Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2015 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2015 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Ardisia crenata extract stimulates melanogenesis in B16F10 melanoma cells through inhibiting ERK1/2 and Akt activation

  • Authors:
    • Cheng Yao
    • Cheng Long Jin
    • Jang‑Hee Oh
    • Inn Gyung Oh
    • Chi‑Hyun Park
    • Jin Ho Chung
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea
  • Pages: 653-657
    |
    Published online on: October 17, 2014
       https://doi.org/10.3892/mmr.2014.2697
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Melanin protects the skin against ultraviolet radiation by scattering incoming light and absorbing diverse free radicals. Agents that increase melanin synthesis in melanocytes may reduce the risk of photodamage and skin cancer. The present study investigated the effect of a methanol extract of Ardisia crenata (AC) on melanogenesis in B16F10 cells. Treatment of cultured B16F10 cells with AC extract (10, 20 and 40 µg/ml) stimulated an increase in melanin levels in a concentration‑dependent manner, without cytotoxicity. Tyrosinase is key in the regulation of melanin production, thus the effect of AC extract on tyrosinase activity and protein expression was analyzed. AC extract was observed to significantly increase tyrosinase activity and protein expression in B16F10 cells. Furthermore, AC extract was found to markedly increase the protein expression of microphthalmia‑associated transcription factor, which is an important transcription factor involved in tyrosinase gene expression. In addition, AC extract (40 µg/ml) was observed to suppress the activation of extracellular signal‑regulated kinase (ERK) and Akt, which negatively regulate melanin synthesis in B16F10 cells. In conclusion, to the best of our knowledge, the present study is the first to show that a methanol extract of AC stimulates melanogenesis by increasing tyrosinase expression via the inhibition of ERK and Akt. Thus, methanol extract of AC may be a potential treatment for hypopigmentation diseases and may be a candidate for skin‑tanning cosmetic products.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Hearing VJ: Biogenesis of pigment granules: a sensitive way to regulate melanocyte function. J Dermatol Sci. 37:3–14. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Brenner M and Hearing VJ: The protective role of melanin against UV damage in human skin. Photochem Photobiol. 84:539–549. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Slominski A, Tobin DJ, Shibahara S and Wortsman J: Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol Rev. 84:1155–1228. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Slominski A: Neuroendocrine activity of the melanocyte. Exp Dermatol. 18:760–763. 2009. View Article : Google Scholar

5 

del Marmol V and Beermann F: Tyrosinase and related proteins in mammalian pigmentation. FEBS Lett. 381:165–168. 1996.PubMed/NCBI

6 

Hearing VJ and Jiménez M: Mammalian tyrosinase - the critical regulatory control point in melanocyte pigmentation. Int J Biochem. 19:1141–1147. 1987. View Article : Google Scholar : PubMed/NCBI

7 

Bentley NJ, Eisen T and Goding CR: Melanocyte-specific expression of the human tyrosinase promoter: activation by the microphthalmia gene product and role of the initiator. Mol Cell Biol. 14:7996–8006. 1994.PubMed/NCBI

8 

Buscà R and Ballotti R: Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment Cell Res. 13:60–69. 2000.PubMed/NCBI

9 

Saito H, Yasumoto K, Takeda K, Takahashi K, Yamamoto H and Shibahara S: Microphthalmia-associated transcription factor in the Wnt signaling pathway. Pigment Cell Res. 16:261–265. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Widlund HR and Fisher DE: Microphthalamia-associated transcription factor: a critical regulator of pigment cell development and survival. Oncogene. 22:3035–3041. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Koo JH, Kim HT, Yoon HY, et al: Effect of xanthohumol on melanogenesis in B16 melanoma cells. Exp Mol Med. 40:313–319. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Jiang Z, Li S, Liu Y, Deng P, Huang J and He G: Sesamin induces melanogenesis by microphthalmia-associated transcription factor and tyrosinase up-regulation via cAMP signaling pathway. Acta Biochim Biophys Sin (Shanghai). 43:763–770. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Hamid MA, Sarmidi MR and Park CS: Mangosteen leaf extract increases melanogenesis in B16F1 melanoma cells by stimulating tyrosinase activity in vitro and by up-regulating tyrosinase gene expression. Int J Mol Med. 29:209–217. 2012.PubMed/NCBI

