Identification of anesthetic‑induced expression changes using DNA microarray

Yang,Zaiqi; Zhang,Mengyuan; Wang,Gongming; Wei,Pihong; Gao,Shenqiang

doi:10.3892/mmr.2014.2669

Identification of anesthetic‑induced expression changes using DNA microarray

Authors:
- Zaiqi Yang
- Mengyuan Zhang
- Gongming Wang
- Pihong Wei
- Shenqiang Gao
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Affiliations: Department of Anesthesiology, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Anesthesiology, Taian Central Hospital, Taian, Shandong 271000, P.R. China
Published online on: October 16, 2014 https://doi.org/10.3892/mmr.2014.2669
Pages: 589-596

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Abstract

The present study aimed to identify changes in atrial gene expression induced by sevoflurane and propofol using DNA microarray. The expression profiles of GSE4386 in atrial samples, obtained from patients who had received either the anesthetic gas sevoflurane or the intravenous anesthetic propofol prior to and following off‑pump coronary artery bypass graft (CABG) surgery, were downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) in the sevoflurane and the propofol groups were then identified and compared. Subsequently, a functional enrichment analysis was performed for the DEGs. The interactive functional modules for common, sevoflurane‑specific and propofol‑specific DEGs were then constructed for analysis of the biological processes. The percentages of common DEGs were 31.3 (275/879) and 94.8% (275/290) in the sevoflurane group and propofol groups, respectively. The functional categories for the common, sevoflurane‑specific and propofol‑specific DEGs were similar. Overall, two, one, and one functional modules were identified for the common DEGs, propofol specific DEGs and sevoflurane specific DEGs, respectively. DEGs in the modules were involved in cellular processes, including the ʻregulation of transcriptionʼ and ʻregulation of cellular processʼ, which were similar to the functional annotations for the DEGs. Therefore, sevoflurane and propofol may synergistically reduce myocardial reperfusion injury in patients undergoing off‑pump CABG surgery.

