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Article

Serum amyloid A promotes osteosarcoma invasion via upregulating αvβ3 integrin

  • Authors:
    • Peng Ren
    • Deshun Sun
    • Dajiang Xin
    • Wanli Ma
    • Peng Chen
    • Hongwei Gao
    • Shouqiang Zhang
    • Mingzhi Gong
  • View Affiliations / Copyright

    Affiliations: Department of Osteology, The Second Hospital, Shandong University, Jinan, Shandong 250033, P.R. China, Department of Osteology, Zhangqiu City Hospital of Traditional Chinese Medicine, Jinan, Shandong 250200, P.R. China, Department of Osteology, Mount Yantai Hospital, Yantai, Shandong 264000, P.R. China
  • Pages: 3106-3112
    |
    Published online on: October 14, 2014
       https://doi.org/10.3892/mmr.2014.2635
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Abstract

Serum amyloid A (SAA) is regarded as an important acute phase protein involved in tumor progression and metastasis. However, at present there is no evidence of its involvement in osteosarcoma. The present study aimed to investigate the effect of SAA on the invasion of osteosarcoma cells. The effects of SAA on the migration and invasion of osteosarcoma cells were detected using scratch wound healing and transwell assays, respectively. The expression of αvβ3 integrin was detected at the protein and mRNA levels in U2OS cells. Agonists, inhibitors or siRNA of formyl peptide receptor like‑1 (FPRL‑1), mitogen‑activated protein kinases and αvβ3 integrin were used to investigate the mechanism underlying the effects of SAA on the regulation of U2OS cell migration and invasion. The present study revealed that SAA promoted osteosarcoma cell migration and invasion. SAA upregulated the expression of αvβ3 integrin in a concentration‑ and time‑dependent manner. When inhibiting αvβ3 integrin with its antagonist, the migration and invasion abilities of the U2OS cells were markedly inhibited. SAA‑induced αvβ3 integrin production was significantly downregulated by inhibiting FPRL‑1 with siRNA and inhibitors. The present study also found that extracellular signal‑regulated kinase (ERK) 1/2, but not c‑Jun N‑terminal kinase or p38, was important in this process. These findings demonstrated that SAA regulated osteosarcoma cell migration and invasion via the FPRL‑1/ERK/αvβ3 integrin pathway.
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1 

Murphey MD, Robbin MR, McRae GA, Flemming DJ, Temple HT and Kransdorf MJ: The many faces of osteosarcoma. Radiographics. 17:1205–1231. 1997. View Article : Google Scholar : PubMed/NCBI

2 

Allison DC, Carney SC, Ahlmann ER, Hendifar A, Chawla S, Fedenko A, et al: A meta-analysis of osteosarcoma outcomes in the modern medical era. Sarcoma. 2012:7048722012. View Article : Google Scholar : PubMed/NCBI

3 

Mirabello L, Troisi RJ and Savage SA: Osteosarcoma incidence and survival rates from 1973 to 2004: data from the Surveillance, Epidemiology, and End Results Program. Cancer. 115:1531–1543. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Assi H, Missenard G, Terrier P, Le Pechoux C, Bonvalot S, Vanel D, et al: Intensive induction chemotherapy without methotrexate in adult patients with localized osteosarcoma: results of the Institut Gustave-Roussy phase II trial. Curr Oncol. 17:23–31. 2010. View Article : Google Scholar

5 

Balkwill F and Mantovani A: Inflammation and cancer: back to Virchow? Lancet. 357:539–545. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Coussens LM and Werb Z: Inflammation and cancer. Nature. 420:860–867. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Mantovani A, Allavena P, Sica A and Balkwill F: Cancer-related inflammation. Nature. 454:436–444. 2008. View Article : Google Scholar

