J Ethnopharmacol. 138:364–372. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Mankin HJ: The response for articular cartilage to mechanical injury. J Bone Joint Surg Am. 64:460–466. 1982.PubMed/NCBI

32 

Brandt KD and Mazzuca SA: Lessons learned from nine clinical trials of disease-modifying osteoarthritis drugs. Arthritis Rheum. 52:3349–3359. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Bensky D, Gamble A and Stöger E: Chinese Herbal Medicine: Materia Medica. 3rd edition. Eastland Press; Seattle, WA: 2004

34 

Ho LJ and Lai JH: Chinese herbs as immunomodulators and potential disease-modifying antirheumatic drugs in autoimmune disorders. Curr Drug Metab. 5:181–192. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Tashino S: ‘Serum pharmacology’ and ‘serum pharmaceutical chemistry’: from pharmacology of Chinese traditional medicines to start a new measurement of drug concentration in blood. Ther Drug Monit Res. 5:54–64. 1988.

36 

Liu N, Liu JT, Ji YY and Lu PP: Dahuang zhechong pill containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-extracellular regulated kinase 1/2 signaling. Chin J Integr Med. 18:371–377. 2012.(In Chinese).

37 

Dang XY, Dong L, Shi HT and Zou BC: Effects of serum containing Chinese medicine Sanpi Pingwei formula on proliferation and apoptosis of human SGC-7901 cells. Chin J Integr Med. 19:119–126. 2013. View Article : Google Scholar : PubMed/NCBI

38 

Wang WJ, Li DJ, Li J and Zhou WJ: An in vitro study on neuroprotective effects of serum containing Gengnianchun decoction and its main monomers against amyloid beta protein-induced cellular toxicity. J Chin Integr Med. 8:67–73. 2010.(In Chinese).

39 

Henrotin Y, Sanchez C and Reginster JY: The inhibition of metalloproteinases to treat osteoarthritis: reality and new perspectives. Expert Opin Ther Patents. 12:29–43. 2002. View Article : Google Scholar

40 

Tetlow LC, Adlam DJ and Woolley DE: Matrix metalloproteinase and proinflammatory cytokine production by chondrocytes of human osteoarthritic cartilage. Arthritis Rheum. 44:585–594. 2001. View Article : Google Scholar : PubMed/NCBI

41 

Martel-Pelletier J, Di Battista J and Lajeunesse D: Biochemical factors in joint articular tissue degradation in osteoarthritis. Osteoarthritis: Clinical and Experimental Aspects. Reginster J-YL, Pelletier JP, Martel-Pelletier J and Henrotin YE: Springer; Berlin: pp. 156–187. 1999, View Article : Google Scholar

42 

Park K, Hoffmeister B, Han DK and Hasty K: Therapeutic ultrasound effects on interleukin-1beta stimulated cartilage construct in vitro. Ultrasound Med Biol. 33:286–295. 2007. View Article : Google Scholar : PubMed/NCBI

43 

Tan L, Peng H, Osaki M, Choy BK, Auron PE, Sandell LJ and Goldring MB: Egr-1 mediates transcriptional repression of COL2A1 promoter activity by interleukin-1beta. J Biol Chem. 278:17688–17700. 2003. View Article : Google Scholar : PubMed/NCBI

44 

Gibson SL, Dai CY, Lee HW, et al: Inhibition of colon tumor progression and angiogenesis by the Ink4a/Arf locus. Cancer Res. 63:742–746. 2003.PubMed/NCBI

45 

Massagué J: G1 cell-cycle control and cancer. Nature. 432:298–306. 2004.

46 

Musgrove EA, Caldon CE, Barraclough J, Stone A and Sutherland RL: Cyclin D as a therapeutic target in cancer. Nat Rev Cancer. 11:558–572. 2011. View Article : Google Scholar : PubMed/NCBI

47 

Li J, Poi MJ and Tsai MD: Regulatory mechanisms of tumor suppressor P16(INK4A) and their relevance to cancer. Biochemistry. 50:5566–5582. 2011. View Article : Google Scholar : PubMed/NCBI

48 

Witkiewicz AK, Knudsen KE, Dicker AP and Knudsen ES: The meaning of p16(ink4a) expression in tumors: functional significance, clinical associations and future developments. Cell Cycle. 10:2497–2503. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wu G, Fan H, Huang Y, Zheng C, Ye J and Liu X: Duhuo Jisheng Decoction‑containing serum promotes proliferation of interleukin‑1β‑induced chondrocytes through the p16‑cyclin D1/CDK4‑Rb pathway. Mol Med Rep 10: 2525-2534, 2014.
APA
Wu, G., Fan, H., Huang, Y., Zheng, C., Ye, J., & Liu, X. (2014). Duhuo Jisheng Decoction‑containing serum promotes proliferation of interleukin‑1β‑induced chondrocytes through the p16‑cyclin D1/CDK4‑Rb pathway. Molecular Medicine Reports, 10, 2525-2534. https://doi.org/10.3892/mmr.2014.2527
MLA
Wu, G., Fan, H., Huang, Y., Zheng, C., Ye, J., Liu, X."Duhuo Jisheng Decoction‑containing serum promotes proliferation of interleukin‑1β‑induced chondrocytes through the p16‑cyclin D1/CDK4‑Rb pathway". Molecular Medicine Reports 10.5 (2014): 2525-2534.
Chicago
Wu, G., Fan, H., Huang, Y., Zheng, C., Ye, J., Liu, X."Duhuo Jisheng Decoction‑containing serum promotes proliferation of interleukin‑1β‑induced chondrocytes through the p16‑cyclin D1/CDK4‑Rb pathway". Molecular Medicine Reports 10, no. 5 (2014): 2525-2534. https://doi.org/10.3892/mmr.2014.2527
Copy and paste a formatted citation
x
Spandidos Publications style
Wu G, Fan H, Huang Y, Zheng C, Ye J and Liu X: Duhuo Jisheng Decoction‑containing serum promotes proliferation of interleukin‑1β‑induced chondrocytes through the p16‑cyclin D1/CDK4‑Rb pathway. Mol Med Rep 10: 2525-2534, 2014.
APA
Wu, G., Fan, H., Huang, Y., Zheng, C., Ye, J., & Liu, X. (2014). Duhuo Jisheng Decoction‑containing serum promotes proliferation of interleukin‑1β‑induced chondrocytes through the p16‑cyclin D1/CDK4‑Rb pathway. Molecular Medicine Reports, 10, 2525-2534. https://doi.org/10.3892/mmr.2014.2527
MLA
Wu, G., Fan, H., Huang, Y., Zheng, C., Ye, J., Liu, X."Duhuo Jisheng Decoction‑containing serum promotes proliferation of interleukin‑1β‑induced chondrocytes through the p16‑cyclin D1/CDK4‑Rb pathway". Molecular Medicine Reports 10.5 (2014): 2525-2534.
Chicago
Wu, G., Fan, H., Huang, Y., Zheng, C., Ye, J., Liu, X."Duhuo Jisheng Decoction‑containing serum promotes proliferation of interleukin‑1β‑induced chondrocytes through the p16‑cyclin D1/CDK4‑Rb pathway". Molecular Medicine Reports 10, no. 5 (2014): 2525-2534. https://doi.org/10.3892/mmr.2014.2527
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team