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1	Shekar PS: Cardiology patient page. On-pump and off-pump coronary artery bypass grafting. Circulation. 113:e51–e52. 2006. View Article : Google Scholar
2	Ehsan A, Shekar P and Aranki S: Innovative surgical strategies: Minimally invasive CABG and off-pump CABG. Curr Treat Options Cardiovasc Med. 6:43–51. 2004. View Article : Google Scholar : PubMed/NCBI
3	Palmerini T, Biondi-Zoccai G, Riva DD, et al: Risk of stroke with percutaneous coronary intervention compared with on-pump and off-pump coronary artery bypass graft surgery: Evidence from a comprehensive network meta-analysis. Am Heart J. 165:910–917. 2013. View Article : Google Scholar
4	Harskamp RE, Lopes RD, Baisden CE, De Winter RJ and Alexander JH: Saphenous vein graft failure after coronary artery bypass surgery: pathophysiology, management and future directions. Ann Surg. 257:824–833. 2013. View Article : Google Scholar : PubMed/NCBI
5	Bakaeen FG, Chu D, Kelly RF, et al: Performing Coronary Artery Bypass Grafting Off-Pump May Compromise Long-Term Survival in a Veteran Population. Ann Thorac Surg. 95:1952–1958. 2013. View Article : Google Scholar : PubMed/NCBI
6	Moller CH, Penninga L, Wetterslev J, Steinbruchel DA and Gluud C: Off-pump versus on-pump coronary artery bypass grafting for ischaemic heart disease. Cochrane Database Syst Rev. 3:CD0072242012.PubMed/NCBI
7	Nathoe HM, Van Dijk D, Jansen EW, et al: A comparison of on-pump and off-pump coronary bypass surgery in low-risk patients. N Engl J Med. 348:394–402. 2003. View Article : Google Scholar : PubMed/NCBI
8	Orhan G, Sargin M, Senay S, et al: Systemic and myocardial inflammation in traditional and off-pump cardiac surgery. Tex Heart Inst J. 34:160–165. 2007.PubMed/NCBI
9	Selvanayagam JB, Petersen SE, Francis JM, et al: Effects of off-pump versus on-pump coronary surgery on reversible and irreversible myocardial injury: a randomized trial using cardiovascular magnetic resonanceimaging and biochemical markers. Circulation. 109:345–350. 2004. View Article : Google Scholar
10	Menasché P: The systemic factor: the comparative roles of cardiopulmonary bypass and off-pump surgery in the genesis of patient injury during and following cardiac surgery. Ann Thorac Surg. 72:S2260–S2265; discussion S2265–S2266, S2267–S2270. 2001.PubMed/NCBI
11	Tomic V, Russwurm S, Moller E, et al: Transcriptomic and proteomic patterns of systemic inflammation in on-pump and off-pump coronary artery bypass grafting. Circulation. 112:2912–2920. 2005.
12	Brown J, Hernandez F, Beaulieu P, Clough R, Whited C, et al: Off-pump coronary artery bypass does not influence biomarkers of brain injury, but does exacerbate the systemic inflammatory response. J Clin Cell Immunik. S2:0012011.
13	Frassdorf J, De Hert S and Schlack W: Anaesthesia and myocardial ischaemia/reperfusion injury. Br J Anaesth. 103:89–98. 2009. View Article : Google Scholar : PubMed/NCBI
14	Varadarajan SG, An J, Novalija E and Stowe DF: Sevoflurane before or after ischemia improves contractile and metabolic function while reducing myoplasmic Ca(²⁺) loading in intact hearts. Anesthesiology. 96:125–133. 2002. View Article : Google Scholar : PubMed/NCBI
15	Preckel B, Schlack W, Comfère T, Obal D, Barthel H and Thämer V: Effects of enflurane, isoflurane, sevoflurane and desflurane on reperfusion injury after regional myocardial ischaemia in the rabbit heart in vivo. Br J Anaesth. 81:905–912. 1998. View Article : Google Scholar
16	Lin E and Symons JA: Volatile anaesthetic myocardial protection: a review of the current literature. HSR Proc Intensive Care Cardiovasc Anesth. 2:105–109. 2010.
17	Garcia C, Julier K, Bestmann L, et al: Preconditioning with sevoflurane decreases PECAM-1 expression and improves one-year cardiovascular outcome in coronary artery bypass graft surgery. Br J Anaesth. 94:159–165. 2005.PubMed/NCBI
