Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2015 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2015 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Protein‑protein interaction network and mechanism analysis in ischemic stroke

  • Authors:
    • Zhe Quan
    • Yuan Quan
    • Bo Wei
    • Dening Fang
    • Weidong Yu
    • Hao Jia
    • Wei Quan
    • Yuguang Liu
    • Qihong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan, Shandong 250000, P.R. China, Norman Bethune Medical School of Jilin University, Changchun, Jilin 130000, P.R. China, Department of Neurosurgery, The Third Affiliated Hospital (China‑Japan Union Hospital) of Jilin University, Changchun, Jilin 130031, P.R. China, Department of Gastroenterology, Shanghai No. 8 Hospital, Shanghai 200235, P.R. China, Department of Emergency Medicine, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110000, P.R. China, Department of Infection, HuiNanXian Hospital, Huinan, Jilin 135100, P.R. China, Department of Neurosurgery, Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China
    Copyright: © Quan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 29-36
    |
    Published online on: October 17, 2014
       https://doi.org/10.3892/mmr.2014.2696
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Ischemic stroke is a leading cause of mortality and permanent disability, with enormous financial repercussions on health systems worldwide. Ischemic brain injury results from a complex sequence of pathophysiological events that evolve over time. In order to examine the molecular mechanisms underlying middle cerebral artery occlusion (MCAO)‑induced ischemic stroke, the GSE35338 affymetrix microarray data was obtained from the Gene Expression Omnibus database and the differentially expressed genes (DEGs) between samples from patients with MCAO‑induced ischemic stroke and sham controls at various time points were identified. Furthermore, protein‑protein interaction (PPI) networks were constructed by mapping the DEGs into PPI data to identify the pathways that these DEGS are involved in. The results revealed that the expression of 438 DEGs, which are mainly involved in cell death, oxidant reduction, cell cycle and cell‑cell signaling, were altered in MCAO samples. The nodes of CXC motif chemokine 10 (CXCL10) and interleukin‑6 (IL‑6) were large, with degrees of >20. In conclusion, the results suggest that CXCL10 and IL‑6 have important roles in the occurrence and progression of MCAO‑induced ischemic stroke.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Yilmaz G and Granger DN: Cell adhesion molecules and ischemic stroke. Neurol Res. 30:783–793. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Belayev L, Liu Y, et al: Human albumin therapy of acute ischemic stroke: marked neuroprotective efficacy at moderate doses and with a broad therapeutic window. Stroke. 32:553–560. 2001. View Article : Google Scholar

3 

Lakhan SE, Kirchgessner A and Hofer M: Inflammatory mechanisms in ischemic stroke: therapeutic approaches. J Transl Med. 7:972009. View Article : Google Scholar : PubMed/NCBI

4 

Dichgans M: Genetics of ischaemic stroke. Lancet Neurol. 6:149–161. 2007. View Article : Google Scholar

5 

Dirnagl U, Iadecola C and Moskowitz MA: Pathobiology of ischaemic stroke: an integrated view. Trends Neurosci. 22:391–397. 1999. View Article : Google Scholar : PubMed/NCBI

6 

Stoll G, Kleinschnitz C and Nieswandt B: Molecular mechanisms of thrombus formation in ischemic stroke: novel insights and targets for treatment. Blood. 112:3555–3562. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Lo EH, Dalkara T and Moskowitz MA: Mechanisms, challenges and opportunities in stroke. Nat Rev Neurosci. 4:399–415. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Gorelick PB: Stroke prevention therapy beyond antithrombotics: unifying mechanisms in ischemic stroke pathogenesis and implications for therapy: an invited review. Stroke. 33:862–875. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Zamanian JL, Xu L, Foo LC, et al: Genomic analysis of reactive astrogliosis. J Neurosci. 32:6391–6410. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Irizarry RA, Hobbs B, Collin F, et al: Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics. 4:249–264. 2003. View Article : Google Scholar

11 

Gautier L, Cope L, Bolstad BM and Irizarry RA: affy - analysis of Affymetrix GeneChip data at the probe level. Bioinformatics. 20:307–315. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Ihaka R and Gentleman R: R: A language for data analysis and graphics. J Comput Graph Stat. 5:299–314. 1996.

