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Article

Knockdown of Y‑box‑binding protein‑1 inhibits the malignant progression of HT‑29 colorectal adenocarcinoma cells by reversing epithelial‑mesenchymal transition

  • Authors:
    • Xue‑Bing Yan
    • Qing‑Chao Zhu
    • Hong‑Qi Chen
    • Jia‑Yuan Peng
    • Hong‑Lei  Chao
    • Hang‑Xiang Du
    • Zhi‑Gang Wang
    • Zhi‑Ming Jin
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200233, P.R. China
  • Pages: 2720-2728
    |
    Published online on: September 5, 2014
       https://doi.org/10.3892/mmr.2014.2545
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Abstract

Y‑box binding protein‑1 (YB‑1) has been identified as an oncoprotein in various malignancies. The aim of this study was to investigate the biological role of YB‑1 and its association with epithelial‑to‑mesenchymal transition (EMT) in colorectal cancer (CRC). The expression of YB‑1 and three EMT‑related proteins (E‑cadherin, N‑cadherin and vimentin) was analyzed in 80 CRC and matched normal tissue samples, by immunohistochemistry. The results indicated that the expression of YB‑1 was higher in CRC tissue samples than that in matched normal controls and was significantly correlated with tumor differentiation, tumor invasion, lymph node metastasis and distant metastases. Furthermore, analysis showed that YB‑1 expression was negatively correlated with E‑cadherin and positively correlated with N‑cadherin and vimentin expression. In vitro assays showed that knockdown of YB‑1 inhibited the proliferation, apoptosis resistance, invasion and migration of the HT‑29 CRC cell line. Of note, following knockdown of YB‑1, E‑cadherin expression was elevated whereas N‑cadherin and vimentin expression was reduced. Taken together, these results suggest that YB‑1 promotes the malignant progression of CRC in part through the induction of EMT, and YB‑1 may therefore be a potential novel target for CRC treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Yan XB, Zhu QC, Chen HQ, Peng JY, Chao HL, Du HX, Wang ZG and Jin ZM: Knockdown of Y‑box‑binding protein‑1 inhibits the malignant progression of HT‑29 colorectal adenocarcinoma cells by reversing epithelial‑mesenchymal transition. Mol Med Rep 10: 2720-2728, 2014.
APA
Yan, X., Zhu, Q., Chen, H., Peng, J., Chao, H., Du, H. ... Jin, Z. (2014). Knockdown of Y‑box‑binding protein‑1 inhibits the malignant progression of HT‑29 colorectal adenocarcinoma cells by reversing epithelial‑mesenchymal transition. Molecular Medicine Reports, 10, 2720-2728. https://doi.org/10.3892/mmr.2014.2545
MLA
Yan, X., Zhu, Q., Chen, H., Peng, J., Chao, H., Du, H., Wang, Z., Jin, Z."Knockdown of Y‑box‑binding protein‑1 inhibits the malignant progression of HT‑29 colorectal adenocarcinoma cells by reversing epithelial‑mesenchymal transition". Molecular Medicine Reports 10.5 (2014): 2720-2728.
Chicago
Yan, X., Zhu, Q., Chen, H., Peng, J., Chao, H., Du, H., Wang, Z., Jin, Z."Knockdown of Y‑box‑binding protein‑1 inhibits the malignant progression of HT‑29 colorectal adenocarcinoma cells by reversing epithelial‑mesenchymal transition". Molecular Medicine Reports 10, no. 5 (2014): 2720-2728. https://doi.org/10.3892/mmr.2014.2545
Copy and paste a formatted citation
x
Spandidos Publications style
Yan XB, Zhu QC, Chen HQ, Peng JY, Chao HL, Du HX, Wang ZG and Jin ZM: Knockdown of Y‑box‑binding protein‑1 inhibits the malignant progression of HT‑29 colorectal adenocarcinoma cells by reversing epithelial‑mesenchymal transition. Mol Med Rep 10: 2720-2728, 2014.
APA
Yan, X., Zhu, Q., Chen, H., Peng, J., Chao, H., Du, H. ... Jin, Z. (2014). Knockdown of Y‑box‑binding protein‑1 inhibits the malignant progression of HT‑29 colorectal adenocarcinoma cells by reversing epithelial‑mesenchymal transition. Molecular Medicine Reports, 10, 2720-2728. https://doi.org/10.3892/mmr.2014.2545
MLA
Yan, X., Zhu, Q., Chen, H., Peng, J., Chao, H., Du, H., Wang, Z., Jin, Z."Knockdown of Y‑box‑binding protein‑1 inhibits the malignant progression of HT‑29 colorectal adenocarcinoma cells by reversing epithelial‑mesenchymal transition". Molecular Medicine Reports 10.5 (2014): 2720-2728.
Chicago
Yan, X., Zhu, Q., Chen, H., Peng, J., Chao, H., Du, H., Wang, Z., Jin, Z."Knockdown of Y‑box‑binding protein‑1 inhibits the malignant progression of HT‑29 colorectal adenocarcinoma cells by reversing epithelial‑mesenchymal transition". Molecular Medicine Reports 10, no. 5 (2014): 2720-2728. https://doi.org/10.3892/mmr.2014.2545
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