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In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction

  • Authors:
    • Ping Hua
    • You‑Yu Wang
    • Li‑Bao Liu
    • Jia‑Liang Liu
    • Jian‑Yang Liu
    • Yan‑Qi Yang
    • Song‑Ran Yang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, The Sun Yat‑Sen Memorial Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China, Department of Thoracic Surgery, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China, Department of Cardiothoracic Surgery, The Sun Yat‑Sen Memorial Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China, Department of Neurology, Guangzhou First Municipal People's Hospital, Guangzhou, Guangdong 510180, P.R. China
    Copyright: © Hua et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 113-120
    |
    Published online on: October 15, 2014
       https://doi.org/10.3892/mmr.2014.2649
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Abstract

Superparamagnetic iron oxide (SPIO) nanoparticles generate superparamagnetism, thereby resulting in an inhomogeneous local magnetic field, which shortens the T2 value on magnetic resonance imaging (MRI). The purpose of the present study was to use MRI to track bone marrow mesenchymal stem cells (BMSCs) labeled with SPIO in a rat model of myocardial infarction. The BMSCs were isolated from rats and labeled with SPIO. The anterior descending branch of the coronary artery was ligated under anesthesia. Two weeks later, the rats received, at random, 5x107 SPIO‑labeled BMSCs, 5x107 unlabeled BMSCs or a vehicle (100 µl phosphate‑buffered saline) via direct injection into the ischemic area (20 animals/group). MRI was used to track the SPIO‑labeled BMSCs and the rats were then sacrificed to verify the presence of BMSCs using immunohistochemistry with an anti‑CD90 antibody. The procedure labeled 99% of the BMSCs with SPIO, which exhibited low‑intensity signals on T2 and T2* MRI imaging. At 24 h post‑BMSC transplantation, low‑intensity MRI signals were detected on the T2 and T2* sequences at the infarction margins. After 3 weeks following transplantation, low‑intensity signals started to appear within the infarcted area; however, the signal intensity subsequently decreased and became indistinct. Immunohistochemistry revealed that the SPIO‑labeled BMSCs migrated from the margin into the infarcted region. In conclusion, the BMSCs were readily labeled with SPIO and in vivo and MRI tracking demonstrated that the SPIO‑labeled BMSCs established and grew in the infarcted myocardium.
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View References

1 

Makino S, Fukuda K, Miyoshi S, et al: Cardiomyocytes can be generated from marrow stromal cells in vitro. J Clin Invest. 103:697–705. 1999. View Article : Google Scholar : PubMed/NCBI

2 

Anversa P, Kajstura J, Leri A and Bolli R: Life and death of cardiac stem cells: a paradigm shift in cardiac biology. Circulation. 113:1451–1463. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Feng X, He X, Li K, et al: The effects of pulsed electromagnetic fields on the induction of rat bone marrow mesenchymal stem cells to differentiate into cardiomyocytes-like cells in vitro. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 28:676–682. 2011.(In Chinese).

4 

Orlic D, Kajstura J, Chimenti S, et al: Bone marrow cells regenerate infarcted myocardium. Nature. 410:701–705. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Bulte JW and Kraitchman DL: Iron oxide MR contrast agents for molecular and cellular imaging. NMR Biomed. 17:484–499. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Ju S, Teng GJ, Lu H, et al: In vivo MR tracking of mesenchymal stem cells in rat liver after intrasplenic transplantation. Radiology. 245:206–215. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Swijnenburg RJ, van der Bogt KE, Sheikh AY, Cao F and Wu JC: Clinical hurdles for the transplantation of cardiomyocytes derived from human embryonic stem cells: role of molecular imaging. Curr Opin Biotechnol. 18:38–45. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Weissleder R: Molecular imaging: exploring the next frontier. Radiology. 212:609–614. 1999. View Article : Google Scholar : PubMed/NCBI

