Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2015 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2015 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL‑RVG) in vitro

  • Authors:
    • Yulan Yan
    • Bing Liang
    • Jin Zhang
    • Yang Liu
    • Xuefeng Bu
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China, Department of Internal Medicine, Clinical Medicine College of Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China, Department of General Surgery, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China
  • Pages: 317-326
    |
    Published online on: October 15, 2014
       https://doi.org/10.3892/mmr.2014.2657
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Newcastle disease virus (NDV) is a member of the genus Avulavirus in the Paramyxoviridae family and its antitumor properties depend on its ability to kill malignant cells while not affecting normal cells. The present study investigated a recombinant avirulent NDV LaSota strain (wild‑type NDV strain) expressing the rabies virus glycoprotein (rL‑RVG), examined its oncolytic effect on the lung adenocarcinoma A549 cell line and evaluated its potential to serve as a vaccine against lung cancer. A549 cells were infected with the rL‑RVG virus and analyzed by MTT, western blot, polymerase chain reaction (PCR), immunofluorescence, terminal deoxynucleotidyl transferase dUTP nick end labeling and flow‑cytometric analyses. PCR, western blot and immunofluorescence showed that the RVG gene and protein were stably expressed in A549 cells following infection with rL‑RVG. The growth of A549 cells in the rL‑RVG group was inhibited more effectively compared to those infected with the wild‑type NDV strain. MTT results showed that cell growth inhibition rates in the rL‑RVG group were significantly higher than those in the NDV group (P<0.05). Early apoptosis in the rL‑RVG group was also more evident, with the apoptotic index being increased in rL‑RVG group. The expression of the pro‑apoptotic proteins caspase‑3, ‑8 and ‑9 increased. The expression of caspase‑3 decreased following application of the broad‑specificity caspase inhibitor Z‑VAD‑FMK. However, the expression of the inhibitory apoptosis protein B‑cell lymphoma 2 (bcl‑2) did not change, but bcl‑2‑associated X/bcl‑2 ratio was higher in the rL‑RVG group than that in the NDV group. The rL‑RVG strain was able to suppress lung cancer cell growth and promote lung cancer cell apoptosis to a greater extent than the wild‑type NDV strain. Therefore, the rL‑RVG strain is a potent antitumor agent.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Jemal A, Tiwari RC, Murray T, et al: American Cancer Society: Cancer statistics, 2004. CA Cancer J Clin. 54:8–29. 2004. View Article : Google Scholar

2 

DeSantis C, Naishadham D and Jemal A: Cancer statistics for African Americans, 2013. CA Cancer J Clin. 63:151–166. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Zhang J: The treatment of lung carcinoma in aged people. Chin J Geriatr. 4:316–318. 2001.

4 

Vähä-Koskela MJ, Heikkilä JE and Hinkkanen AE: Oncolytic viruses in cancer therapy. Cancer Letters. 254:178–216. 2007.

5 

Lam HY, Yeap SK, Rasoli M, et al: Safety and clinical usage of newcastle disease virus in cancer therapy. J Biomed Biotechnol. 2011:7187102011.PubMed/NCBI

6 

Yusoff K and Tan WS: Newcastle disease virus: macromolecules and opportunities. Avian Pathol. 30:439–455. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Römer-Oberdörfer A, Werner O, Veits J, Mebatsion T and Mettenleiter TC: Contribution of the length of the HN protein and the sequence of the F protein cleavage site to Newcastle disease virus pathogenicity. J Gen Virol. 84(Pt 11): 3121–3129. 2003.PubMed/NCBI

8 

Wu Y, Zhang X, Wang X, Wang L, Hu S, Liu X and Meng S: Apoptin enhances the oncolytic properties of Newcastle disease virus. Intervirology. 55:276–86. 2012.PubMed/NCBI

9 

Silverstein SC and Marcus PI: Early stages of newcastle disease virus-hela cell interaction: an electron microscopic study. Virology. 23:370–380. 1964. View Article : Google Scholar : PubMed/NCBI

10 

Johnson N, McElhinney LM, Smith J, Lowings P and Fooks AR: Phylogenetic comparison of the genus lyssavirus using distal coding sequences of the glycoprotein and nucleoprotein genes. Arch Virol. 147:2111–2123. 2002. View Article : Google Scholar

11 

Préhaud C, Lay S, Dietzschold B and Lafon M: Glycoprotein of nonpathogenic rabies viruses is a key determinant of human cell apoptosis. J Virol. 77:10537–10547. 2003.PubMed/NCBI

12 

Ge J, Wang X, Tao L, et al: Newcastle disease virus-vectored rabies vaccine is safe, highly immunogenic, and provides long-lasting protection in dogs and cats. J Virol. 85:8241–8252. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Reed LJ and Muench H: A simple method of estimating fifty percent endpoints. Am J Hyg. 27:493–497. 1938.

