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Opposite effects of cefoperazone and ceftazidime on S‑ribosylhomocysteine lyase/autoinducer-2 quorum sensing and biofilm formation by an Escherichia coli clinical isolate

  • Authors:
    • Hui-Qing Shi
    • Feng-Jun Sun
    • Jian-Hong Chen
    • Xiao-Lan Yong
    • Qian-Yi Ou
    • Wei Feng
    • Pei-Yuan Xia
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China, Department of Clinical Pharmacy, General Hospital of Chengdu Military Region, Chengdu, Sichuan 610083, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2334-2340
    |
    Published online on: September 5, 2014
       https://doi.org/10.3892/mmr.2014.2540
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Abstract

To investigate the effects of subminimum inhibitory concentrations of cephalosporins on bacterial biofilm formation, the biofilm production of 52 Escherichia (E.) coli strains was examined following treatment with cephalosporin compounds at 1/4 minimum inhibitory concentrations (MICs). Ceftazidime (CAZ) inhibited biofilm formation in seven isolates, while cefoperazone (CFP) enhanced biofilm formation in 18 isolates. Biofilm formation of E. coli E42 was inhibited by CAZ and induced by CFP. Therefore, using reverse transcription‑polymerase chain reaction, the expression of the biofilm‑modulating genes of this isolate was investigated. To monitor the production of the autoinducer of quorum sensing in E. coli, autoinducer‑2 (AI‑2) production was detected by measuring the bioluminescence response of Vibrio harveyi BB170. Antisense oligonucleotides (AS‑ODNs) targeting S‑ribosylhomocysteine lyase (luxS) inhibited the expression of the luxS gene in E. coli. CAZ at 1/4 MIC reduced luxS mRNA levels and the production of AI‑2, whereas CFP at 1/4 MIC had the opposite effect. AS‑ODNs targeting luxS significantly decreased the aforementioned inhibitory effects of CAZ and the induction effects of CFP on E. coli biofilm formation. Therefore, biofilm formation by the E. coli clinical isolate E42 was evoked by CFP but attenuated by CAZ at sub‑MICs, via a luxS/AI‑2‑based quorum sensing system.
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Copy and paste a formatted citation
Spandidos Publications style
Shi H, Sun F, Chen J, Yong X, Ou Q, Feng W and Xia P: Opposite effects of cefoperazone and ceftazidime on S‑ribosylhomocysteine lyase/autoinducer-2 quorum sensing and biofilm formation by an Escherichia coli clinical isolate. Mol Med Rep 10: 2334-2340, 2014.
APA
Shi, H., Sun, F., Chen, J., Yong, X., Ou, Q., Feng, W., & Xia, P. (2014). Opposite effects of cefoperazone and ceftazidime on S‑ribosylhomocysteine lyase/autoinducer-2 quorum sensing and biofilm formation by an Escherichia coli clinical isolate. Molecular Medicine Reports, 10, 2334-2340. https://doi.org/10.3892/mmr.2014.2540
MLA
Shi, H., Sun, F., Chen, J., Yong, X., Ou, Q., Feng, W., Xia, P."Opposite effects of cefoperazone and ceftazidime on S‑ribosylhomocysteine lyase/autoinducer-2 quorum sensing and biofilm formation by an Escherichia coli clinical isolate". Molecular Medicine Reports 10.5 (2014): 2334-2340.
Chicago
Shi, H., Sun, F., Chen, J., Yong, X., Ou, Q., Feng, W., Xia, P."Opposite effects of cefoperazone and ceftazidime on S‑ribosylhomocysteine lyase/autoinducer-2 quorum sensing and biofilm formation by an Escherichia coli clinical isolate". Molecular Medicine Reports 10, no. 5 (2014): 2334-2340. https://doi.org/10.3892/mmr.2014.2540
Copy and paste a formatted citation
x
Spandidos Publications style
Shi H, Sun F, Chen J, Yong X, Ou Q, Feng W and Xia P: Opposite effects of cefoperazone and ceftazidime on S‑ribosylhomocysteine lyase/autoinducer-2 quorum sensing and biofilm formation by an Escherichia coli clinical isolate. Mol Med Rep 10: 2334-2340, 2014.
APA
Shi, H., Sun, F., Chen, J., Yong, X., Ou, Q., Feng, W., & Xia, P. (2014). Opposite effects of cefoperazone and ceftazidime on S‑ribosylhomocysteine lyase/autoinducer-2 quorum sensing and biofilm formation by an Escherichia coli clinical isolate. Molecular Medicine Reports, 10, 2334-2340. https://doi.org/10.3892/mmr.2014.2540
MLA
Shi, H., Sun, F., Chen, J., Yong, X., Ou, Q., Feng, W., Xia, P."Opposite effects of cefoperazone and ceftazidime on S‑ribosylhomocysteine lyase/autoinducer-2 quorum sensing and biofilm formation by an Escherichia coli clinical isolate". Molecular Medicine Reports 10.5 (2014): 2334-2340.
Chicago
Shi, H., Sun, F., Chen, J., Yong, X., Ou, Q., Feng, W., Xia, P."Opposite effects of cefoperazone and ceftazidime on S‑ribosylhomocysteine lyase/autoinducer-2 quorum sensing and biofilm formation by an Escherichia coli clinical isolate". Molecular Medicine Reports 10, no. 5 (2014): 2334-2340. https://doi.org/10.3892/mmr.2014.2540
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