9 

Arbab AS, Bashaw LA, Miller BR, et al: Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging. Radiology. 229:838–846. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Bos C, Delmas Y, Desmouliere A, et al: In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver. Radiology. 233:781–789. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Bruce IJ and Sen T: Surface modification of magnetic nanoparticles with alkoxysilanes and their application in magnetic bioseparations. Langmuir. 21:7029–7035. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Geraldes CF and Laurent S: Classification and basic properties of contrast agents for magnetic resonance imaging. Contrast Media Mol Imaging. 4:1–23. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Henning TD, Saborowski O, Golovko D, et al: Cell labeling with the positive MR contrast agent Gadofluorine M. Eur Radiol. 17:1226–1234. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Kim D, Hong KS and Song J: The present status of cell tracking methods in animal models using magnetic resonance imaging technology. Mol Cells. 23:132–137. 2007.PubMed/NCBI

15 

Matuszewski L, Persigehl T, Wall A, et al: Cell tagging with clinically approved iron oxides: feasibility and effect of lipofection, particle size, and surface coating on labeling efficiency. Radiology. 235:155–161. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Shen J, Zhong XM, Duan XH, et al: Magnetic resonance imaging of mesenchymal stem cells labeled with dual (MR and fluorescence) agents in rat spinal cord injury. Acad Radiol. 16:1142–1154. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Hill JM, Dick AJ, Raman VK, et al: Serial cardiac magnetic resonance imaging of injected mesenchymal stem cells. Circulation. 108:1009–1014. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Amsalem Y, Mardor Y, Feinberg MS, et al: Iron-oxide labeling and outcome of transplanted mesenchymal stem cells in the infarcted myocardium. Circulation. 116:I38–I45. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Kraitchman DL, Tatsumi M, Gilson WD, et al: Dynamic imaging of allogeneic mesenchymal stem cells trafficking to myocardial infarction. Circulation. 112:1451–1461. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Frank JA, Miller BR, Arbab AS, et al: Clinically applicable labeling of mammalian and stem cells by combining superparamagnetic iron oxides and transfection agents. Radiology. 228:480–487. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Hansen HA and Weinfeld A: Hemosiderin estimations and sideroblast counts in the differential diagnosis of iron deficiency and other anemias. Acta Med Scand. 165:333–356. 1959. View Article : Google Scholar : PubMed/NCBI

22 

Tomita S, Li RK, Weisel RD, et al: Autologous transplantation of bone marrow cells improves damaged heart function. Circulation. 100:II247–II256. 1999. View Article : Google Scholar : PubMed/NCBI

23 

Chen SL, Fang WW, Ye F, et al: Effect on left ventricular function of intracoronary transplantation of autologous bone marrow mesenchymal stem cell in patients with acute myocardial infarction. Am J Cardiol. 94:92–95. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Lunde K, Solheim S, Aakhus S, et al: Exercise capacity and quality of life after intracoronary injection of autologous mononuclear bone marrow cells in acute myocardial infarction: results from the Autologous Stem cell Transplantation in Acute Myocardial Infarction (ASTAMI) randomized controlled trial. Am Heart J. 154(710): e711–e718. 2007.

25 

Beitnes JO, Hopp E, Lunde K, et al: Long-term results after intracoronary injection of autologous mononuclear bone marrow cells in acute myocardial infarction: the ASTAMI randomised, controlled study. Heart. 95:1983–1989. 2009. View Article : Google Scholar

26 

Beitnes JO, Gjesdal O, Lunde K, et al: Left ventricular systolic and diastolic function improve after acute myocardial infarction treated with acute percutaneous coronary intervention, but are not influenced by intracoronary injection of autologous mononuclear bone marrow cells: a 3 year serial echocardiographic sub-study of the randomized-controlled ASTAMI study. Eur J Echocardiogr. 12:98–106. 2011.