8 

Cole SW: Chronic inflammation and breast cancer recurrence. Journal Clinical Oncology. 27:3418–3419. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Crawford S: Anti-inflammatory/antioxidant use in long-term maintenance cancer therapy: A new therapeutic approach to disease progression and recurrence. Ther Adv Med Oncol. 6:52–68. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Noh K, Kim KO, Patel NR, Staples JR, Minematsu H, Nair K, et al: Targeting inflammatory kinase as an adjuvant treatment for osteosarcomas. J Bone Joint Surg Am. 93:723–732. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Liu C, Pan C, Shen J, Wang H and Yong L: Identification of serum amyloid A in the serum of gastric cancer patients by protein expression profiling. Oncol Lett. 3:1259–1262. 2012.PubMed/NCBI

12 

Mittal A, Poudel B, Pandeya DR, Gupta SP, Sathian B and Yadav K: Serum amyloid a as an independent prognostic factor for renal cell carcinoma - a hospital based study from the Western region of Nepal. Asian Pac J Cancer Prev. 13:2253–2255. 2012. View Article : Google Scholar

13 

Zhang G, Sun X, Lv H, Yang X and Kang X: Serum amyloid A: A new potential serum marker correlated with the stage of breast cancer. Oncol Lett. 3:940–944. 2012.PubMed/NCBI

14 

Desgrosellier JS and Cheresh DA: Integrins in cancer: biological implications and therapeutic opportunities. Nat Rev Cancer. 10:9–22. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Desai B, Rogers MJ and Chellaiah MA: Mechanisms of osteopontin and CD44 as metastatic principles in prostate cancer cells. Mol Cancer. 6:182007. View Article : Google Scholar

16 

Sung V, Stubbs JT 3rd, Fisher L, Aaron AD and Thompson EW: Bone sialoprotein supports breast cancer cell adhesion proliferation and migration through differential usage of the alpha(v)beta3 and alpha(v)beta5 integrins. J Cell Physiol. 176:482–494. 1998. View Article : Google Scholar

17 

Pecheur I, Peyruchaud O, Serre CM, Guglielmi J, Voland C, Bourre F, et al: Integrin alpha(v)beta3 expression confers on tumor cells a greater propensity to metastasize to bone. FASEB J. 16:1266–1268. 2002.PubMed/NCBI

18 

Tome Y, Kimura H, Maehara H, Sugimoto N, Bouvet M, Tsuchiya H, et al: High lung-metastatic variant of human osteosarcoma cells, selected by passage of lung metastasis in nude mice, is associated with increased expression of alphavbeta3 integrin. Anticancer Res. 33:3623–3627. 2013.

19 

Kurihara Y, Nakahara T and Furue M: alphaVbeta3-integrin expression through ERK activation mediates cell attachment and is necessary for production of tumor necrosis factor alpha in monocytic THP-1 cells stimulated by phorbol myristate acetate. Cell Immunol. 270:25–31. 2011. View Article : Google Scholar

20 

Furundzija V, Fritzsche J, Kaufmann J, Meyborg H, Fleck E, Kappert K, et al: IGF-1 increases macrophage motility via PKC/p38-dependent alphavbeta3-integrin inside-out signaling. Biochem Biophys Res Commun. 394:786–791. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Li B, Dong Z, Liu H, Xia YF, Liu XM, Luo BB, et al: Serum amyloid A stimulates lipoprotein-associated phospholipase A2 expression in vitro and in vivo. Atherosclerosis. 228:370–379. 2013. View Article : Google Scholar

22 

Dong Z, An F, Wu T, Zhang C, Zhang M, Zhang Y, et al: PTX3, a key component of innate immunity, is induced by SAA via FPRL1-mediated signaling in HAECs. J Cell Biochem. 112:2097–2105. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Levinson H, Hopper JE and Ehrlich HP: Overexpression of integrin alphav promotes human osteosarcoma cell populated collagen lattice contraction and cell migration. J Cell Physiol. 193:219–224. 2002. View Article : Google Scholar : PubMed/NCBI

24 

Uhlar CM and Whitehead AS: Serum amyloid A, the major vertebrate acute-phase reactant. Eur J Biochem. 265:501–523. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Tölle M, Huang T, Schuchardt M, Jankowski V, Prufer N, Jankowski J, et al: High-density lipoprotein loses its anti-inflammatory capacity by accumulation of pro-inflammatory-serum amyloid A. Cardiovasc Res. 94:154–162. 2012.PubMed/NCBI

