Paris saponin Ⅶ inhibits metastasis by modulating matrix metalloproteinases in colorectal cancer cells

Fan,Lei; Li,Yuhua; Sun,Yang; Yue,Zhenggang; Meng,Jin; Zhang,Xutao; Zhang,Rong; Zhang,Dian; Zhang,Feng; Mei,Qibing

doi:10.3892/mmr.2014.2728

January-2015 Volume 11 Issue 1

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January-2015 Volume 11 Issue 1

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Article

Paris saponin Ⅶ inhibits metastasis by modulating matrix metalloproteinases in colorectal cancer cells

Authors:
- Lei Fan
- Yuhua Li
- Yang Sun
- Zhenggang Yue
- Jin Meng
- Xutao Zhang
- Rong Zhang
- Dian Zhang
- Feng Zhang
- Qibing Mei
View Affiliations / Copyright

Affiliations: Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Collaborative Innovation Center for Chinese Medicine in Qinba Mountains, Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Pharmacy, 309th Hospital of PLA, Beijing 100091, P.R. China
Pages: 705-711
|
Published online on: October 21, 2014

https://doi.org/10.3892/mmr.2014.2728
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Abstract

Metastasis is the main cause of mortality of patients with cancer‑related disease. Targeting the process of metastasis has been proposed as a potential strategy in cancer treatment. Trillium tschonoskii Maxim., a traditional Chinese medicine, is used for the treatment of numerous diseases, including cancer. The current study aimed to determine the anti‑metastatic effect of Paris saponin VII (PS Ⅶ), which was extracted from T. tschonoskii Maxim., using SW620 and LoVo cells, two human metastatic colorectal cancer (CRC) cell lines. The present study conducted cell attachment, wound healing and migration assays to detect the anti‑metastatic effects of PS VII on colorectal cells. In addition, gelatin zymography assay and western blot analysis were used to detect the possible mechanisms involved. The results of this study demonstrated that PS Ⅶ significantly suppresses the viability, attachment, migration and invasive abilities of CRC cells in a concentration‑dependent manner. In addition, PS Ⅶ reduced the expression levels and activity of matrix metalloproteinase (MMP)‑2 and MMP‑9. These data indicate that PS Ⅶ reduces the metastatic capability of CRC cells, possibly via the downregulation of the expression and activity of MMP‑2 and MMP‑9. These results demonstrate a novel therapeutic potential for PS Ⅶ in anti‑metastatic therapy.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Paris saponin Ⅶ inhibits metastasis by modulating matrix metalloproteinases in colorectal cancer cells

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