13 

Smyth GK: Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol. 3:32004.PubMed/NCBI

14 

Hulsegge I, Kommadath A and Smits MA: Globaltest and GOEAST: two different approaches for Gene Ontology analysis. BMC Proc. 3(Suppl 4): S102009. View Article : Google Scholar : PubMed/NCBI

15 

Ogata H, Goto S, Sato K, et al: KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res. 27:29–34. 1999. View Article : Google Scholar : PubMed/NCBI

16 

Huang da W, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 4:44–57. 2009.PubMed/NCBI

17 

Szklarczyk D, Franceschini A, Kuhn M, et al: The STRING database in 2011: functional interaction networks of proteins, globally integrated and scored. Nucleic Acids Res. 39:D561–D568. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Shannon P, Markiel A, Ozier O, et al: Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res. 13:2498–2504. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Bader GD and Hogue CW: An automated method for finding molecular complexes in large protein interaction networks. BMC Bioinformatics. 4:22003. View Article : Google Scholar : PubMed/NCBI

20 

Allen C and Bayraktutan U: Oxidative stress and its role in the pathogenesis of ischaemic stroke. Int J Stroke. 4:461–470. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Mitsios N, Saka M, Krupinski J, et al: A microarray study of gene and protein regulation in human and rat brain following middle cerebral artery occlusion. BMC Neurosci. 8:932007. View Article : Google Scholar : PubMed/NCBI

22 

Belperio JA, Keane MP, Burdick MD, et al: Critical role for CXCR3 chemokine biology in the pathogenesis of bronchiolitis obliterans syndrome. J Immunol. 169:1037–1049. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Hancock WW, Gao W, Csizmadia V, et al: Donor-derived IP-10 initiates development of acute allograft rejection. J Exp Med. 193:975–980. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Xie JH, Nomura N, Lu M, et al: Antibody-mediated blockade of the CXCR3 chemokine receptor results in diminished recruitment of T helper 1 cells into sites of inflammation. J Leukocyte Biol. 73:771–780. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Zhai Y, Shen XD, Gao F, et al: CXCL10 regulates liver innate immune response against ischemia and reperfusion injury. Hepatology. 47:207–214. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Rappert A, Bechmann I, Pivneva T, et al: CXCR3-dependent microglial recruitment is essential for dendrite loss after brain lesion. J Neurosci. 24:8500–8509. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Sui Y, Potula R, Dhillon N, et al: Neuronal apoptosis is mediated by CXCL10 overexpression in simian human immunodeficiency virus encephalitis. Am J Pathol. 164:1557–1566. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Dvoriantchikova G, Barakat D, Brambilla R, et al: Inactivation of astroglial NF-kappa B promotes survival of retinal neurons following ischemic injury. Eur J Neurosci. 30:175–185. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Ubogu EE, Cossoy MB and Ransohoff RM: The expression and function of chemokines involved in CNS inflammation. Trends PharMCAOl Sci. 27:48–55. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Camargo CA Jr, Madden JF, Gao W, Selvan RS and Clavien P: Interleukin-6 protects liver against warm ischemia/reperfusion injury and promotes hepatocyte proliferation in the rodent. Hepatology. 26:1513–1520. 1997. View Article : Google Scholar : PubMed/NCBI

31 

Yun S, Wu CY, Deng HM, et al: Synergistic effect of -174 G/C polymorphism of the interleukin-6 gene promoter and 469 E/K polymorphism of the intercellular adhesion molecule-1 gene in the populations of the Han nationality in Shenzhen with cerebral infarction. Proceeding of Clinical Medicine. 24:584–587. 2009.

