Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2015 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2015 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of vitamin C administration on hydrogen peroxide‑induced cytotoxicity in periodontal ligament cells

  • Authors:
    • Wenlei Wu
    • Nanfei Yang
    • Xiujing Feng
    • Tingzhe Sun
    • Pingping Shen
    • Weibin Sun
  • View Affiliations / Copyright

    Affiliations: Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210093, P.R. China, School of Life Sciences, Anqing Normal University, Anqing, Anhui 246000, P.R. China
  • Pages: 242-248
    |
    Published online on: October 21, 2014
       https://doi.org/10.3892/mmr.2014.2712
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Periodontitis is a disease, which is associated with chronic inflammation and leads to significant destruction of periodontal tissues. Periodontal ligament cells (PDLCs) constitute the largest cell population in PDL tissues and a considerable body of evidence has demonstrated an association between oxidative stress and the progression of periodontitis. However, the effects on PDLCs exposed to hydrogen peroxide (H2O2) and the molecular mechanisms by which H2O2 affects periodontitis remain to be elucidated. In the present study, the potential cytotoxic effect of H2O2 and the antioxidative function of vitamin C (Vc) in PDLCs were investigated. The results demonstrated that H2O2 treatment decreased the viability of PDLCs. The decreased PDLC viability was primarily induced by apoptosis, which was evidenced by cleaved caspases‑3, caspases‑9 and poly (ADP‑ribose) polymerase. Following optimal Vc addition, the proapoptotic effects of H2O2 were partially antagonized. Taken together, the present study demonstrated that H2O2 primarily induced the apoptosis of PDLCs and that these adverse effects were partially rescued following treatment with Vc. These results revealed how H2O2 promotes the progression of periodontitis and provide an improved understanding of the reversal effect of antioxidant treatment. Therefore, optimal Vc administration may provide a potentially effective technique in periodontal therapy.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Fialkow L, Wang Y and Downey GP: Reactive oxygen and nitrogen species as signaling molecules regulating neutrophil function. Free Radic Biol Med. 42:153–164. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Chapple IL and Matthews JB: The role of reactive oxygen and antioxidant species in periodontal tissue destruction. Periodontol 2000. 43:160–232. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Newsholme P, Rebelato E, Abdulkader F, Krause M, Carpinelli A and Curi R: Reactive oxygen and nitrogen species generation, antioxidant defenses, and beta-cell function: a critical role for amino acids. J Endocrinol. 214:11–20. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Pihlstrom BL, Michalowicz BS and Johnson NW: Periodontal diseases. Lancet. 366:1809–1820. 2005. View Article : Google Scholar

5 

Nibali L and Donos N: Periodontitis and redox status: a review. Curr Pharm Des. 19:2687–2697. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Wrzaczek M, Brosché M and Kangasjärvi J: ROS signaling loops - production, perception, regulation. Curr Opin Plant Biol. 16:575–582. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Akman S, Canakci V, Kara A, Tozoglu U, Arabaci T and Dagsuyu IM: Therapeutic effects of alpha lipoic acid and vitamin C on alveolar bone resorption after experimental periodontitis in rats: a biochemical, histochemical, and stereologic study. J Periodontol. 84:666–674. 2013. View Article : Google Scholar

8 

Ouyang L, Shi Z, Zhao S, et al: Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis. Cell Prolif. 45:487–498. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Jönsson D, Nebel D, Bratthall G and Nilsson BO: The human periodontal ligament cell: a fibroblast-like cell acting as an immune cell. J Periodontal Res. 46:153–157. 2011.PubMed/NCBI

10 

Somerman MJ, Young MF, Foster RA, Moehring JM, Imm G and Sauk JJ: Characteristics of human periodontal ligament cells in vitro. Arch Oral Biol. 35:241–247. 1990. View Article : Google Scholar : PubMed/NCBI

11 

Burgoyne JR, Oka S, Ale-Agha N and Eaton P: Hydrogen peroxide sensing and signaling by protein kinases in the cardiovascular system. Antioxid Redox Signal. 18:1042–1052. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Novotny GW, Lundh M, Backe MB, et al: Transcriptional and translational regulation of cytokine signaling in inflammatory beta-cell dysfunction and apoptosis. Arch Biochem Biophys. 528:171–184. 2012. View Article : Google Scholar : PubMed/NCBI

13 

D’Aiuto F, Nibali L, Parkar M, Patel K, Suvan J and Donos N: Oxidative stress, systemic inflammation, and severe periodontitis. J Dent Res. 89:1241–1246. 2010.