18	Kawamura T, Kadosaki M, Nara N, et al: Effects of sevoflurane on cytokine balance in patients undergoing coronary artery bypass graft surgery. J Cardiothorac Vasc Anesth. 20:503–508. 2006. View Article : Google Scholar : PubMed/NCBI
19	Huang Z, Zhong X, Irwin MG, et al: Synergy of isoflurane preconditioning and propofol postconditioning reduces myocardial reperfusion injury in patients. Clin Sci (Lond). 121:57–69. 2011. View Article : Google Scholar
20	Corcoran TB, Engel A, Sakamoto H, O’shea A, O’callaghan-Enright S and Shorten GD: The effects of propofol on neutrophil function, lipid peroxidation and inflammatory response during elective coronary artery bypass grafting in patients with impaired ventricular function. Br J Anaesth. 97:825–831. 2006. View Article : Google Scholar
21	Sun HY, Xue FS, Xu YC, et al: Propofol improves cardiac functional recovery after ischemia-reperfusion by upregulating nitric oxide synthase activity in the isolated rat hearts. Chin Med J (Engl). 122:3048–3054. 2009.
22	Wang HY, Wang GL, Yu YH and Wang Y: The role of phosphoinositide-3-kinase/Akt pathway in propofol-induced postconditioning against focal cerebral ischemia-reperfusion injury in rats. Brain Res. 1297:177–184. 2009. View Article : Google Scholar
23	Kottenberg E, Thielmann M, Bergmann L, et al: Protection by remote ischemic preconditioning during coronary artery bypass graft surgery with isoflurane but not propofol - a clinical trial. Acta Anaesthesiol Scand. 56:30–38. 2012. View Article : Google Scholar
24	Zaugg M, Lucchinetti E, Spahn DR, Pasch T, Garcia C and Schaub MC: Differential effects of anesthetics on mitochondrial K(ATP) channel activity and cardiomyocyte protection. Anesthesiology. 97:15–23. 2002. View Article : Google Scholar : PubMed/NCBI
25	Müllenheim J, Ebel D, Frässdorf J, Preckel B, Thämer V and Schlack W: Isoflurane preconditions myocardium against infarction via release of free radicals. Anesthesiology. 96:934–940. 2002.PubMed/NCBI
26	Heusch G, Boengler K and Schulz R: Cardioprotection: nitric oxide, protein kinases and mitochondria. Circulation. 118:1915–1919. 2008. View Article : Google Scholar : PubMed/NCBI
27	He W, Zhang FJ, Wang SP, Chen G, Chen CC and Yan M: Postconditioning of sevoflurane and propofol is associated with mitochondrial permeability transition pore. J Zhejiang Univ Sci B. 9:100–108. 2008. View Article : Google Scholar : PubMed/NCBI
28	Lucchinetti E, Hofer C, Bestmann L, et al: Gene regulatory control of myocardial energy metabolism predicts postoperative cardiac function in patients undergoing off-pump coronary artery bypass graft surgery: inhalational versus intravenous anesthetics. Anesthesiology. 106:444–457. 2007. View Article : Google Scholar
29	Fujita A, Sato JR, de Rodrigues LO, Ferreira CE and Sogayar MC: Evaluating different methods of microarray data normalization. BMC Bioinformatic. 7:4692006. View Article : Google Scholar : PubMed/NCBI
30	Smyth GK: Limma: linear models for microarray data. Bioinformatics and Computational Biology Solutions using R and Bioconductor. Gentleman R, Carey V, Dudoit S, Irazarry R and Huber W: Springer; New York, NY: pp. 397–420. 2005, View Article : Google Scholar
31	Benjamini Y and Hochberg Y: Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc B. 1:289–300. 1995.
32	Huang Da W, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID Bioinformatics Resources. Nature Protoc. 4:44–57. 2009.PubMed/NCBI
33	Tatusov RL, Natale DA, Garkavtsev IV, et al: The COG database: new developments in phylogenetic classification of proteins from complete genomes. Nucleic Acids Res. 29:22–28. 2001. View Article : Google Scholar : PubMed/NCBI
34	Altschul SF, Gish W, Miller W, Myers EW and Lipman DJ: Basic local alignment search tool. J Mol Biol. 215:403–410. 1990. View Article : Google Scholar
35	Zhang B, Kirov S and Snoddy J: WebGestalt: an integrated system for exploring gene sets in various biological contexts. Nucleic Acids Res. 33:W741–W748. 2005. View Article : Google Scholar : PubMed/NCBI
