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Article

Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice

  • Authors:
    • Soo‑Min Cho
    • Jin‑A Park
    • Na‑Hyun Kim
    • Dong‑Soon Kim
    • Dan Zhang
    • Hee Yi
    • Hee‑Jung Cho
    • Ja Kyung Kim
    • Dong Ki Lee
    • Jin‑Suk Kim
    • Ho‑Chul Shin
  • View Affiliations / Copyright

    Affiliations: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143‑701, Republic of Korea, Department of Internal Medicine, Yonsei University College of Medicine, Seoul 120‑752, Republic of Korea
  • Pages: 362-366
    |
    Published online on: October 17, 2014
       https://doi.org/10.3892/mmr.2014.2687
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Abstract

The present study investigated the preventive effect of ω‑3 fatty acids against cholesterol gallstone (CG) formation. CG formation was induced in C57BL/6J mice using a lithogenic diet (LD). The mice were divided into four treatment groups: i) LD, ii) LD plus eicosapentaenoic acid (EPA), iii) LD plus docosahexaenoic acid (DHA) and iv) LD plus EPA plus DHA. Subsequent to feeding the mice the LD for four weeks, EPA and/or DHA (70 mg/kg/day) were orally administered for eight weeks. The mice in the EPA treatment groups exhibited significantly less gallstone formation than those in the LD group. By contrast, DHA treatment only slightly suppressed gallstone formation. The expression of mucin 2, 5AC, 5B and 6 was significantly decreased in the gallbladders of mice in the EPA groups (70‑90%) and the LD plus DHA group (30‑50%), compared with that in the mice in the LD group. In addition, the mRNA expression of 3‑hydroxy‑3‑methylglutaryl‑coenzyme A reductase was significantly decreased in the livers of mice in the EPA treatment group compared with that in the livers of mice in the LD group. In conclusion, EPA was found to have a dominant anti‑lithogenic effect in C57BL/6J mice.
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1 

Drevon CA: Marine oils and their effects. Nutr Rev. 50:38–45. 1992. View Article : Google Scholar

2 

Song C and Zhao S: Omega-3 fatty acid eicosapentaenoic acid. A new treatment for psychiatric and neurodegenerative diseases: a review of clinical investigations. Expert Opin Investig Drugs. 16:1627–1638. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Yokoyama M and Origasa H; JELIS Investigators. Effects of eicosapentaenoic acid on cardiovascular events in Japanese patients with hypercholesterolemia: rationale, design, and baseline characteristics of the Japan EPA Lipid Intervention Study (JELIS). Am Heart J. 146:613–620. 2003. View Article : Google Scholar

4 

Miyajima T, Tsujino T, Saito K and Yokoyama M: Effects of eicosapentaenoic acid on blood pressure, cell membrane fatty acids, and intracellular sodium concentration in essential hypertension. Hypertens Res. 24:537–542. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Peet M, Brind J, Ramchand CN, Shah S and Vankar GK: Two double-blind placebo-controlled pilot studies of eicosapentaenoic acid in the treatment of schizophrenia. Schizophr Res. 49:243–251. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Calder PC: Session 3: Joint Nutrition Society and Irish Nutrition and Dietetic Institute Symposium on ‘Nutrition and autoimmune disease’ PUFA, inflammatory processes and rheumatoid arthritis. Proc Nutr Soc. 67:409–418. 2008.

7 

Singh M: Essential fatty acids, DHA and human brain. Indian J Pediatr. 72:239–242. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Pifferi F, Perret M, Guesnet P, Aujard F and Alessandri JM: Fatty acid composition of the brain, retina, liver and adipose tissue of the grey mouse lemur (Microcebus murinus, primate). Lipids. 47:793–801. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Cunnane SC, Plourde M, Pifferi F, Bégin M, Féart C and Barberger-Gateau P: Fish, docosahexaenoic acid and Alzheimer’s disease. Prog Lipid Res. 48:239–256. 2009.

