Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2015 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2015 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Cathepsin L is involved in proliferation and invasion of ovarian cancer cells

  • Authors:
    • Luosheng Zhang
    • Lixia Wei
    • Guanzhu Shen
    • Benfu He
    • Wei Gong
    • Na Min
    • Li Zhang
    • Yuxin Duan
    • Jingran Xie
    • Huiyan Luo
    • Xingwang Gao
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The 458th Hospital of PLA, Guangzhou, Guangdong 510602, P.R. China, Department of Oncology, Sun Yat‑sen University Cancer Center, Guangzhou, Guangdong 510000, P.R. China, Department of Oncology, The 421th Hospital of PLA, Guangzhou, Guangdong 510000, P.R. China
  • Pages: 468-474
    |
    Published online on: October 20, 2014
       https://doi.org/10.3892/mmr.2014.2706
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Cathepsin L (CTSL) is a lysosomal cysteine protease that has been found to be overexpressed in ovarian cancer (OC). The aim of the present study was to investigate the possible involvement of CTSL in the development of OC. In this study, RNA interference with a CTSL small hairpin RNA (CTSL‑shRNA), and a plasmid carrying CTSL were used to identify the effects of this enzyme on the regulation of the malignant behavior of OC cells. OV‑90 and SKOV3 human ovarian cancer cell lines were selected as cell models in vitro and in vivo. The results showed that downregulation of CTSL significantly inhibits the proliferative and invasive capability of SKOV3 cells, and that upregulation of CTSL in OV‑90 cells leads to opposite effects. Compared with parental OC cells, cells in which CTSL was silenced exhibited a reduced capacity to develop into tumors in nude mice, while the growth of tumor xenografts derived from these cells was markedly constrained. In conclusion, the results suggested that CTSL contributes to the proliferation and metastasis of OC, and that CTSL may be a novel molecular target for OC treatment.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

American Cancer Society. Cancer Facts and Figures 2014. Atlanta, Ga: American Cancer Society; 2014 Last accessed May 21, 2014

2 

Su Z, Graybill WS and Zhu Y: Detection and monitoring of ovarian cancer. Clin Chim Acta. 415:341–345. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Turk V, Stoka V, Vasiljeva O, et al: Cysteine cathepsins: from structure, function and regulation to new frontiers. Biochim Biophys Acta. 1824:68–88. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Gocheva V and Joyce JA: Cysteine cathepsins and the cutting edge of cancer invasion. Cell Cycle. 6:60–64. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Mason SD and Joyce JA: Proteolytic networks in cancer. Trends Cell Biol. 21:228–237. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Conus S and Simon HU: Cathepsins and their involvement in immune responses. Swiss Med Wkly. 140:w130422010.PubMed/NCBI

7 

Krepela E: Cysteine proteinases in tumor cell growth and apoptosis. Neoplasma. 48:332–349. 2001.PubMed/NCBI

8 

Mohamed MM and Sloane BF: Cysteine cathepsins: multifunctional enzymes in cancer. Nat Rev Cancer. 6:764–775. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Duffy MJ: The role of proteolytic enzymes in cancer invasion and metastasis. Clin Exp Metastasis. 10:145–155. 1992. View Article : Google Scholar : PubMed/NCBI

10 

Mullins SR, Sameni M, Blum G, Bogyo M, Sloane BF and Moin K: Three-dimensional cultures modeling premalignant progression of human breast epithelial cells: role of cysteine cathepsins. Biol Chem. 393:1405–1416. 2012. View Article : Google Scholar

11 

Rao Q, Cheng L, Xia QY, et al: Cathepsin K expression in a wide spectrum of perivascular epithelioid cell neoplasms (PEComas): a clinicopathological study emphasizing extrarenal PEComas. Histopathology. 62:642–650. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Jevnikar Z, Rojnik M, Jamnik P, Doljak B, Fonovic UP and Kos J: Cathepsin H mediates the processing of talin and regulates migration of prostate cancer cells. J Biol Chem. 288:2201–2209. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Nakashima T, Yasumatsu R, Masuda M, Clayman GL and Komune S: Prognostic value of cathepsin L and its inhibitor headpin in oral squamous cell carcinoma. J Laryngol Otol. 126:1134–1137. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Bar-Sela G, Kaplan-Cohen V, Ilan N, Vlodavsky I and Ben-Izhak O: Heparanase expression in nasopharyngeal carcinoma inversely correlates with patient survival. Histopathology. 49:188–193. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Zhang W, Hu XX, Yang XZ, et al: Combined detection of serum matrix metalloproteinase 9, acetyl heparinase and cathepsin L in diagnosis of ovarian cancer. Chin J Cancer Res. 24:67–71. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Sivaparvathi M, Yamamoto M, Nicolson GL, et al: Expression and immunohistochemical localization of cathepsin L during the progression of human gliomas. Clin Exp Metastasis. 14:27–34. 1996. View Article : Google Scholar : PubMed/NCBI

17 

Tumminello FM, Leto G, Pizzolanti G, et al: Cathepsin D, B and L circulating levels as prognostic markers of malignant progression. Anticancer Res. 16:2315–2319. 1996.PubMed/NCBI

