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Proteasome inhibitor lactacystin enhances cisplatin cytotoxicity by increasing endoplasmic reticulum stress‑associated apoptosis in HeLa cells

  • Authors:
    • Ye Xu
    • Di Li
    • Linchuan Zeng
    • Chunyan Wang
    • Lili Zhang
    • Yan Wang
    • Yang Yu
    • Shibing Liu
    • Zhixin Li
  • View Affiliations / Copyright

    Affiliations: Medical Research Laboratory, Jilin Medical College, Jilin, Jilin 132013, P.R. China, Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Histology and Embryology, Jilin Medical College, Jilin, Jilin 132013, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 189-195
    |
    Published online on: October 16, 2014
       https://doi.org/10.3892/mmr.2014.2683
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Abstract

Cisplatin is commonly used as a therapeutic agent, despite its known adverse side effects and the occurrence of drug resistance. The development of novel methods for combination therapy with cisplatin is required in order to circumvent these limitations of cisplatin alone. The proteasome inhibitor lactacystin (LAC) has been indicated to produce anti‑tumor effects, and has previously been used as an antitumor agent in cancer treatment research; however, its effects in combination with cisplatin treatment are unknown. In the current study, the effects of LAC in combination with cisplatin treatment were investigated in HeLa human cervical cancer (HCC) cells. The results demonstrated that cisplatin treatment inhibited cell growth and induced cell apoptosis. HeLa cell exposure to cisplatin induced endoplasmic reticulum (ER) stress‑associated apoptosis, and LAC treatment increased levels of cell apoptosis and the activation of caspase‑3. Specifically, LAC treatment increased the cisplatin‑induced expression of PDI, GRP78, CHOP, cleaved caspase‑4 and cleaved caspase‑3. Together, these data indicate that LAC is able to enhance cisplatin cytotoxicity by increasing ER stress‑associated apoptosis in HeLa cells.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Y, Li D, Zeng L, Wang C, Zhang L, Wang Y, Yu Y, Liu S and Li Z: Proteasome inhibitor lactacystin enhances cisplatin cytotoxicity by increasing endoplasmic reticulum stress‑associated apoptosis in HeLa cells. Mol Med Rep 11: 189-195, 2015.
APA
Xu, Y., Li, D., Zeng, L., Wang, C., Zhang, L., Wang, Y. ... Li, Z. (2015). Proteasome inhibitor lactacystin enhances cisplatin cytotoxicity by increasing endoplasmic reticulum stress‑associated apoptosis in HeLa cells. Molecular Medicine Reports, 11, 189-195. https://doi.org/10.3892/mmr.2014.2683
MLA
Xu, Y., Li, D., Zeng, L., Wang, C., Zhang, L., Wang, Y., Yu, Y., Liu, S., Li, Z."Proteasome inhibitor lactacystin enhances cisplatin cytotoxicity by increasing endoplasmic reticulum stress‑associated apoptosis in HeLa cells". Molecular Medicine Reports 11.1 (2015): 189-195.
Chicago
Xu, Y., Li, D., Zeng, L., Wang, C., Zhang, L., Wang, Y., Yu, Y., Liu, S., Li, Z."Proteasome inhibitor lactacystin enhances cisplatin cytotoxicity by increasing endoplasmic reticulum stress‑associated apoptosis in HeLa cells". Molecular Medicine Reports 11, no. 1 (2015): 189-195. https://doi.org/10.3892/mmr.2014.2683
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y, Li D, Zeng L, Wang C, Zhang L, Wang Y, Yu Y, Liu S and Li Z: Proteasome inhibitor lactacystin enhances cisplatin cytotoxicity by increasing endoplasmic reticulum stress‑associated apoptosis in HeLa cells. Mol Med Rep 11: 189-195, 2015.
APA
Xu, Y., Li, D., Zeng, L., Wang, C., Zhang, L., Wang, Y. ... Li, Z. (2015). Proteasome inhibitor lactacystin enhances cisplatin cytotoxicity by increasing endoplasmic reticulum stress‑associated apoptosis in HeLa cells. Molecular Medicine Reports, 11, 189-195. https://doi.org/10.3892/mmr.2014.2683
MLA
Xu, Y., Li, D., Zeng, L., Wang, C., Zhang, L., Wang, Y., Yu, Y., Liu, S., Li, Z."Proteasome inhibitor lactacystin enhances cisplatin cytotoxicity by increasing endoplasmic reticulum stress‑associated apoptosis in HeLa cells". Molecular Medicine Reports 11.1 (2015): 189-195.
Chicago
Xu, Y., Li, D., Zeng, L., Wang, C., Zhang, L., Wang, Y., Yu, Y., Liu, S., Li, Z."Proteasome inhibitor lactacystin enhances cisplatin cytotoxicity by increasing endoplasmic reticulum stress‑associated apoptosis in HeLa cells". Molecular Medicine Reports 11, no. 1 (2015): 189-195. https://doi.org/10.3892/mmr.2014.2683
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