14 

Oka M, Nagai H, Ando H, et al: Regulation of melanogenesis through phosphatidylinositol 3-kinase-Akt pathway in human G361 melanoma cells. J Invest Dermatol. 115:699–703. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Kim DS, Kim SY, Chung JH, Kim KH, Eun HC and Park KC: Delayed ERK activation by ceramide reduces melanin synthesis in human melanocytes. Cell Signal. 14:779–785. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Villareal MO, Han J, Matsuyama K, et al: Lupenone from Erica multiflora leaf extract stimulates melanogenesis in B16 murine melanoma cells through the inhibition of ERK1/2 activation. Planta Med. 79:236–243. 2013.

17 

Galus R, Niderla J, Sladowski D, Sajjad E, Włodarski K and Jóźwiak J: Fluvastatin increases tyrosinase synthesis induced by alpha-melanocyte-stimulating hormone in B16F10 melanoma cells. Pharmacol Rep. 62:164–169. 2010. View Article : Google Scholar

18 

Grimes PE: New insights and new therapies in vitiligo. JAMA. 293:730–735. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Moreira CG, Horinouchi CD, Souza-Filho CS, et al: Hyperpigmentant activity of leaves and flowers extracts of Pyrostegia venusta on murine B16F10 melanoma. J Ethnopharmacol. 141:1005–1011. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Chaabane F, Pinon A, Simon A, Ghedira K and Chekir-Ghedira L: Phytochemical potential of Daphne gnidium in inhibiting growth of melanoma cells and enhancing melanogenesis of B16-F0 melanoma. Cell Biochem Funct. 31:460–467. 2013. View Article : Google Scholar

21 

Maotian W, Xiongtai G, Xiuwen H and Shanhai H: A new triterpenoid saponin from Ardisia crenata. Planta Med. 58:205–207. 1992. View Article : Google Scholar

22 

Jia Z, Koike K, Ohmoto T and Ni M: Triterpenoid saponins from Ardisia crenata. Phytochemistry. 37:1389–1396. 1994. View Article : Google Scholar

23 

Wang X, Tang S, Zhai H and Duan H: Studies on anti-tumor metastatic constituents from Ardisia crenata. Zhongguo Zhong Yao Za Zhi. 36:881–885. 2011.(In Chinese).

24 

Zaima K, Deguchi J, Matsuno Y, Kaneda T, Hirasawa Y and Morita H: Vasorelaxant effect of FR900359 from Ardisia crenata on rat aortic artery. J Nat Med. 67:196–201. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Yao C, Oh JH, Oh IG, Park CH and Chung JH: [6]-Shogaol inhibits melanogenesis in B16 mouse melanoma cells through activation of the ERK pathway. Acta Pharmacol Sin. 34:289–294. 2013.

26 

Buscà R, Bertolotto C, Ortonne JP and Ballotti R: Inhibition of the phosphatidylinositol 3-kinase/p70(S6)-kinase pathway induces B16 melanoma cell differentiation. J Biol Chem. 271:31824–31830. 1996.PubMed/NCBI

27 

Englaro W, Bertolotto C, Buscà R, et al: Inhibition of the mitogen-activated protein kinase pathway triggers B16 melanoma cell differentiation. J Biol Chem. 273:9966–9970. 1998. View Article : Google Scholar : PubMed/NCBI

28 

Cowley S, Paterson H, Kemp P and Marshall CJ: Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells. Cell. 77:841–852. 1994. View Article : Google Scholar : PubMed/NCBI