26 

d’Eril GM, Anesi A, Maggiore M and Leoni V: Biological variation of serum amyloid A in healthy subjects. Clin Chem. 47:1498–1499. 2001.PubMed/NCBI

27 

Shattil SJ, Kim C and Ginsberg MH: The final steps of integrin activation: the end game. Nat Rev Mol Cell Biol. 11:288–300. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Duan X, Jia SF, Zhou Z, Langley RR, Bolontrade MF and Kleinerman ES: Association of alphavbeta3 integrin expression with the metastatic potential and migratory and chemotactic ability of human osteosarcoma cells. Clin Exp Metastasis. 21:747–753. 2004. View Article : Google Scholar

29 

Song C, Hsu K, Yamen E, Yan W, Fock J, Witting PK, et al: Serum amyloid A induction of cytokines in monocytes/macrophages and lymphocytes. Atherosclerosis. 207:374–383. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Pechkovsky DV, Scaffidi AK, Hackett TL, Ballard J, Shaheen F, Thompson PJ, et al: Transforming growth factor beta1 induces alphavbeta3 integrin expression in human lung fibroblasts via a beta3 integrin-, c-Src-, and p38 MAPK-dependent pathway. J Biol Chem. 283:12898–12908. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ren P, Sun D, Xin D, Ma W, Chen P, Gao H, Zhang S and Gong M: Serum amyloid A promotes osteosarcoma invasion via upregulating αvβ3 integrin. Mol Med Rep 10: 3106-3112, 2014.
APA
Ren, P., Sun, D., Xin, D., Ma, W., Chen, P., Gao, H. ... Gong, M. (2014). Serum amyloid A promotes osteosarcoma invasion via upregulating αvβ3 integrin. Molecular Medicine Reports, 10, 3106-3112. https://doi.org/10.3892/mmr.2014.2635
MLA
Ren, P., Sun, D., Xin, D., Ma, W., Chen, P., Gao, H., Zhang, S., Gong, M."Serum amyloid A promotes osteosarcoma invasion via upregulating αvβ3 integrin". Molecular Medicine Reports 10.6 (2014): 3106-3112.
Chicago
Ren, P., Sun, D., Xin, D., Ma, W., Chen, P., Gao, H., Zhang, S., Gong, M."Serum amyloid A promotes osteosarcoma invasion via upregulating αvβ3 integrin". Molecular Medicine Reports 10, no. 6 (2014): 3106-3112. https://doi.org/10.3892/mmr.2014.2635
Copy and paste a formatted citation
x
Spandidos Publications style
Ren P, Sun D, Xin D, Ma W, Chen P, Gao H, Zhang S and Gong M: Serum amyloid A promotes osteosarcoma invasion via upregulating αvβ3 integrin. Mol Med Rep 10: 3106-3112, 2014.
APA
Ren, P., Sun, D., Xin, D., Ma, W., Chen, P., Gao, H. ... Gong, M. (2014). Serum amyloid A promotes osteosarcoma invasion via upregulating αvβ3 integrin. Molecular Medicine Reports, 10, 3106-3112. https://doi.org/10.3892/mmr.2014.2635
MLA
Ren, P., Sun, D., Xin, D., Ma, W., Chen, P., Gao, H., Zhang, S., Gong, M."Serum amyloid A promotes osteosarcoma invasion via upregulating αvβ3 integrin". Molecular Medicine Reports 10.6 (2014): 3106-3112.
Chicago
Ren, P., Sun, D., Xin, D., Ma, W., Chen, P., Gao, H., Zhang, S., Gong, M."Serum amyloid A promotes osteosarcoma invasion via upregulating αvβ3 integrin". Molecular Medicine Reports 10, no. 6 (2014): 3106-3112. https://doi.org/10.3892/mmr.2014.2635
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