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Article

Tanshinol protects human umbilical vein endothelial cells against hydrogen peroxide‑induced apoptosis

  • Authors:
    • Wei Song
    • Jun Pu
    • Ben He
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, P.R. China
  • Pages: 2764-2770
    |
    Published online on: September 5, 2014
       https://doi.org/10.3892/mmr.2014.2541
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Abstract

The present study was designed to investigate the effect of tanshinol on hydrogen peroxide (H2O2)‑induced apoptosis in human umbilical vein endothelial cells (HUVECs), and to determine the underlying mechanisms. HUVECs were pre‑incubated with tanshinol (25‑200 µM) for 24 h, followed by an incubation with 600 µM H2O2 for 6 h. The cell viability was assessed using MTT reagent and the level of cell death was determined by measuring lactate dehydrogenase (LDH) activity. Superoxide dismutase (SOD) activity, levels of malondialdehyde (MDA), reactive oxygen species (ROS) production and NADPH oxidase activity were measured spectrophotometrically using commercially available kits. The apoptotic rate of the HUVECs was detected using Annexin‑V/propidium iodide (PI) staining, followed by flow cytometry analysis using a fluorescence microscope. The protein expression of SOD‑1, SOD‑2, B‑cell lymphoma‑2 (Bcl‑2), cytochrome c and caspase‑3 was determined by western blot analysis. Pretreatment with tanshinol resulted in a significant increase in the cellular viability of HUVECs and SOD activity, and a decrease of cell apoptosis, MDA levels and ROS production, induced by H2O2. These findings were accompanied by the upregulation of Bcl‑2 protein expression, reduction in the release of cytochrome c from the mitochondria to the cytosol and a downregulation of caspase‑3 protein expression. This study showed that tanshinol protects against atherosclerosis by preventing H2O2‑induced apoptosis of HUVECs. These effects appear to be mediated by enhancing the antioxidant defenses and preserving the mitochondrial function of the cells.
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1 

Bai X, Wang X and Xu Q: Endothelial damage and stem cell repair in atherosclerosis. Vasc Pharmacol. 52:224–229. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Birukov KG: Cyclic stretch, reactive oxygen species, and vascular remodeling. Antioxid Redox Signal. 11:1651–1667. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Urso C and Caimi G: Oxidative stress and endothelial dysfunction. Minerva Med. 102:59–77. 2011.(In Italian).

4 

Irani K: Oxidant signaling in vascular cell growth, death, and survival: a review of the roles of reactive oxygen species in smooth muscle and endothelial cell mitogenic and apoptotic signaling. Circ Res. 87:179–183. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Boueiz A and Hassoun PM: Regulation of endothelial barrier function by reactive oxygen and nitrogen species. Microvasc Res. 77:26–34. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Nakamura H, Nakamura K and Yodoi J: Redox regulation of cellular activation. Annu Rev Immunol. 15:351–369. 1997. View Article : Google Scholar : PubMed/NCBI

7 

Yang B, Oo TN and Rizzo V: Lipid rafts mediate H2O2 prosurvival effects in cultured endothelial cells. FASEB J. 20:1501–1503. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Hao CN, Geng YJ, Li F, Yang T, Su DF, Duan JL and Li Y: Insulin-like growth factor-1 receptor activation prevents hydrogen peroxide-induced oxidative stress, mitochondrial dysfunction and apoptosis. Apoptosis. 16:1118–1127. 2011. View Article : Google Scholar

9 

Jia Y, Ji L, Zhang S, Xu L, Yin L, Li L, et al: Total flavonoids from Rosa Laevigata Michx fruit attenuates hydrogen peroxide induced injury in human umbilical vein endothelial cells. Food Chem Toxicol. 50:3133–3141. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Yang RX, Huang SY, Yan FF, Lu XT, Xing YF, Liu Y, et al: Danshensu protects vascular endothelia in a rat model of hyperhomocysteinemia. Acta Pharmacol Sin. 31:1395–1400. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Cheng TO: Cardiovascular effects of Danshen. Int J Cardiol. 121:9–22. 2007. View Article : Google Scholar

12 

Wang F, Liu YY, Liu LY, Zeng QJ, Wang CS, Sun K, et al: The attenuation effect of 3,4-dihydroxy-phenyl lactic acid and salvianolic acid B on venular thrombosis induced in rat mesentery by photochemical reaction. Clin Hemorheol Microcirc. 42:7–18. 2009.

13 

Han JY, Horie Y, Fan JY, Sun K, Guo J, Miura S and Hibi T: Potential of 3,4-dihydroxy-phenyl lactic acid for ameliorating ischemia-reperfusion-induced microvascular disturbance in rat mesentery. Am J Physiol Gastrointest Liver Physiol. 296:G36–G44. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Chan K, Chui SH, Wong DY, Ha WY, Chan CL and Wong RN: Protective effects of Danshensu from the aqueous extract of Salvia miltiorrhiza (Danshen) against homocysteine-induced endothelial dysfunction. Life Sci. 75:3157–3171. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Yang GD, Zhang H, Lin R, Wang WR, Shi XL, Liu Y and Ji QL: Down-regulation of CD40 gene expression and inhibition of apoptosis with Danshensu in endothelial cells. Basic Clin Pharmacol Toxicol. 104:87–92. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Jaffe EA, Nachman RL, Becker CG and Minick CR: Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 52:2745–2756. 1973. View Article : Google Scholar : PubMed/NCBI