14 

Lin X, Sun T, Cai M and Shen P: Cell-death-mode switch from necrosis to apoptosis in hydrogen peroxide treated macrophages. Sci China Life Sci. 53:1196–1203. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Tsai CC, Chen HS, Chen SL, et al: Lipid peroxidation: a possible role in the induction and progression of chronic periodontitis. J Periodontal Res. 40:378–384. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Chapple IL, Milward MR and Dietrich T: The prevalence of inflammatory periodontitis is negatively associated with serum antioxidant concentrations. J Nutr. 137:657–664. 2007.PubMed/NCBI

17 

Konopka T, Król K, Kopeć W and Gerber H: Total antioxidant status and 8-hydroxy-2′-deoxyguanosine levels in gingival and peripheral blood of periodontitis patients. Arch Immunol Ther Exp (Warsz). 55:417–422. 2007.

18 

Darveau RP: Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol. 8:481–490. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Kumar D, Moore RM, Elkhwad M, Silver RJ and Moore JJ: Vitamin C exacerbates hydrogen peroxide induced apoptosis and concomitant PGE2 release in amnion epithelial and mesenchymal cells, and in intact amnion. Placenta. 25:573–579. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Nagappan A, Park KI, Park HS, et al: Vitamin C induces apoptosis in AGS cells by down-regulation of 14-3-3sigma via a mitochondrial dependent pathway. Food Chem. 135:1920–1928. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Dhar-Mascareño M, Cárcamo JM and Golde DW: Hypoxia-reoxygenation-induced mitochondrial damage and apoptosis in human endothelial cells are inhibited by vitamin C. Free Radic Biol Med. 38:1311–1322. 2005.PubMed/NCBI

22 

Gobbo MG, Ribeiro DL, Taboga SR, de Almeida EA and Góes RM: Oxidative stress markers and apoptosis in the prostate of diabetic rats and the influence of vitamin C treatment. J Cell Biochem. 113:2223–2233. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Crott JW and Fenech M: Effect of vitamin C supplementation on chromosome damage, apoptosis and necrosis ex vivo. Carcinogenesis. 20:1035–1041. 1999. View Article : Google Scholar : PubMed/NCBI

24 

Hong SW, Jin DH, Hahm ES, et al: Ascorbate (vitamin C) induces cell death through the apoptosis-inducing factor in human breast cancer cells. Oncol Rep. 18:811–815. 2007.PubMed/NCBI

25 

Orzechowski A, Łokociejewska M, Muras P and Hocquette JF: Preconditioning with millimolar concentrations of vitamin C or N-acetylcysteine protects L6 muscle cells insulin-stimulated viability and DNA synthesis under oxidative stress. Life Sci. 71:1793–1808. 2002. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wu W, Yang N, Feng X, Sun T, Shen P and Sun W: Effect of vitamin C administration on hydrogen peroxide‑induced cytotoxicity in periodontal ligament cells. Mol Med Rep 11: 242-248, 2015.
APA
Wu, W., Yang, N., Feng, X., Sun, T., Shen, P., & Sun, W. (2015). Effect of vitamin C administration on hydrogen peroxide‑induced cytotoxicity in periodontal ligament cells. Molecular Medicine Reports, 11, 242-248. https://doi.org/10.3892/mmr.2014.2712
MLA
Wu, W., Yang, N., Feng, X., Sun, T., Shen, P., Sun, W."Effect of vitamin C administration on hydrogen peroxide‑induced cytotoxicity in periodontal ligament cells". Molecular Medicine Reports 11.1 (2015): 242-248.
Chicago
Wu, W., Yang, N., Feng, X., Sun, T., Shen, P., Sun, W."Effect of vitamin C administration on hydrogen peroxide‑induced cytotoxicity in periodontal ligament cells". Molecular Medicine Reports 11, no. 1 (2015): 242-248. https://doi.org/10.3892/mmr.2014.2712
Copy and paste a formatted citation
x
Spandidos Publications style
Wu W, Yang N, Feng X, Sun T, Shen P and Sun W: Effect of vitamin C administration on hydrogen peroxide‑induced cytotoxicity in periodontal ligament cells. Mol Med Rep 11: 242-248, 2015.
APA
Wu, W., Yang, N., Feng, X., Sun, T., Shen, P., & Sun, W. (2015). Effect of vitamin C administration on hydrogen peroxide‑induced cytotoxicity in periodontal ligament cells. Molecular Medicine Reports, 11, 242-248. https://doi.org/10.3892/mmr.2014.2712
MLA
Wu, W., Yang, N., Feng, X., Sun, T., Shen, P., Sun, W."Effect of vitamin C administration on hydrogen peroxide‑induced cytotoxicity in periodontal ligament cells". Molecular Medicine Reports 11.1 (2015): 242-248.
Chicago
Wu, W., Yang, N., Feng, X., Sun, T., Shen, P., Sun, W."Effect of vitamin C administration on hydrogen peroxide‑induced cytotoxicity in periodontal ligament cells". Molecular Medicine Reports 11, no. 1 (2015): 242-248. https://doi.org/10.3892/mmr.2014.2712
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team