32 

Finkel MS, Oddis CV, Jacob TD, et al: Negative inotropic effects of cytokines on the heart mediated by nitric oxide. Science. 257:387–389. 1992. View Article : Google Scholar : PubMed/NCBI

33 

Ono K, Matsumori A, Shioi T, Furukawa Y and Sasayama S: Cytokine gene expression after myocardial infarction in rat hearts possible implication in left ventricular remodeling. Circulation. 98:149–156. 1998. View Article : Google Scholar : PubMed/NCBI

34 

Hojo Y, Ikeda U, Takahashi M and Shimada K: Increased levels of monocyte-related cytokines in patients with unstable angina. Atherosclerosis. 161:403–408. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Plutzky J: Inflammatory pathways in atherosclerosis and acute coronary syndromes. Am J Cardiol. 88:10K–15K. 2001. View Article : Google Scholar : PubMed/NCBI

36 

Vila N, Castillo J, Dávalos A and Chamorro A: Proinflammatory cytokines and early neurological worsening in ischemic stroke. Stroke. 31:2325–2329. 2000. View Article : Google Scholar : PubMed/NCBI

37 

Castellanos M, Castillo J, García MM, et al: Inflammation-mediated damage in progressing lacunar infarctions a potential therapeutic target. Stroke. 33:982–987. 2002. View Article : Google Scholar : PubMed/NCBI

38 

Flex A, Gaetani E, Papaleo P, et al: Proinflammatory genetic profiles in subjects with history of ischemic stroke. Stroke. 35:2270–2275. 2004. View Article : Google Scholar : PubMed/NCBI

39 

Mergenthaler P, Dirnagl U and Meisel A: Pathophysiology of stroke: lessons from animal models. Metab Brain Dis. 19:151–167. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Quan Z, Quan Y, Wei B, Fang D, Yu W, Jia H, Quan W, Liu Y and Wang Q: Protein‑protein interaction network and mechanism analysis in ischemic stroke. Mol Med Rep 11: 29-36, 2015.
APA
Quan, Z., Quan, Y., Wei, B., Fang, D., Yu, W., Jia, H. ... Wang, Q. (2015). Protein‑protein interaction network and mechanism analysis in ischemic stroke. Molecular Medicine Reports, 11, 29-36. https://doi.org/10.3892/mmr.2014.2696
MLA
Quan, Z., Quan, Y., Wei, B., Fang, D., Yu, W., Jia, H., Quan, W., Liu, Y., Wang, Q."Protein‑protein interaction network and mechanism analysis in ischemic stroke". Molecular Medicine Reports 11.1 (2015): 29-36.
Chicago
Quan, Z., Quan, Y., Wei, B., Fang, D., Yu, W., Jia, H., Quan, W., Liu, Y., Wang, Q."Protein‑protein interaction network and mechanism analysis in ischemic stroke". Molecular Medicine Reports 11, no. 1 (2015): 29-36. https://doi.org/10.3892/mmr.2014.2696
Copy and paste a formatted citation
x
Spandidos Publications style
Quan Z, Quan Y, Wei B, Fang D, Yu W, Jia H, Quan W, Liu Y and Wang Q: Protein‑protein interaction network and mechanism analysis in ischemic stroke. Mol Med Rep 11: 29-36, 2015.
APA
Quan, Z., Quan, Y., Wei, B., Fang, D., Yu, W., Jia, H. ... Wang, Q. (2015). Protein‑protein interaction network and mechanism analysis in ischemic stroke. Molecular Medicine Reports, 11, 29-36. https://doi.org/10.3892/mmr.2014.2696
MLA
Quan, Z., Quan, Y., Wei, B., Fang, D., Yu, W., Jia, H., Quan, W., Liu, Y., Wang, Q."Protein‑protein interaction network and mechanism analysis in ischemic stroke". Molecular Medicine Reports 11.1 (2015): 29-36.
Chicago
Quan, Z., Quan, Y., Wei, B., Fang, D., Yu, W., Jia, H., Quan, W., Liu, Y., Wang, Q."Protein‑protein interaction network and mechanism analysis in ischemic stroke". Molecular Medicine Reports 11, no. 1 (2015): 29-36. https://doi.org/10.3892/mmr.2014.2696
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team