29 

Sale EM, Atkinson PG and Sale GJ: Requirement of MAP kinase for differentiation of fibroblasts to adipocytes, for insulin activation of p90 S6 kinase and for insulin or serum stimulation of DNA synthesis. EMBO J. 14:674–684. 1995.PubMed/NCBI

30 

Wu M, Hemesath TJ, Takemoto CM, et al: c-Kit triggers dual phosphorylations, which couple activation and degradation of the essential melanocyte factor Mi. Genes Dev. 14:301–312. 2000.PubMed/NCBI

31 

Ahmad S, Singh N and Glazer RI: Role of AKT1 in 17beta-estradiol- and insulin-like growth factor I (IGF-I)-dependent proliferation and prevention of apoptosis in MCF-7 breast carcinoma cells. Biochem Pharmacol. 58:425–430. 1999. View Article : Google Scholar : PubMed/NCBI

32 

Tang Y, Zhou H, Chen A, Pittman RN and Field J: The Akt proto-oncogene links Ras to Pak and cell survival signals. J Biol Chem. 275:9106–9109. 2000. View Article : Google Scholar : PubMed/NCBI

33 

Khaled M, Larribere L, Bille K, Ortonne JP, Ballotti R and Bertolotto C: Microphthalmia associated transcription factor is a target of the phosphatidylinositol-3-kinase pathway. J Invest Dermatol. 121:831–836. 2003. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yao C, Jin CL, Oh JH, Oh IG, Park CH and Chung JH: Ardisia crenata extract stimulates melanogenesis in B16F10 melanoma cells through inhibiting ERK1/2 and Akt activation. Mol Med Rep 11: 653-657, 2015.
APA
Yao, C., Jin, C.L., Oh, J., Oh, I.G., Park, C., & Chung, J.H. (2015). Ardisia crenata extract stimulates melanogenesis in B16F10 melanoma cells through inhibiting ERK1/2 and Akt activation. Molecular Medicine Reports, 11, 653-657. https://doi.org/10.3892/mmr.2014.2697
MLA
Yao, C., Jin, C. L., Oh, J., Oh, I. G., Park, C., Chung, J. H."Ardisia crenata extract stimulates melanogenesis in B16F10 melanoma cells through inhibiting ERK1/2 and Akt activation". Molecular Medicine Reports 11.1 (2015): 653-657.
Chicago
Yao, C., Jin, C. L., Oh, J., Oh, I. G., Park, C., Chung, J. H."Ardisia crenata extract stimulates melanogenesis in B16F10 melanoma cells through inhibiting ERK1/2 and Akt activation". Molecular Medicine Reports 11, no. 1 (2015): 653-657. https://doi.org/10.3892/mmr.2014.2697
Copy and paste a formatted citation
x
Spandidos Publications style
Yao C, Jin CL, Oh JH, Oh IG, Park CH and Chung JH: Ardisia crenata extract stimulates melanogenesis in B16F10 melanoma cells through inhibiting ERK1/2 and Akt activation. Mol Med Rep 11: 653-657, 2015.
APA
Yao, C., Jin, C.L., Oh, J., Oh, I.G., Park, C., & Chung, J.H. (2015). Ardisia crenata extract stimulates melanogenesis in B16F10 melanoma cells through inhibiting ERK1/2 and Akt activation. Molecular Medicine Reports, 11, 653-657. https://doi.org/10.3892/mmr.2014.2697
MLA
Yao, C., Jin, C. L., Oh, J., Oh, I. G., Park, C., Chung, J. H."Ardisia crenata extract stimulates melanogenesis in B16F10 melanoma cells through inhibiting ERK1/2 and Akt activation". Molecular Medicine Reports 11.1 (2015): 653-657.
Chicago
Yao, C., Jin, C. L., Oh, J., Oh, I. G., Park, C., Chung, J. H."Ardisia crenata extract stimulates melanogenesis in B16F10 melanoma cells through inhibiting ERK1/2 and Akt activation". Molecular Medicine Reports 11, no. 1 (2015): 653-657. https://doi.org/10.3892/mmr.2014.2697
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team