18 

Friedrich B, Jung K, Lein M, et al: Cathepsins B, H, L and cysteine protease inhibitors in malignant prostate cell lines, primary cultured prostatic cells and prostatic tissue. Eur J Cancer. 35:138–144. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Svatek RS, Karam J, Karakiewicz PI, et al: Role of urinary cathepsin B and L in the detection of bladder urothelial cell carcinoma. J Urol. 179:478–484. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Sheahan K, Shuja S and Murnane MJ: Cysteine protease activities and tumor development in human colorectal carcinoma. Cancer Res. 49:3809–3814. 1989.PubMed/NCBI

21 

Watanabe M, Higashi T, Hashimoto M, et al: Elevation of tissue cathepsin B and L activities in gastric cancer. Hepatogastroenterology. 34:120–122. 1987.PubMed/NCBI

22 

Gabrijelcic D, Svetic B, Spaić D, et al: Cathepsins B, H and L in human breast carcinoma. Eur J Clin Chem Clin Biochem. 30:69–74. 1992.PubMed/NCBI

23 

Niedergethmann M, Wostbrock B, Sturm JW, Willeke F, Post S and Hildenbrand R: Prognostic impact of cysteine proteases cathepsin B and cathepsin L in pancreatic adenocarcinoma. Pancreas. 29:204–211. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Casson AG, Wilson SM, McCart JA, et al: ras mutation and expression of the ras-regulated genes osteopontin and cathepsin L in human esophageal cancer. Int J Cancer. 72:739–745. 1997. View Article : Google Scholar : PubMed/NCBI

25 

Samaiya M, Bakhshi S, Shukla AA, Kumar L and Chauhan SS: Epigenetic regulation of cathepsin L expression in chronic myeloid leukaemia. J Cell Mol Med. 15:2189–2199. 2011. View Article : Google Scholar : PubMed/NCBI

26 

De Luca A, Maiello MR, D’Alessio A, Pergameno M and Normanno N: The RAS/RAF/MEK/ERK and the PI3K/AKT signalling pathways: role in cancer pathogenesis and implications for therapeutic approaches. Expert Opin Ther Targets. 16(Suppl 2): S17–S27. 2012.PubMed/NCBI

27 

Zhang W, Ling D, Tan J, Zhang J and Li L: Expression of urokinase plasminogen activator and plasminogen activator inhibitor type-1 in ovarian cancer and its clinical significance. Oncol Rep. 29:637–645. 2013.PubMed/NCBI

28 

Goldman S and Shalev E: MMPS and TIMPS in ovarian physiology and pathophysiology. Front Biosci. 9:2474–2483. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Wei L, Shen G, He B, Gong W, Min N, Zhang L, Duan Y, Xie J, Luo H, Luo H, et al: Cathepsin L is involved in proliferation and invasion of ovarian cancer cells. Mol Med Rep 11: 468-474, 2015.
APA
Zhang, L., Wei, L., Shen, G., He, B., Gong, W., Min, N. ... Gao, X. (2015). Cathepsin L is involved in proliferation and invasion of ovarian cancer cells. Molecular Medicine Reports, 11, 468-474. https://doi.org/10.3892/mmr.2014.2706
MLA
Zhang, L., Wei, L., Shen, G., He, B., Gong, W., Min, N., Zhang, L., Duan, Y., Xie, J., Luo, H., Gao, X."Cathepsin L is involved in proliferation and invasion of ovarian cancer cells". Molecular Medicine Reports 11.1 (2015): 468-474.
Chicago
Zhang, L., Wei, L., Shen, G., He, B., Gong, W., Min, N., Zhang, L., Duan, Y., Xie, J., Luo, H., Gao, X."Cathepsin L is involved in proliferation and invasion of ovarian cancer cells". Molecular Medicine Reports 11, no. 1 (2015): 468-474. https://doi.org/10.3892/mmr.2014.2706
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Wei L, Shen G, He B, Gong W, Min N, Zhang L, Duan Y, Xie J, Luo H, Luo H, et al: Cathepsin L is involved in proliferation and invasion of ovarian cancer cells. Mol Med Rep 11: 468-474, 2015.
APA
Zhang, L., Wei, L., Shen, G., He, B., Gong, W., Min, N. ... Gao, X. (2015). Cathepsin L is involved in proliferation and invasion of ovarian cancer cells. Molecular Medicine Reports, 11, 468-474. https://doi.org/10.3892/mmr.2014.2706
MLA
Zhang, L., Wei, L., Shen, G., He, B., Gong, W., Min, N., Zhang, L., Duan, Y., Xie, J., Luo, H., Gao, X."Cathepsin L is involved in proliferation and invasion of ovarian cancer cells". Molecular Medicine Reports 11.1 (2015): 468-474.
Chicago
Zhang, L., Wei, L., Shen, G., He, B., Gong, W., Min, N., Zhang, L., Duan, Y., Xie, J., Luo, H., Gao, X."Cathepsin L is involved in proliferation and invasion of ovarian cancer cells". Molecular Medicine Reports 11, no. 1 (2015): 468-474. https://doi.org/10.3892/mmr.2014.2706
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team