Open Access

Increased production of suilysin contributes to invasive infection of the Streptococcus suis strain 05ZYH33

  • Authors:
    • Zhengxin He
    • Yaya Pian
    • Zhiqiang Ren
    • Lili Bi
    • Yuan Yuan
    • Yuling Zheng
    • Yongqiang Jiang
    • Fukun Wang
  • View Affiliations

  • Published online on: September 22, 2014     https://doi.org/10.3892/mmr.2014.2586
  • Pages: 2819-2826
  • Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Streptococcus suis serotype 2 (SS2) is widely recognized in the veterinary world as the cause of rapidly progressive and fatal sepsis in infant pigs, manifested with meningitis, polyarthritis and pneumonia. It has evolved into a highly infectious strain, and caused two large-scale outbreaks of human epidemic in China, characterized bytypical toxic-shock syndrome and invasive infection. However, the molecular basis of virulence of this emerging zoonotic pathogen is still largely unknown. The present study shows that the sequence type (ST)7 epidemic strain S. suis 05ZYH33 causes higher mortality, higher necrosis of polymorphonuclear neutrophils and a significantly higher damage to human umbilical vein endothelial cells compared to the non-epidemic strain S. suis 1940. These differences appear to associate with the enhanced secretion of suilysin (sly) by S. suis 05ZYH33 compared to the non-epidemic strain 1940. Inclusion of additional strains confirmed that the epidemic ST7 strains produce more sly protein (mean, 1.49 g/ml; range, 0.76‑1.91 g/ml) than non‑epidemic strains (mean, 0.33 g/ml; range, 0.07-0.94 g/ml), and this difference is significant (P<0.001). The nonpolar mutant strain S. suis Δsly, constructed from the epidemic ST7 strain S. suis 05ZYH33 confirmed the role of sly on the enhanced virulence of S. suis ST7 strains. These findings suggest that increased sly production in S. suis 05ZYH33 facilitates penetration to the epithelium and its survival in the bloodstream, thereby contributing to the invasive infection.
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December-2014
Volume 10 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
He Z, Pian Y, Ren Z, Bi L, Yuan Y, Zheng Y, Jiang Y and Wang F: Increased production of suilysin contributes to invasive infection of the Streptococcus suis strain 05ZYH33. Mol Med Rep 10: 2819-2826, 2014
APA
He, Z., Pian, Y., Ren, Z., Bi, L., Yuan, Y., Zheng, Y. ... Wang, F. (2014). Increased production of suilysin contributes to invasive infection of the Streptococcus suis strain 05ZYH33. Molecular Medicine Reports, 10, 2819-2826. https://doi.org/10.3892/mmr.2014.2586
MLA
He, Z., Pian, Y., Ren, Z., Bi, L., Yuan, Y., Zheng, Y., Jiang, Y., Wang, F."Increased production of suilysin contributes to invasive infection of the Streptococcus suis strain 05ZYH33". Molecular Medicine Reports 10.6 (2014): 2819-2826.
Chicago
He, Z., Pian, Y., Ren, Z., Bi, L., Yuan, Y., Zheng, Y., Jiang, Y., Wang, F."Increased production of suilysin contributes to invasive infection of the Streptococcus suis strain 05ZYH33". Molecular Medicine Reports 10, no. 6 (2014): 2819-2826. https://doi.org/10.3892/mmr.2014.2586