10 

Fotuhi M, Mohassel P and Yaffe K: Fish consumption, long-chain omega-3 fatty acids and risk of cognitive decline or Alzheimer disease: a complex association. Nat Clin Pract Neurol. 5:140–152. 2009. View Article : Google Scholar

11 

McNamara RK, Hahn CG, Jandacek R, Rider T, Tso P, Stanford KE and Richtand NM: Selective deficits in the omega-3 fatty acid docosahexaenoic acid in the postmortem orbitofrontal cortex of patients with major depressive disorder. Biol Psychiatry. 62:17–24. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Lee SP, LaMont JT and Carey MC: Role of gallbladder mucus hypersecretion in the evolution of cholesterol gallstones. J Clin Invest. 67:1712–1723. 1981. View Article : Google Scholar : PubMed/NCBI

13 

Wang DQ, Paigen B and Carey MC: Phenotypic characterization of Lith genes that determine susceptibility to cholesterol cholelithiasis in inbred mice: physical-chemistry of gallbladder bile. J Lipid Res. 38:1395–1411. 1997.

14 

Vilkin A, Nudelman I, Morgenstern S, Geller A, Bar Dayan Y, Levi Z, Rodionov G, Hardy B, Konikoff F, Gobbic D and Niv Y: Gallbladder inflammation is associated with increase in mucin expression and pigmented stone formation. Dig Dis Sci. 52:1613–1620. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Chuang SC, Hsi E and Lee KT: Mucin genes in gallstone disease. Clin Chim Acta. 413:1466–1471. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Lee KT and Liu TS: Mucin gene expression in gallbladder epithelium. J Formos Med Assoc. 101:762–768. 2002.PubMed/NCBI

17 

Fischer S, Müller I, Zündt BZ, Jüngst C, Meyer G and Jüngst D: Ursodeoxycholic acid decreases viscosity and sedimentable fractions of gallbladder bile in patients with cholesterol gallstones. Eur J Gastroenterol Hepatol. 16:305–311. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Venneman NG, Besselink MG, Keulemans YC, Vanberge-Henegouwen GP, Boermeester MA, Broeders IA, Go PM and van Erpecum KJ: Ursodeoxycholic acid exerts no beneficial effect in patients with symptomatic gallstones awaiting cholecystectomy. Hepatology. 43:1276–1283. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Lioudaki E, Ganotakis ES and Mikhailidis DP: Lipid lowering drugs and gallstones: a therapeutic option? Curr Pharm Des. 17:3622–3631. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Kim JK, Cho SM, Kang SH, Kim E, Yi H, Yun ES, Lee DG, Cho HJ, Paik YH, Choi YK, Haam SJ, Shin HC and Lee DK: N-3 polyunsaturated fatty acid attenuates cholesterol gallstones by suppressing mucin production with a high cholesterol diet in mice. J Gastroenterol Hepatol. 27:1745–1751. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Krauss RM, Eckel RH, Howard B, Appel LJ, Daniels SR, Deckelbaum RJ, Erdman JW Jr, Kris-Etherton P, Goldberg IJ, Kotchen TA, Lichtenstein AH, Mitch WE, Mullis R, Robinson K, Wylie-Rosett J, St Jeor S, Suttie J, Tribble DL and Bazzarre TL: AHA Dietary Guidelines: revision 2000: A statement for healthcare professionals from the Nutrition Committee of the American Heart Association. Circulation. 102:2284–2299. 2000. View Article : Google Scholar

22 

Kris-Etherton PM, Harris WS and Appel LJ; American Heart Association. Nutrition Committee. Fish consumption, fish oil, omega-3 fatty acids, and cardiovascular disease. Circulation. 106:2747–2757. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Magnuson TH, Lillemoe KD, High RC and Pitt HA: Dietary fish oil inhibits cholesterol monohydrate crystal nucleation and gallstone formation in the prairie dog. Surgery. 118:517–523. 1995. View Article : Google Scholar : PubMed/NCBI

