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Article

Methanol extracts of Euphorbia cooperi inhibit the production of inflammatory mediators by inhibiting the activation of c‑Jun N‑terminal kinase and p38 in murine macrophages

  • Authors:
    • Young‑Chang Cho
    • In‑Seon Lee
    • Huiyun Seo
    • Anna Ju
    • Deokkyu  Youn
    • Younghyun Kim
    • Jaehee Choun
    • Sayeon Cho
  • View Affiliations / Copyright

    Affiliations: College of Pharmacy, Chung‑Ang University, Seoul 156-756, Republic of Korea
  • Pages: 2663-2668
    |
    Published online on: September 11, 2014
       https://doi.org/10.3892/mmr.2014.2560
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Abstract

Numerous Euphorbiaceae plants have been used for the treatment of diseases, including liver diseases, asthma and rheumatism. The present study evaluated the effect of methanol extracts from Euphorbia cooperi (MEC), a member of the Euphorbiaceae plant family, on the production of inflammatory cytokines interleukin (IL)‑6 and tumor necrosis factor (TNF)‑α, nitric oxide (NO) as well as the activation of mitogen‑activated protein kinase and nuclear factor (NF)‑κB signaling. Non‑cytotoxic concentrations of MEC significantly reduced the production of NO and IL‑6, but not TNF‑α, in lipopolysaccharide (LPS)‑stimulated RAW 264.7 macrophages. The decreased production of NO by MEC was due to alleviated expression of inducible NO synthase. Reporter assays with cells treated with MEC demonstrated reduced activator protein‑1 (AP-1) activity, while NF‑κB activity was not reduced. Furthermore, the phosphorylation levels of c‑Jun N‑terminal kinase (JNK) and p38 were suppressed by MEC while phosphorylation levels of inhibitor of κB were not reduced by MEC, suggesting that MEC‑mediated inactivation of JNK and p38 is the underlying regulatory mechanism for inflammatory mediators in LPS‑stimulated RAW 264.7 macrophages.
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1 

Fujiwara N and Kobayashi K: Macrophages in inflammation. Curr Drug Targets Inflamm Allergy. 4:281–286. 2005. View Article : Google Scholar

2 

Schroder K, Sweet MJ and Hume DA: Signal integration between IFNgamma and TLR signalling pathways in macrophages. Immunobiology. 211:511–524. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Aderem A and Underhill DM: Mechanisms of phagocytosis in macrophages. Annu Rev Immunol. 17:593–623. 1999. View Article : Google Scholar

4 

Aderem A and Ulevitch RJ: Toll-like receptors in the induction of the innate immune response. Nature. 406:782–787. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Cario E, Rosenberg IM, Brandwein SL, Beck PL, Reinecker HC and Podolsky DK: Lipopolysaccharide activates distinct signaling pathways in intestinal epithelial cell lines expressing Toll-like receptors. J Immunol. 164:966–972. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Fujihara M, Muroi M, Tanamoto K, Suzuki T, Azuma H and Ikeda H: Molecular mechanisms of macrophage activation and deactivation by lipopolysaccharide: roles of the receptor complex. Pharmacol Ther. 100:171–194. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Hanada T and Yoshimura A: Regulation of cytokine signaling and inflammation. Cytokine Growth Factor Rev. 13:413–421. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Nathan C and Xie QW: Regulation of biosynthesis of nitric oxide. J Biol Chem. 269:13725–13728. 1994.PubMed/NCBI

9 

MacMicking J, Xie QW and Nathan C: Nitric oxide and macrophage function. Annu Rev Immunol. 15:323–350. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Clancy RM, Amin AR and Abramson SB: The role of nitric oxide in inflammation and immunity. Arthritis Rheum. 41:1141–1151. 1998. View Article : Google Scholar : PubMed/NCBI

11 

Moncada S: Nitric oxide: discovery and impact on clinical medicine. J R Soc Med. 92:164–169. 1999.PubMed/NCBI

12 

Dinarello CA: Anti-inflammatory agents: present and future. Cell. 140:935–950. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Eke T, Al-Husainy S and Raynor MK: The spectrum of ocular inflammation caused by Euphorbia plant sap. Arch Ophthalmol. 18:13–16. 2000. View Article : Google Scholar