27 

Lunde K, Solheim S, Aakhus S, et al: Intracoronary injection of mononuclear bone marrow cells in acute myocardial infarction. N Engl J Med. 355:1199–1209. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Meyer GP, Wollert KC, Lotz J, et al: Intracoronary bone marrow cell transfer after myocardial infarction: eighteen months’ follow-up data from the randomized, controlled BOOST (Bone marrow transfer to enhance ST-elevation infarct regeneration) trial. Circulation. 113:1287–1294. 2006.PubMed/NCBI

29 

Paxinos G and Katritsis D: Autologous stem cell transplantation for regeneration of infarcted myocardium: clinical trials. Hellenic J Cardiol. 49:163–168. 2008.PubMed/NCBI

30 

Schächinger V, Erbs S, Elsässer A, et al: Improved clinical outcome after intracoronary administration of bone-marrow-derived progenitor cells in acute myocardial infarction: final 1-year results of the REPAIR-AMI trial. Eur Heart J. 27:2775–2783. 2006.

31 

Terrovitis J, Stuber M, Weiss RG, Leppo M, Youssef A, Gerstenblith G and Marban E: Iron-labeled stem cells seen by magnetic resonance imaging: dead or alive? Circulation. 114:2642006.

32 

Arbab AS, Yocum GT, Kalish H, et al: Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI. Blood. 104:1217–1223. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Stuckey DJ, Carr CA, Martin-Rendon E, et al: Iron particles for noninvasive monitoring of bone marrow stromal cell engraftment into, and isolation of viable engrafted donor cells from, the heart. Stem Cells. 24:1968–1975. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Terrovitis J, Stuber M, Youssef A, et al: Magnetic resonance imaging overestimates ferumoxide-labeled stem cell survival after transplantation in the heart. Circulation. 117:1555–1562. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Zhou R, Idiyatullin D, Moeller S, et al: SWIFT detection of SPIO-labeled stem cells grafted in the myocardium. Magn Reson Med. 63:1154–1161. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Nagyova M, Slovinska L, Blasko J, et al: A comparative study of PKH67, DiI, and BrdU labeling techniques for tracing rat mesenchymal stem cells. In Vitro Cell Dev Biol Anim. 50:656–663. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hua P, Wang YY, Liu LB, Liu JL, Liu JY, Yang YQ and Yang SR: In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction. Mol Med Rep 11: 113-120, 2015.
APA
Hua, P., Wang, Y., Liu, L., Liu, J., Liu, J., Yang, Y., & Yang, S. (2015). In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction. Molecular Medicine Reports, 11, 113-120. https://doi.org/10.3892/mmr.2014.2649
MLA
Hua, P., Wang, Y., Liu, L., Liu, J., Liu, J., Yang, Y., Yang, S."In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction". Molecular Medicine Reports 11.1 (2015): 113-120.
Chicago
Hua, P., Wang, Y., Liu, L., Liu, J., Liu, J., Yang, Y., Yang, S."In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction". Molecular Medicine Reports 11, no. 1 (2015): 113-120. https://doi.org/10.3892/mmr.2014.2649
Copy and paste a formatted citation
x
Spandidos Publications style
Hua P, Wang YY, Liu LB, Liu JL, Liu JY, Yang YQ and Yang SR: In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction. Mol Med Rep 11: 113-120, 2015.
APA
Hua, P., Wang, Y., Liu, L., Liu, J., Liu, J., Yang, Y., & Yang, S. (2015). In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction. Molecular Medicine Reports, 11, 113-120. https://doi.org/10.3892/mmr.2014.2649
MLA
Hua, P., Wang, Y., Liu, L., Liu, J., Liu, J., Yang, Y., Yang, S."In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction". Molecular Medicine Reports 11.1 (2015): 113-120.
Chicago
Hua, P., Wang, Y., Liu, L., Liu, J., Liu, J., Yang, Y., Yang, S."In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction". Molecular Medicine Reports 11, no. 1 (2015): 113-120. https://doi.org/10.3892/mmr.2014.2649
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