36	Shi G, Zhang L and Jiang T: MSOAR 2.0: Incorporating tandem duplications into ortholog assignment based on genome rearrangement. BMC Bioinformatics. 11:102010. View Article : Google Scholar : PubMed/NCBI
37	Malik V, Kale SC, Chowdhury UK, Ramakrishnan L, Chauhan S and Kiran U: Myocardial injury in coronary artery bypass grafting: On-pump versus off-pump comparison by measuring heart-type fatty-acid-binding protein release. Tex Heart Inst J. 33:321–327. 2006.
38	Mathur S, Farhangkhgoee P and Karmazyn M: Cardioprotective effects of propofol and sevoflurane in ischemic and reperfused rat hearts: role of K(ATP) channels and interaction with the sodium-hydrogen exchange inhibitor HOE 642 (cariporide). Anesthesiology. 91:1349–1360. 1999. View Article : Google Scholar : PubMed/NCBI
39	Nobori K, Ito H, Tamamori-Adachi M, et al: ATF3 inhibits doxorubicin-induced apoptosis in cardiac myocytes: a novel cardioprotective role of ATF3. J Mol Cell Cardiol. 34:1387–1397. 2002. View Article : Google Scholar : PubMed/NCBI
40	Seijffers R, Allchorne AJ and Woolf CJ: The transcription factor ATF-3 promotes neurite outgrowth. Mol Cell Neurosci. 32:143–154. 2006. View Article : Google Scholar : PubMed/NCBI
41	Francis JS, Dragunow M and During MJ: Over expression of ATF-3 protects rat hippocampal neurons from in vivo injection of kainic acid. Brain Res Mol Brain Res. 124:199–203. 2004. View Article : Google Scholar : PubMed/NCBI
42	Chu HM, Tan Y, Kobierski LA, Balsam LB and Comb MJ: Activating transcription factor-3 stimulates 3′,5′-cyclic adenosine monophosphate-dependent gene expression. Mol Endocrinol. 8:59–68. 1994.
43	Weitzman JB, Fiette L, Matsuo K and Yaniv M: JunD protects cells from p53-dependent senescence and apoptosis. Mol Cell. 6:1109–1119. 2000. View Article : Google Scholar : PubMed/NCBI
44	Bergman MR, Cheng S, Honbo N, Piacentini L, Karliner JS and Lovett DH: A functional activating protein 1 (AP-1) site regulates matrix metalloproteinase 2 (MMP-2) transcription by cardiac cells through interactions with JunB-Fra1 and JunB-FosB heterodimers. Biochem J. 369:485–496. 2003. View Article : Google Scholar
45	Alfonso-Jaume MA, Bergman MR, Mahimkar R, et al: Cardiac ischemia-reperfusion injury induces matrix metalloproteinase-2 expression through the AP-1 components FosB and JunB. Am J Physiol Heart Circ Physiol. 291:H1838–H1846. 2006. View Article : Google Scholar
46	Kumar S, Boehm J and Lee JC: p38 MAP kinases: key signalling molecules as therapeutic targets for inflammatory diseases. Nat Rev Drug Discov. 2:717–726. 2003. View Article : Google Scholar : PubMed/NCBI
47	Muslin AJ: MAPK signalling in cardiovascular health and disease: molecular mechanisms and therapeutic targets. Clin Sci (Lond). 115:203–218. 2008. View Article : Google Scholar : PubMed/NCBI
48	Wang Y, Huang S, Sah VP, et al: Cardiac muscle cell hypertrophy and apoptosis induced by distinct members of the p38 mitogen-activated protein kinase family. J Biol Chem. 273:2161–2168. 1998. View Article : Google Scholar : PubMed/NCBI
49	Boonyasrisawat W, Eberle D, Bacci S, et al: Tag polymorphisms at the A20 (TNFAIP3) locus are associated with lower gene expression and increased risk of coronary artery disease in type 2 diabetes. Diabetes. 56:499–505. 2007. View Article : Google Scholar : PubMed/NCBI
50	Xue H, Wang SX, Wang XJ, et al: Variants of tumor necrosis factor-induced protein 3 gene are associated with left ventricular hypertrophy in hypertensive patients. Chin Med J (Engl). 124:1498–1503. 2011.
51	De Keulenaer GW, Wang Y, Feng Y, et al: Identification of IEX-1 as a biomechanically controlled nuclear factor-kappaB target gene that inhibits cardiomyocyte hypertrophy. Circ Res. 90:690–696. 2002.
52	Hirotani S, Otsu K, Nishida K, et al: Involvement of nuclear factor-kappaB and apoptosis signal-regulating kinase 1 in G-protein-coupled receptor agonist-induced cardiomyocyte hypertrophy. Circulation. 105:509–515. 2002. View Article : Google Scholar