14 

Lazarini CA, Uema AH, Brandão GM, Guimarães AP and Bernardi MM: Croton zehntneri essential oil: effects on behavioral models related to depression and anxiety. Phytomedicine. 7:477–481. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Thyagarajan SP, Subramanian S, Thirunalasundari T, Venkateswaran PS and Blumberg BS: Effect of Phyllanthus amarus on chronic carriers of hepatitis B virus. Lancet. 1:764–766. 1988. View Article : Google Scholar : PubMed/NCBI

16 

Suarez AI, Compagnone RS, Salazar-Bookaman MM, et al: Antinociceptive and anti-inflammatory effects of Croton malambo bark aqueous extract. J Ethnopharmacol. 88:11–14. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Pereira U, Garcia-Le Gal C, Le Gal G, et al: Effects of sangre de drago in an in vitro model of cutaneous neurogenic inflammation. Exp Dermatol. 19:796–799. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Risco E, Ghia F, Vila R, Iglesias J, Alvarez E and Cañigueral S: Immunomodulatory activity and chemical characterisation of sangre de drago (dragon’s blood) from Croton lechleri. Planta Med. 69:785–794. 2003.PubMed/NCBI

19 

Nathan C and Xie QW: Nitric oxide synthases: roles, tolls, and controls. Cell. 78:915–918. 1994. View Article : Google Scholar : PubMed/NCBI

20 

Szabo C and Thiemermann C: Regulation of the expression of the inducible isoform of nitric oxide synthase. Adv Pharmacol. 34:113–153. 1995. View Article : Google Scholar : PubMed/NCBI

21 

Bosca L, Zeini M, Traves PG and Hortelano S: Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. Toxicology. 208:249–258. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Kröncke KD, Fehsel K and Kolb-Bachofen V: Inducible nitric oxide synthase in human diseases. Clin Exp Immunol. 113:147–156. 1998.

23 

Guadagni F, Ferroni P, Palmirotta R, Portarena I, Formica V and Roselli M: Review. TNF/VEGF cross-talk in chronic inflammation-related cancer initiation and progression: an early target in anticancer therapeutic strategy. In Vivo. 21:147–161. 2007.PubMed/NCBI

24 

Nishimoto N and Kishimoto T: Interleukin 6: from bench to bedside. Nat Clin Pract Rheumatol. 2:619–626. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Kavanaugh A: Interleukin-6 inhibition and clinical efficacy in rheumatoid arthritis treatment - data from randomized clinical trials. Bull NYU Hosp Jt Dis. 65:S16–S20. 2007.PubMed/NCBI

26 

Straub RH, Harle P, Yamana S, et al: Anti-interleukin-6 receptor antibody therapy favors adrenal androgen secretion in patients with rheumatoid arthritis: a randomized, double-blind, placebo-controlled study. Arthritis Rheum. 54:1778–1785. 2006. View Article : Google Scholar

27 

Samavati L, Rastogi R, Du W, Huttemann M, Fite A and Franchi L: STAT3 tyrosine phosphorylation is critical for interleukin 1 beta and interleukin-6 production in response to lipopolysaccharide and live bacteria. Mol Immunol. 46:1867–1877. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Zhu ZG, Jin H, Yu PJ, Tian YX, Zhang JJ and Wu SG: Mollugin inhibits the inflammatory response in lipopolysaccharide-stimulated RAW264.7 macrophages by blocking the janus kinase-signal transducers and activators of transcription signaling pathway. Biol Pharm Bull. 36:399–406. 2013. View Article : Google Scholar

29 

Lee C, Lim HK, Sakong J, Lee YS, Kim JR and Baek SH: Janus kinase-signal transducer and activator of transcription mediates phosphatidic acid-induced interleukin (IL)-1beta and IL-6 production. Mol Pharmacol. 69:1041–1047. 2006.PubMed/NCBI

30 

Stalinska K, Guzdek A, Rokicki M and Koj A: Transcription factors as targets of the anti-inflammatory treatment. A cell culture study with extracts from some Mediterranean diet plants. J Physiol Pharmaco. 56:S157–S169. 2005.