14 

Molouki A, Hsu YT, Jahanshiri F, Rosli R and Yusoff K: Newcastle disease virus infection promotes bax redistribution to mitochondria and cell death in HeLa cells. Intervirology. 53:87–94. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Ali R, Alabsi AM, Ali AM, et al: Cytolytic effects and apoptosis induction of Newcastle disease virus strain AF2240 on anaplastic astrocytoma brain tumor cell line. Neurochem Res. 36:2051–2062. 2011. View Article : Google Scholar

16 

Batliwalla FM, Bateman BA, Serrano D, et al: A 15-year follow-up of AJCC stage III malignant melanoma patients treated postsurgically with Newcastle diseasevirus (NDV) oncolysate and determination of alterations in the CD8 T cell repertoire. Mol Med. 4:783–794. 1998.

17 

Pecora AL, Rizvi N, Cohen GI, et al: Phase I trial of intravenous administration of PV701, an oncolytic virus, in patients with advanced solid cancers. J Clin Oncol. 20:2251–2266. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Csatary LK, Moss RW, Beuth J, Töröcsik B, Szeberenyi J and Bakacs T: Beneficial treatment of patients with advanced cancer using a Newcastle disease virus vaccine (MTH-68/H). Anticancer Res. 19:635–638. 1999.PubMed/NCBI

19 

Freeman AI, Zakay-Rones Z, Gomori JM, et al: Phase I/II trial of intravenous NDV-HUJ oncolytic virus in recurrent glioblastoma multiforme. Mol Ther. 13:221–228. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Ravindra PV, Tiwari AK, Sharma B and Chauhan RS: Newcastle disease virus as an oncolytic agent. Indian J Med Res. 130:507–513. 2009.PubMed/NCBI

21 

Li X, Jin N, Mi Z, et al: Antitumor effects of a recombinant fowlpox virus expressing apoptin in vivo and in vitro. Int J Cancer. 119:2948–2957. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Pietersen AM, van der Eb MM, Rademaker HJ, et al: Specific tumor-cell killing with adenovirus vectors containing the apoptin gene. Gene Ther. 6:882–892. 1999. View Article : Google Scholar : PubMed/NCBI

23 

Wu Y, Zhang X, Wang X, et al: Apoptin enhances the oncolytic properties of Newcastle disease virus. Intervirology. 55:276–286. 2012.PubMed/NCBI

24 

Zhao H, Janke M, Fournier P and Schirrmacher V: Recombinant Newcastle disease virus expressing human interleukin-2 serves as a potential candidate for tumor therapy. Virus Res. 136:75–80. 2008. View Article : Google Scholar

25 

Ravindra PV, Tiwari AK, Ratta B, et al: Induction of apoptosis in Vero cells by Newcastle disease virus requires viral replication, de-novo protein synthesis and caspase activation. Virus Res. 133:285–90. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Zamarin D and Palese P: Oncolytic Newcastle disease virus for cancer therapy: old challenges and new directions. Future Microbiol. 7:347–367. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Elankumaran S, Rockemann D and Samal SK: Newcastle disease virus exerts oncolysis by both intrinsic and extrinsic caspase-dependent pathways of cell death. J Virol. 80:7522–7534. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Hirsch T, Marzo I and Kroemer G: Role of the mitochondrial permeability transition pore in apoptosis. Biosci Rep. 17:67–76. 1997. View Article : Google Scholar : PubMed/NCBI

29 

Plummer HK III, Dhar M and Schuller HM: Expression of the alpha7 nicotinic acetylcholine receptor in human lung cells. Respir Res. 6:292005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yan Y, Liang B, Zhang J, Liu Y and Bu X: Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL‑RVG) in vitro. Mol Med Rep 11: 317-326, 2015.
APA
Yan, Y., Liang, B., Zhang, J., Liu, Y., & Bu, X. (2015). Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL‑RVG) in vitro. Molecular Medicine Reports, 11, 317-326. https://doi.org/10.3892/mmr.2014.2657
MLA
Yan, Y., Liang, B., Zhang, J., Liu, Y., Bu, X."Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL‑RVG) in vitro". Molecular Medicine Reports 11.1 (2015): 317-326.
Chicago
Yan, Y., Liang, B., Zhang, J., Liu, Y., Bu, X."Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL‑RVG) in vitro". Molecular Medicine Reports 11, no. 1 (2015): 317-326. https://doi.org/10.3892/mmr.2014.2657
Copy and paste a formatted citation
x
Spandidos Publications style
Yan Y, Liang B, Zhang J, Liu Y and Bu X: Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL‑RVG) in vitro. Mol Med Rep 11: 317-326, 2015.
APA
Yan, Y., Liang, B., Zhang, J., Liu, Y., & Bu, X. (2015). Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL‑RVG) in vitro. Molecular Medicine Reports, 11, 317-326. https://doi.org/10.3892/mmr.2014.2657
MLA
Yan, Y., Liang, B., Zhang, J., Liu, Y., Bu, X."Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL‑RVG) in vitro". Molecular Medicine Reports 11.1 (2015): 317-326.
Chicago
Yan, Y., Liang, B., Zhang, J., Liu, Y., Bu, X."Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL‑RVG) in vitro". Molecular Medicine Reports 11, no. 1 (2015): 317-326. https://doi.org/10.3892/mmr.2014.2657
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team