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Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats

  • Authors:
    • Qiongmei Guo
    • Xuefang Du
    • Yanli Zhao
    • Dong Zhang
    • Lihui Yue
    • Zhenxian Wang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China, Department of Anesthesiology, Xingtai Eye Hospital, Xingtai, Hebei 054001, P.R. China, Department of Urinary Surgery, Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2875-2881
    |
    Published online on: October 14, 2014
       https://doi.org/10.3892/mmr.2014.2641
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Abstract

Ischemic postconditioning (IPo) attenuates ischemia‑reperfusion injuries (IRI) in various organs, of both animals and humans. This study tested the hypothesis that IPo attenuates renal IRI through the upregulation of heat shock protein (HSP)70, HSP27 and heme oxygenase‑1 (HO‑1, also known as HSP 32) expression. Adult Sprague Dawley rats were subjected to bilateral renal ischemia for 45 min followed by reperfusion for up to 48 h. One group of rats received IPo prior to restoring full perfusion. Another group was administered 100 mg/kg HSP inhibitor quercetin, injected intraperitoneally 1 h prior to ischemia. Control rats received sham operations. Renal IR resulted in severe morphological and pathological changes, with increased serum creatinine and blood urea nitrogen concentrations. IR resulted in increased inflammation by inducing plasma tumor necrosis factor‑α and renal nuclear factor kappa‑light‑chain‑enhancer of activated B cells expression. IR also increased lipid peroxidation, as indicated by elevated malondialdehyde content, reduced superoxide dismutase activity and increased renal apoptosis. Renal HSP70, HSP27 and HO‑1 mRNA and protein levels were increased by IR and further elevated by IPo. IPo attenuated these changes observed in pathology, lipid peroxidation, apoptosis and inflammation. Quercetin treatment abolished all the protective effects of IPo. In conclusion, this study showed that IPo can attenuate lipid peroxidation, apoptosis and inflammation as well as renal IRI by upregulating the expression of HSP70, HSP27 and HO‑1.
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Copy and paste a formatted citation
Spandidos Publications style
Guo Q, Du X, Zhao Y, Zhang D, Yue L and Wang Z: Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats. Mol Med Rep 10: 2875-2881, 2014.
APA
Guo, Q., Du, X., Zhao, Y., Zhang, D., Yue, L., & Wang, Z. (2014). Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats. Molecular Medicine Reports, 10, 2875-2881. https://doi.org/10.3892/mmr.2014.2641
MLA
Guo, Q., Du, X., Zhao, Y., Zhang, D., Yue, L., Wang, Z."Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats". Molecular Medicine Reports 10.6 (2014): 2875-2881.
Chicago
Guo, Q., Du, X., Zhao, Y., Zhang, D., Yue, L., Wang, Z."Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats". Molecular Medicine Reports 10, no. 6 (2014): 2875-2881. https://doi.org/10.3892/mmr.2014.2641
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Q, Du X, Zhao Y, Zhang D, Yue L and Wang Z: Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats. Mol Med Rep 10: 2875-2881, 2014.
APA
Guo, Q., Du, X., Zhao, Y., Zhang, D., Yue, L., & Wang, Z. (2014). Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats. Molecular Medicine Reports, 10, 2875-2881. https://doi.org/10.3892/mmr.2014.2641
MLA
Guo, Q., Du, X., Zhao, Y., Zhang, D., Yue, L., Wang, Z."Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats". Molecular Medicine Reports 10.6 (2014): 2875-2881.
Chicago
Guo, Q., Du, X., Zhao, Y., Zhang, D., Yue, L., Wang, Z."Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats". Molecular Medicine Reports 10, no. 6 (2014): 2875-2881. https://doi.org/10.3892/mmr.2014.2641
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