31 

Burk DR, Senechal-Willis P, Lopez LC, Hogue BG and Daskalova SM: Suppression of lipopolysaccharide-induced inflammatory responses in RAW 264.7 murine macrophages by aqueous extract of Clinopodium vulgare L. (Lamiaceae). J Ethnopharmacol. 126:397–405. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Liu H, Bargouti M, Zughaier S, et al: Osteoinductive LIM mineralization protein-1 suppresses activation of NF-kappaB and selectively regulates MAPK pathways in pre-osteoclasts. Bone. 46:1328–1335. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Shan J, Fu J, Zhao Z, et al: Chlorogenic acid inhibits lipopolysaccharide-induced cyclooxygenase-2 expression in RAW264.7 cells through suppressing NF-kappaB and JNK/AP-1 activation. Int Immunopharmacol. 9:1042–1048. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Watters JJ, Sommer JA, Pfeiffer ZA, Prabhu U, Guerra AN and Bertics PJ: A differential role for the mitogen-activated protein kinases in lipopolysaccharide signaling: the MEK/ERK pathway is not essential for nitric oxide and interleukin 1beta production. J Biol Chem. 277:9077–9087. 2002. View Article : Google Scholar

35 

Karin M and Ben-Neriah Y: Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity. Annu Rev Immunol. 18:621–663. 2000.PubMed/NCBI

36 

Karin M and Delhase M: The I kappa B kinase (IKK) and NF-kappa B: key elements of proinflammatory signalling. Semin Immunol. 12:85–98. 2000. View Article : Google Scholar : PubMed/NCBI

37 

Surh YJ, Chun KS, Cha HH, et al: Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Mutat Res. 480–481:243–268. 2001.

38 

Verma IM, Stevenson JK, Schwarz EM, Van Antwerp D and Miyamoto S: Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev. 9:2723–2735. 1995. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cho YC, Lee IS, Seo H, Ju A, Youn D, Kim Y, Choun J and Cho S: Methanol extracts of Euphorbia cooperi inhibit the production of inflammatory mediators by inhibiting the activation of c‑Jun N‑terminal kinase and p38 in murine macrophages. Mol Med Rep 10: 2663-2668, 2014.
APA
Cho, Y., Lee, I., Seo, H., Ju, A., Youn, D., Kim, Y. ... Cho, S. (2014). Methanol extracts of Euphorbia cooperi inhibit the production of inflammatory mediators by inhibiting the activation of c‑Jun N‑terminal kinase and p38 in murine macrophages. Molecular Medicine Reports, 10, 2663-2668. https://doi.org/10.3892/mmr.2014.2560
MLA
Cho, Y., Lee, I., Seo, H., Ju, A., Youn, D., Kim, Y., Choun, J., Cho, S."Methanol extracts of Euphorbia cooperi inhibit the production of inflammatory mediators by inhibiting the activation of c‑Jun N‑terminal kinase and p38 in murine macrophages". Molecular Medicine Reports 10.5 (2014): 2663-2668.
Chicago
Cho, Y., Lee, I., Seo, H., Ju, A., Youn, D., Kim, Y., Choun, J., Cho, S."Methanol extracts of Euphorbia cooperi inhibit the production of inflammatory mediators by inhibiting the activation of c‑Jun N‑terminal kinase and p38 in murine macrophages". Molecular Medicine Reports 10, no. 5 (2014): 2663-2668. https://doi.org/10.3892/mmr.2014.2560
Copy and paste a formatted citation
x
Spandidos Publications style
Cho YC, Lee IS, Seo H, Ju A, Youn D, Kim Y, Choun J and Cho S: Methanol extracts of Euphorbia cooperi inhibit the production of inflammatory mediators by inhibiting the activation of c‑Jun N‑terminal kinase and p38 in murine macrophages. Mol Med Rep 10: 2663-2668, 2014.
APA
Cho, Y., Lee, I., Seo, H., Ju, A., Youn, D., Kim, Y. ... Cho, S. (2014). Methanol extracts of Euphorbia cooperi inhibit the production of inflammatory mediators by inhibiting the activation of c‑Jun N‑terminal kinase and p38 in murine macrophages. Molecular Medicine Reports, 10, 2663-2668. https://doi.org/10.3892/mmr.2014.2560
MLA
Cho, Y., Lee, I., Seo, H., Ju, A., Youn, D., Kim, Y., Choun, J., Cho, S."Methanol extracts of Euphorbia cooperi inhibit the production of inflammatory mediators by inhibiting the activation of c‑Jun N‑terminal kinase and p38 in murine macrophages". Molecular Medicine Reports 10.5 (2014): 2663-2668.
Chicago
Cho, Y., Lee, I., Seo, H., Ju, A., Youn, D., Kim, Y., Choun, J., Cho, S."Methanol extracts of Euphorbia cooperi inhibit the production of inflammatory mediators by inhibiting the activation of c‑Jun N‑terminal kinase and p38 in murine macrophages". Molecular Medicine Reports 10, no. 5 (2014): 2663-2668. https://doi.org/10.3892/mmr.2014.2560
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