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Inducing human parthenogenetic embryonic stem cells into islet‑like clusters

  • Authors:
    • Jin Li
    • Jingjing He
    • Ge Lin
    • Guangxiu Lu
  • View Affiliations / Copyright

    Affiliations: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, Hunan 410078, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2882-2890
    |
    Published online on: September 22, 2014
       https://doi.org/10.3892/mmr.2014.2588
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Abstract

In order to determine whether human parthenogenetic embryonic stem (hpES) cells have the potential to differentiate into functional cells, a modified four‑step protocol was used to induce the hpES cells into islet‑like clusters (ILCs) in vitro. Growth factors activin A, retinoic acid, nicotinamide, Exendin‑4 and betacellulin were added sequentially to the hpES cells at each step. The terminally differentiated cells were shown to gather into ILCs. Immunohistochemistry and semi quantitative polymerase chain reaction analyses demonstrated that the ILCs expressed islet specific hormones and functional markers. Furthermore, an insulin release test indicated that the clusters had the same physiological function as islets. The ILCs derived from hpES cells shared similar characteristics with islets. These results indicate that hpES cell‑derived ILCs may be used as reliable material for the treatment of type I diabetes mellitus.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, He J, Lin G and Lu G: Inducing human parthenogenetic embryonic stem cells into islet‑like clusters. Mol Med Rep 10: 2882-2890, 2014.
APA
Li, J., He, J., Lin, G., & Lu, G. (2014). Inducing human parthenogenetic embryonic stem cells into islet‑like clusters. Molecular Medicine Reports, 10, 2882-2890. https://doi.org/10.3892/mmr.2014.2588
MLA
Li, J., He, J., Lin, G., Lu, G."Inducing human parthenogenetic embryonic stem cells into islet‑like clusters". Molecular Medicine Reports 10.6 (2014): 2882-2890.
Chicago
Li, J., He, J., Lin, G., Lu, G."Inducing human parthenogenetic embryonic stem cells into islet‑like clusters". Molecular Medicine Reports 10, no. 6 (2014): 2882-2890. https://doi.org/10.3892/mmr.2014.2588
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, He J, Lin G and Lu G: Inducing human parthenogenetic embryonic stem cells into islet‑like clusters. Mol Med Rep 10: 2882-2890, 2014.
APA
Li, J., He, J., Lin, G., & Lu, G. (2014). Inducing human parthenogenetic embryonic stem cells into islet‑like clusters. Molecular Medicine Reports, 10, 2882-2890. https://doi.org/10.3892/mmr.2014.2588
MLA
Li, J., He, J., Lin, G., Lu, G."Inducing human parthenogenetic embryonic stem cells into islet‑like clusters". Molecular Medicine Reports 10.6 (2014): 2882-2890.
Chicago
Li, J., He, J., Lin, G., Lu, G."Inducing human parthenogenetic embryonic stem cells into islet‑like clusters". Molecular Medicine Reports 10, no. 6 (2014): 2882-2890. https://doi.org/10.3892/mmr.2014.2588
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