24 

Scobey MW, Johnson FL, Parks JS and Rudel LL: Dietary fish oil effects on biliary lipid secretion and cholesterol gallstone formation in the African green monkey. Hepatology. 14:679–684. 1991.PubMed/NCBI

25 

Mizuguchi K, Yano T, Kawano H, Abei M and Tanaka N: Preventive effects of eicosapentaenoic acid (EPA) on cholesterol gallstone formation in hamsters. Nihon Yakurigaku Zasshi. 110(Suppl 1): 50P–55P. 1997.(In Japanese).

26 

Womack NA: The development of gallstones. Surg Gynecol Obstet. 133:937–945. 1971.

27 

Janowitz P, Swobodnik W, Wechsler JG, Janowitz A, Saal D and Ditschuneit H: Fish oil, enriched with polyunsaturated fatty acids of the omega-3-type accelerates the nucleation time in healthy subjects. Klin Wochenschr. 69:289–293. 1991. View Article : Google Scholar : PubMed/NCBI

28 

Weber LW, Boll M and Stampfl A: Maintaining cholesterol homeostasis: sterol regulatory element-binding proteins. World J Gastroenterol. 10:3081–3087. 2004.PubMed/NCBI

29 

Trapani L, Segatto M, Simeoni V, Balducci V, Dhawan A, Parmar VS, Prasad AK, Saso L, Incerpi S and Pallottini V: Short- and long-term regulation of 3-hydroxy 3-methylglutaryl coenzyme A reductase by a 4-methylcoumarin. Biochimie. 93:1165–1171. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cho SM, Park JA, Kim NH, Kim DS, Zhang D, Yi H, Cho HJ, Kim JK, Lee DK, Kim JS, Kim JS, et al: Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice. Mol Med Rep 11: 362-366, 2015.
APA
Cho, S., Park, J., Kim, N., Kim, D., Zhang, D., Yi, H. ... Shin, H. (2015). Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice. Molecular Medicine Reports, 11, 362-366. https://doi.org/10.3892/mmr.2014.2687
MLA
Cho, S., Park, J., Kim, N., Kim, D., Zhang, D., Yi, H., Cho, H., Kim, J. K., Lee, D. K., Kim, J., Shin, H."Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice". Molecular Medicine Reports 11.1 (2015): 362-366.
Chicago
Cho, S., Park, J., Kim, N., Kim, D., Zhang, D., Yi, H., Cho, H., Kim, J. K., Lee, D. K., Kim, J., Shin, H."Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice". Molecular Medicine Reports 11, no. 1 (2015): 362-366. https://doi.org/10.3892/mmr.2014.2687
Copy and paste a formatted citation
x
Spandidos Publications style
Cho SM, Park JA, Kim NH, Kim DS, Zhang D, Yi H, Cho HJ, Kim JK, Lee DK, Kim JS, Kim JS, et al: Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice. Mol Med Rep 11: 362-366, 2015.
APA
Cho, S., Park, J., Kim, N., Kim, D., Zhang, D., Yi, H. ... Shin, H. (2015). Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice. Molecular Medicine Reports, 11, 362-366. https://doi.org/10.3892/mmr.2014.2687
MLA
Cho, S., Park, J., Kim, N., Kim, D., Zhang, D., Yi, H., Cho, H., Kim, J. K., Lee, D. K., Kim, J., Shin, H."Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice". Molecular Medicine Reports 11.1 (2015): 362-366.
Chicago
Cho, S., Park, J., Kim, N., Kim, D., Zhang, D., Yi, H., Cho, H., Kim, J. K., Lee, D. K., Kim, J., Shin, H."Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice". Molecular Medicine Reports 11, no. 1 (2015): 362-366. https://doi.org/10.3892/mmr.2014.2687
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