Phenethyl isothiocyanate suppresses cervical carcinoma metastasis potential and its molecular mechanism

Zhang,Li; Hao,Quan; Bao,Lewen; Liu,Wenxin; Fu,Xin; Chen,Ying; Wu,Huijuan

doi:10.3892/mmr.2014.2565

November-2014 Volume 10 Issue 5

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November-2014 Volume 10 Issue 5

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Article

Phenethyl isothiocyanate suppresses cervical carcinoma metastasis potential and its molecular mechanism

Authors:
- Li Zhang
- Quan Hao
- Lewen Bao
- Wenxin Liu
- Xin Fu
- Ying Chen
- Huijuan Wu
View Affiliations / Copyright

Affiliations: Department of Gynecologic Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, P.R. China
Pages: 2675-2680
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Published online on: September 12, 2014

https://doi.org/10.3892/mmr.2014.2565
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Abstract

Tumor metastasis is a prominent cause of treatment failure in cervical carcinoma. Phenethyl isothiocyanate (PEITC) is an active component extracted from cruciferous plants that has exhibited anticancer activity in various types of human cancer; however, its effect on the inhibition of metastasis remains unclear. The current study aimed to explore the effect of PEITC on the suppression of metastasis in HeLa cervical carcinoma cells. Multiple variables were assessed with different methods as follows: Cell viability, with a Vi‑CELL analyzer; cell adhesion, by MTS assay; cell invasion, by Transwell assay; cell cycle, by flow cytometry assay; cytokine concentration, by ELISA assay; metastasis‑related gene and protein expression, by quantitative polymerase chain reaction and western blotting; and transcription factor activity, by gene reporter assay. The results indicated that PEITC exhibited an inhibitory effect on the adhesion and invasion of HeLa cells by induction of G2/M phase arrest, it reduced the expression of CDK1, MMP‑2/9, CD44, ICAM‑1, increased the production of TGF‑β, IL‑6 and IL‑8, and increased the phosphorylation of Smad2. These results suggest that PEITC may be a potential antitumor compound, acting through the TGF‑β/Smad2 pathway; and it has the potential for future use as a therapy for cervical carcinoma subsequent to further studies.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Phenethyl isothiocyanate suppresses cervical carcinoma metastasis potential and its molecular mechanism

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