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Effect of A disintegrin and metalloproteinase 10 gene silencing on the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113

  • Authors:
    • Yuan Shao
    • Xiao‑Ying Sha
    • Yan‑Xia Bai
    • Fang Quan
    • Sheng‑Li Wu
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, The Sixth Hepatic Disease Ward, The Affiliated Xi'an Eighth Hospital, Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
    Copyright: © Shao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 212-218
    |
    Published online on: October 21, 2014
       https://doi.org/10.3892/mmr.2014.2717
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Abstract

The present study aimed to investigate the effect of A disintegrin and metalloproteinase 10 (ADAM10) gene silencing on the proliferation, migration and invasion of the human tongue squamous cell carcinoma cell line TCA8113. RNA interference was used to knock down the expression of ADAM10 in the TCA8113 cell line and the proliferation, migration and invasive ability of the treated cells were observed in vitro. The expression levels of epidermal growth factor receptor (EGFR) and E‑cadherin in the treated cells were determined by western blot analysis. The proliferation, migration and invasion abilities of cells in the ADAM10 siRNA‑treated group were significantly lower than those in the control groups (P<0.05). In addition, compared with the control groups, the expression levels of EGFR and E‑cadherin in the ADAM10 siRNA‑treated cells were significantly decreased (P<0.05) and increased (P<0.05), respectively. These results suggested that ADAM10 is important in regulating the proliferation, invasion and migra­tion of the human tongue squamous cell carcinoma cell line TCA8113 and that the mechanism may, at least in part, be associated with the upregulation of EGFR and the downregulation of E‑cadherin.
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Copy and paste a formatted citation
Spandidos Publications style
Shao Y, Sha XY, Bai YX, Quan F and Wu SL: Effect of A disintegrin and metalloproteinase 10 gene silencing on the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113. Mol Med Rep 11: 212-218, 2015.
APA
Shao, Y., Sha, X., Bai, Y., Quan, F., & Wu, S. (2015). Effect of A disintegrin and metalloproteinase 10 gene silencing on the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113. Molecular Medicine Reports, 11, 212-218. https://doi.org/10.3892/mmr.2014.2717
MLA
Shao, Y., Sha, X., Bai, Y., Quan, F., Wu, S."Effect of A disintegrin and metalloproteinase 10 gene silencing on the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113". Molecular Medicine Reports 11.1 (2015): 212-218.
Chicago
Shao, Y., Sha, X., Bai, Y., Quan, F., Wu, S."Effect of A disintegrin and metalloproteinase 10 gene silencing on the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113". Molecular Medicine Reports 11, no. 1 (2015): 212-218. https://doi.org/10.3892/mmr.2014.2717
Copy and paste a formatted citation
x
Spandidos Publications style
Shao Y, Sha XY, Bai YX, Quan F and Wu SL: Effect of A disintegrin and metalloproteinase 10 gene silencing on the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113. Mol Med Rep 11: 212-218, 2015.
APA
Shao, Y., Sha, X., Bai, Y., Quan, F., & Wu, S. (2015). Effect of A disintegrin and metalloproteinase 10 gene silencing on the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113. Molecular Medicine Reports, 11, 212-218. https://doi.org/10.3892/mmr.2014.2717
MLA
Shao, Y., Sha, X., Bai, Y., Quan, F., Wu, S."Effect of A disintegrin and metalloproteinase 10 gene silencing on the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113". Molecular Medicine Reports 11.1 (2015): 212-218.
Chicago
Shao, Y., Sha, X., Bai, Y., Quan, F., Wu, S."Effect of A disintegrin and metalloproteinase 10 gene silencing on the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113". Molecular Medicine Reports 11, no. 1 (2015): 212-218. https://doi.org/10.3892/mmr.2014.2717
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