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Article

Transforming growth factor‑β1‑induced epithelial to mesenchymal transition increases mitochondrial content in the A549 non‑small cell lung cancer cell line

  • Authors:
    • Yunhua Xu
    • Shun Lu
  • View Affiliations / Copyright

    Affiliations: Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, P.R. China
  • Pages: 417-421
    |
    Published online on: October 16, 2014
       https://doi.org/10.3892/mmr.2014.2678
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Abstract

The mitochondrial genome DNA copy number is critical for the functional maintenance of the mitochondria and energy acquisition for cell metabolism. Epithelial to mesenchymal transition (EMT) is an important process during embryonic development and has also been hypothesized to exhibit a significant role in cancer cell invasion and metastasis. In the present study, EMT was induced in the A549 non‑small cell lung cancer (NSCLC) cell line, using transforming growth factor‑β1 (TGF‑β1) and changes in mitochondrial content, mitochondrial DNA (mtDNA) copy number and protein cytochrome c (Cyt c) were determined by reverse transcritpion‑quantitative polymerase chain reaction and western blot analysis, respectively. mtDNA copy number and Cyt c protein levels were observed to increase following the induction of EMT in NSCLC cells. Results of the current study indicate that energy metabolism is adapted to facilitate EMT in NSCLC cells.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Y and Lu S: Transforming growth factor‑β1‑induced epithelial to mesenchymal transition increases mitochondrial content in the A549 non‑small cell lung cancer cell line. Mol Med Rep 11: 417-421, 2015.
APA
Xu, Y., & Lu, S. (2015). Transforming growth factor‑β1‑induced epithelial to mesenchymal transition increases mitochondrial content in the A549 non‑small cell lung cancer cell line. Molecular Medicine Reports, 11, 417-421. https://doi.org/10.3892/mmr.2014.2678
MLA
Xu, Y., Lu, S."Transforming growth factor‑β1‑induced epithelial to mesenchymal transition increases mitochondrial content in the A549 non‑small cell lung cancer cell line". Molecular Medicine Reports 11.1 (2015): 417-421.
Chicago
Xu, Y., Lu, S."Transforming growth factor‑β1‑induced epithelial to mesenchymal transition increases mitochondrial content in the A549 non‑small cell lung cancer cell line". Molecular Medicine Reports 11, no. 1 (2015): 417-421. https://doi.org/10.3892/mmr.2014.2678
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y and Lu S: Transforming growth factor‑β1‑induced epithelial to mesenchymal transition increases mitochondrial content in the A549 non‑small cell lung cancer cell line. Mol Med Rep 11: 417-421, 2015.
APA
Xu, Y., & Lu, S. (2015). Transforming growth factor‑β1‑induced epithelial to mesenchymal transition increases mitochondrial content in the A549 non‑small cell lung cancer cell line. Molecular Medicine Reports, 11, 417-421. https://doi.org/10.3892/mmr.2014.2678
MLA
Xu, Y., Lu, S."Transforming growth factor‑β1‑induced epithelial to mesenchymal transition increases mitochondrial content in the A549 non‑small cell lung cancer cell line". Molecular Medicine Reports 11.1 (2015): 417-421.
Chicago
Xu, Y., Lu, S."Transforming growth factor‑β1‑induced epithelial to mesenchymal transition increases mitochondrial content in the A549 non‑small cell lung cancer cell line". Molecular Medicine Reports 11, no. 1 (2015): 417-421. https://doi.org/10.3892/mmr.2014.2678
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