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Critical role of insulin‑like growth factor binding protein‑5 in methamphetamine‑induced apoptosis in cardiomyocytes

  • Authors:
    • Ka‑Pui Leung
    • Yi‑Hong Qu
    • Dong‑Fang Qiao
    • Wei‑Bing Xie
    • Dong‑Ri Li
    • Jing‑Tao Xu
    • Hui‑Jun Wang
    • Xia  Yue
  • View Affiliations / Copyright

    Affiliations: Department of Forensic Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
    Copyright: © Leung et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2306-2312
    |
    Published online on: September 16, 2014
       https://doi.org/10.3892/mmr.2014.2572
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Abstract

Methamphetamine (MA) is a highly abused amphetamine‑like psychostimulant. At present, the mechanisms underlying MA‑induced cardiotoxicity are poorly understood. The cardiotoxic effects have yet not been clearly elucidated with respect to the apoptotic pathway. Insulin‑like growth factor binding protein‑5 (IGFBP5) is important for cell growth control and the induction of apoptosis. The aim of the present study was to analyze whether IGFBP5 is involved in MA‑induced apoptosis as a novel target. MA‑induced apoptosis was observed in neonatal rat ventricular myocytes (NRVMs) in a concentration‑dependent manner using a terminal deoxyribonucleotide transferase‑mediated dUTP nick end‑labeling assay. Using reverse transcription polymerase chain reaction and western blotting, MA was demonstrated to induce concentration‑dependent increases in the expression of IGFBP5. Silencing IGFBP5 with small interfering RNA significantly reduced apoptosis and suppressed the expression of caspase‑3 in NRVMs following treatment with MA. To the best of our knowledge, the present study provided the first evidence suggesting that IGFBP5 is a potential therapeutic target in MA‑induced apoptosis in vitro, providing a foundation for future in vivo studies.
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Copy and paste a formatted citation
Spandidos Publications style
Leung KP, Qu YH, Qiao DF, Xie WB, Li DR, Xu JT, Wang HJ and Yue X: Critical role of insulin‑like growth factor binding protein‑5 in methamphetamine‑induced apoptosis in cardiomyocytes. Mol Med Rep 10: 2306-2312, 2014.
APA
Leung, K., Qu, Y., Qiao, D., Xie, W., Li, D., Xu, J. ... Yue, X. (2014). Critical role of insulin‑like growth factor binding protein‑5 in methamphetamine‑induced apoptosis in cardiomyocytes. Molecular Medicine Reports, 10, 2306-2312. https://doi.org/10.3892/mmr.2014.2572
MLA
Leung, K., Qu, Y., Qiao, D., Xie, W., Li, D., Xu, J., Wang, H., Yue, X."Critical role of insulin‑like growth factor binding protein‑5 in methamphetamine‑induced apoptosis in cardiomyocytes". Molecular Medicine Reports 10.5 (2014): 2306-2312.
Chicago
Leung, K., Qu, Y., Qiao, D., Xie, W., Li, D., Xu, J., Wang, H., Yue, X."Critical role of insulin‑like growth factor binding protein‑5 in methamphetamine‑induced apoptosis in cardiomyocytes". Molecular Medicine Reports 10, no. 5 (2014): 2306-2312. https://doi.org/10.3892/mmr.2014.2572
Copy and paste a formatted citation
x
Spandidos Publications style
Leung KP, Qu YH, Qiao DF, Xie WB, Li DR, Xu JT, Wang HJ and Yue X: Critical role of insulin‑like growth factor binding protein‑5 in methamphetamine‑induced apoptosis in cardiomyocytes. Mol Med Rep 10: 2306-2312, 2014.
APA
Leung, K., Qu, Y., Qiao, D., Xie, W., Li, D., Xu, J. ... Yue, X. (2014). Critical role of insulin‑like growth factor binding protein‑5 in methamphetamine‑induced apoptosis in cardiomyocytes. Molecular Medicine Reports, 10, 2306-2312. https://doi.org/10.3892/mmr.2014.2572
MLA
Leung, K., Qu, Y., Qiao, D., Xie, W., Li, D., Xu, J., Wang, H., Yue, X."Critical role of insulin‑like growth factor binding protein‑5 in methamphetamine‑induced apoptosis in cardiomyocytes". Molecular Medicine Reports 10.5 (2014): 2306-2312.
Chicago
Leung, K., Qu, Y., Qiao, D., Xie, W., Li, D., Xu, J., Wang, H., Yue, X."Critical role of insulin‑like growth factor binding protein‑5 in methamphetamine‑induced apoptosis in cardiomyocytes". Molecular Medicine Reports 10, no. 5 (2014): 2306-2312. https://doi.org/10.3892/mmr.2014.2572
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