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Article Open Access

Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression

  • Authors:
    • Shi-Bin Guo
    • Qing Li
    • Zhi-Jun Duan
    • Qiu-Ming Wang
    • Qin Zhou
    • Xiao-Yu Sun
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 0086-116011, P.R. China, Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 0086-116011, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 83-90
    |
    Published online on: October 22, 2014
       https://doi.org/10.3892/mmr.2014.2735
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Abstract

The aim of the present study was to investigate the effects of octreotide treatment on hepatic heme oxygenase‑1 (HO‑1) expression, together with the influence of altered hepatic HO‑1 expression levels on hepatic function and fibrosis in bile duct‑ligated rats. The rats were divided randomly into sham, cirrhotic, cobalt protoporphyrin and octreotide treatment groups. The expression levels of hepatic HO‑1 mRNA were measured by reverse‑transcription polymerase chain reaction, while the protein expression was determined by western blotting and immunohistochemical analysis. Hematoxylin and eosin, and Van Gieson's staining, along with determination of the hydroxyproline content in the liver, were performed to determine the degree of liver fibrosis. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and carboxyhemoglobin (COHb) in arterial blood, and the mean arterial pressure and portal vein pressure were also measured. As compared with the sham group, hepatic HO‑1 mRNA and protein expression levels, serum levels of ALT, AST and TBIL, COHb in arterial blood, hydroxyproline and collagen type I content were all significantly increased in the cirrhotic group. As compared with the cirrhotic group, the octreotide‑treated group exhibited significantly reduced hepatic HO‑1 expression levels, serum levels of ALT, AST and TBIL, COHb in arterial blood and the extent of hepatic fibrosis, whereas the cobalt protoporphyrin group exhibited significantly increased hepatic HO‑1 expression levels, as well as aggravated hepatic function and fibrosis (P<0.05). In conclusion, octreotide inhibited hepatic HO‑1 overexpression in cirrhotic rats, reduced hepatic HO‑1 expression levels to relieve liver injury and attenuated liver fibrosis.
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Copy and paste a formatted citation
Spandidos Publications style
Guo S, Li Q, Duan Z, Wang Q, Zhou Q and Sun X: Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression. Mol Med Rep 11: 83-90, 2015.
APA
Guo, S., Li, Q., Duan, Z., Wang, Q., Zhou, Q., & Sun, X. (2015). Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression. Molecular Medicine Reports, 11, 83-90. https://doi.org/10.3892/mmr.2014.2735
MLA
Guo, S., Li, Q., Duan, Z., Wang, Q., Zhou, Q., Sun, X."Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression". Molecular Medicine Reports 11.1 (2015): 83-90.
Chicago
Guo, S., Li, Q., Duan, Z., Wang, Q., Zhou, Q., Sun, X."Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression". Molecular Medicine Reports 11, no. 1 (2015): 83-90. https://doi.org/10.3892/mmr.2014.2735
Copy and paste a formatted citation
x
Spandidos Publications style
Guo S, Li Q, Duan Z, Wang Q, Zhou Q and Sun X: Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression. Mol Med Rep 11: 83-90, 2015.
APA
Guo, S., Li, Q., Duan, Z., Wang, Q., Zhou, Q., & Sun, X. (2015). Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression. Molecular Medicine Reports, 11, 83-90. https://doi.org/10.3892/mmr.2014.2735
MLA
Guo, S., Li, Q., Duan, Z., Wang, Q., Zhou, Q., Sun, X."Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression". Molecular Medicine Reports 11.1 (2015): 83-90.
Chicago
Guo, S., Li, Q., Duan, Z., Wang, Q., Zhou, Q., Sun, X."Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression". Molecular Medicine Reports 11, no. 1 (2015): 83-90. https://doi.org/10.3892/mmr.2014.2735
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