Reprogramming human adipose tissue stem cells using epidermal keratinocyte extracts

Xie,Feng; Tang,Xinjie; Zhang,Qun; Deng,Chenliang

doi:10.3892/mmr.2014.2711

January-2015 Volume 11 Issue 1

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

January-2015 Volume 11 Issue 1

Full Size Image

Article Open Access

Reprogramming human adipose tissue stem cells using epidermal keratinocyte extracts

Authors:
- Feng Xie
- Xinjie Tang
- Qun Zhang
- Chenliang Deng
View Affiliations / Copyright

Affiliations: Department of Plastic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University, Shanghai 200011, P.R. China, Department of Plastic and Reconstructive Surgery, Huashan Hospital, Fudan University School of Medicine, Shanghai 200040, P.R. China, Department of Plastic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, P.R. China

Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 182-188
|
Published online on: October 21, 2014

https://doi.org/10.3892/mmr.2014.2711
Expand metrics +

Abstract

Human adipose tissue stem cells (ATSCs) can differentiate into various types of cell in response to lineage‑specific induction factors. Reprogramming cells using nuclear and cytoplasmic extracts derived from another type of somatic cell is an effective method of producing specific types of differentiated cell. In the present study, the ability of reprogrammed ATSCs to acquire epidermal keratinocyte properties following transient exposure to epidermal keratinocyte extracts was demonstrated. Reversibly permeabilized ATSCs were incubated for 1 h in nuclear and cytoplasmic extracts from epidermal keratinocytes, resealed with CaCl2 and cultured. ATSC reprogramming is demonstrated by nuclear uptake of epidermal keratinocyte extracts. After one week of exposure to extracts, ATSCs underwent changes in cell morphology, cell‑specific genes were activated, and epidermal keratinocyte markers including K19 and K1/K10 (markers of stem cells and terminally differentiated keratinocytes, respectively) were expressed. This study indicates that the reprogramming of ATSCs using nuclear and cytoplasmic extracts from epidermal keratinocytes is a viable option for the production of specific types of cell.

View Figures

View References

1	Nayak S, Dey S and Kundu SC: Skin equivalent tissue-engineered construct: co-cultured fibroblasts/keratinocytes on 3D matrices of sericin hope cocoons. PLoS One. 8:e747792013. View Article : Google Scholar : PubMed/NCBI
2	Li H, Chu Y, Zhang Z, et al: Construction of bilayered tissue-engineered skin with human amniotic mesenchymal cells and human amniotic epithelial cells. Artif Organs. 36:911–919. 2012. View Article : Google Scholar : PubMed/NCBI
3	Nie X, Zhang JY, Cai KJ, et al: Cosmetic improvement in various acute skin defects treated with tissue-engineered skin. Artif Organs. 31:703–710. 2007. View Article : Google Scholar : PubMed/NCBI
4	Tang X, Sheng L, Xie F and Zhang Q: Differentiation of bone marrow-derived mesenchymal stem cells into chondrocytes using chondrocyte extract. Mol Med Rep. 6:745–749. 2012.PubMed/NCBI
5	Håkelien AM, Landsverk HB, Robl JM, Skålhegg BS and Collas P: Reprogramming fibroblasts to express T-cell functions using cell extracts. Nat Biotechnol. 20:460–466. 2002.PubMed/NCBI
6	Gaustad KG, Boquest AC, Anderson BE, Gerdes AM and Collas P: Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytes. Biochem Biophys Res Commun. 314:420–427. 2004. View Article : Google Scholar : PubMed/NCBI
7	Walev I, Bhakdi SC, Hofmann F, et al: Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O. Proc Natl Acad Sci USA. 98:3185–3190. 2001. View Article : Google Scholar : PubMed/NCBI
8	Collas P and Håkelien AM: Teaching cells new tricks. Trends Biotechnol. 21:354–361. 2003. View Article : Google Scholar : PubMed/NCBI
9	Zuk PA, Zhu M, Ashjian P, et al: Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 13:4279–4295. 2002.PubMed/NCBI
10	Cousin B, André M, Arnaud E, Pénicaud L and Casteilla L: Reconstitution of lethally irradiated mice by cells isolated from adipose tissue. Biochem Biophys Res Commun. 301:1016–1022. 2003. View Article : Google Scholar : PubMed/NCBI
11	Håkelien AM, Gaustad KG and Collas P: Transient alteration of cell fate using a nuclear and cytoplasmic extract of an insulinoma cell line. Biochem Biophys Res Commun. 316:834–841. 2004.PubMed/NCBI
12	Wernig M, Meissner A, Foreman R, et al: In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature. 448:318–324. 2007. View Article : Google Scholar : PubMed/NCBI
13	Herzog EL, Chai L and Krause DS: Plasticity of marrow-derived stem cells. Blood. 102:3483–3493. 2003. View Article : Google Scholar : PubMed/NCBI
14	Landsverk HB, Håkelien AM, Küntziger T, Robl JM, Skålhegg BS and Collas P: Reprogrammed gene expression in a somatic cell-free extract. EMBO Rep. 3:384–389. 2002. View Article : Google Scholar : PubMed/NCBI
15	Maison C, Bailly D, Peters AH, et al: Higher-order structure in pericentric heterochromatin involves a distinct pattern of histone modification and an RNA component. Nat Genet. 30:329–334. 2002. View Article : Google Scholar : PubMed/NCBI
16	Sleutels F, Zwart R and Barlow DP: The non-coding Air RNA is required for silencing autosomal imprinted genes. Nature. 415:810–813. 2002. View Article : Google Scholar : PubMed/NCBI
17	Mikkelsen TS, Hanna J, Zhang X, et al: Dissecting direct reprogramming through integrative genomic analysis. Nature. 454:49–55. 2008. View Article : Google Scholar : PubMed/NCBI
18	Huangfu D, Osafune K, Maehr R, et al: Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nat Biotechnol. 26:1269–1275. 2008. View Article : Google Scholar : PubMed/NCBI
19	Huangfu D, Maehr R, Guo W, et al: Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. Nat Biotechnol. 26:795–797. 2008. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Reprogramming human adipose tissue stem cells using epidermal keratinocyte extracts

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Related Articles