17 

Liu L, Gu L, Ma Q, Zhu D and Huang X: Resveratrol attenuates hydrogen peroxide-induced apoptosis in human umbilical vein endothelial cells. Eur Rev Med Pharmacol Sci. 17:88–94. 2013.PubMed/NCBI

18 

Wen YD, Wang H, Kho SH, Rinkiko S, Sheng X, Shen HM and Zhu YZ: Hydrogen sulfide protects HUVECs against hydrogen peroxide induced mitochondrial dysfunction and oxidative stress. PLoS One. 8:e531472013. View Article : Google Scholar

19 

Qian J, Jiang F, Wang B, Yu Y, Zhang X, Yin Z and Liu C: Ophiopogonin D prevents H2O2-induced injury in primary human umbilical vein endothelial cells. J Ethnopharmacol. 128:438–445. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Wang YK, Hong YJ, Wei M, Wu Y, Huang ZQ, Chen RZ and Chen HZ: Curculigoside attenuates human umbilical vein endothelial cell injury induced by H2O2. J Ethnopharmacol. 132:233–239. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Fukai T and Ushio-Fukai M: Superoxide dismutases: role in redox signaling, vascular function, and diseases. Antioxid Redox Signal. 15:1583–1606. 2011. View Article : Google Scholar

22 

Hu L, Sun Y and Hu J: Catalpol inhibits apoptosis in hydrogen peroxide-induced endothelium by activating the PI3K/Akt signaling pathway and modulating expression of Bcl-2 and Bax. Eur J Pharmacol. 628:155–163. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Li ZL, Liu JC, Hu J, Li XQ, Wang SW, Yi DH and Zhao MG: Protective effects of hyperoside against human umbilical vein endothelial cell damage induced by hydrogen peroxide. J Ethnopharmacol. 139:388–394. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Duan W, Yang Y, Yi W, Yan J, Liang Z, Wang N, et al: New role of JAK2/STAT3 signaling in endothelial cell oxidative stress injury and protective effect of melatonin. PLoS One. 8:e579412013. View Article : Google Scholar : PubMed/NCBI

25 

Kane DJ, Sarafian TA, Anton R, Hahn H, Gralla EB, Valentine JS, et al: Bcl-2 inhibition of neural death: decreased generation of reactive oxygen species. Science. 262:1274–1277. 1993. View Article : Google Scholar : PubMed/NCBI

26 

Hockenbery DM, Oltvai ZN, Yin XM, Milliman CL and Korsmeyer SJ: Bcl-2 functions in an antioxidant pathway to prevent apoptosis. Cell. 75:241–251. 1993. View Article : Google Scholar : PubMed/NCBI

27 

Shroff EH, Snyder C and Chandel NS: Role of Bcl-2 family members in anoxia induced cell death. Cell Cycle. 6:807–809. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Wong WW and Puthalakath H: Bcl-2 family proteins: the sentinels of the mitochondrial apoptosis pathway. IUBMB Life. 60:390–397. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Lalier L, Cartron PF, Juin P, Nedelkina S, Manon S, Bechinger B and Vallette FM: Bax activation and mitochondrial insertion during apoptosis. Apoptosis. 12:887–896. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Chen WL, Qian Y, Meng WF, Pang JY, Lin YC, Guan YY, Chen SP, Liu J, Pei Z and Wang GL: A novel marine compound xyloketal B protects against oxidized LDL-induced cell injury in vitro. Biochem Pharmacol. 78:941–950. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Song W, Pu J and He B: Tanshinol protects human umbilical vein endothelial cells against hydrogen peroxide‑induced apoptosis. Mol Med Rep 10: 2764-2770, 2014.
APA
Song, W., Pu, J., & He, B. (2014). Tanshinol protects human umbilical vein endothelial cells against hydrogen peroxide‑induced apoptosis. Molecular Medicine Reports, 10, 2764-2770. https://doi.org/10.3892/mmr.2014.2541
MLA
Song, W., Pu, J., He, B."Tanshinol protects human umbilical vein endothelial cells against hydrogen peroxide‑induced apoptosis". Molecular Medicine Reports 10.5 (2014): 2764-2770.
Chicago
Song, W., Pu, J., He, B."Tanshinol protects human umbilical vein endothelial cells against hydrogen peroxide‑induced apoptosis". Molecular Medicine Reports 10, no. 5 (2014): 2764-2770. https://doi.org/10.3892/mmr.2014.2541
Copy and paste a formatted citation
x
Spandidos Publications style
Song W, Pu J and He B: Tanshinol protects human umbilical vein endothelial cells against hydrogen peroxide‑induced apoptosis. Mol Med Rep 10: 2764-2770, 2014.
APA
Song, W., Pu, J., & He, B. (2014). Tanshinol protects human umbilical vein endothelial cells against hydrogen peroxide‑induced apoptosis. Molecular Medicine Reports, 10, 2764-2770. https://doi.org/10.3892/mmr.2014.2541
MLA
Song, W., Pu, J., He, B."Tanshinol protects human umbilical vein endothelial cells against hydrogen peroxide‑induced apoptosis". Molecular Medicine Reports 10.5 (2014): 2764-2770.
Chicago
Song, W., Pu, J., He, B."Tanshinol protects human umbilical vein endothelial cells against hydrogen peroxide‑induced apoptosis". Molecular Medicine Reports 10, no. 5 (2014): 2764-2770. https://doi.org/10.3892/mmr.2014.2541
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