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Article

Electroacupuncture inhibits apoptosis of splenic lymphocytes in traumatized rats through modulation of the TNF-α/NF-κB signaling pathway

  • Authors:
    • Kun Wang
    • Huaxing Wu
    • Meng Chi
    • Jian Zhang
    • Guonian Wang
    • Hulun Li
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The Cancer Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Department of Endoscopy, The Cancer Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang 150006, P.R. China
  • Pages: 237-241
    |
    Published online on: October 22, 2014
       https://doi.org/10.3892/mmr.2014.2740
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Abstract

Surgical trauma leads to a severe deterioration of the immune system. Electroacupuncture (EA) may improve the immunodeficiency that occurs following surgery; however, the underlying signaling mechanisms require further study. In the present study, 40 rats were equally randomized into four groups: Control; Control + EA; Trauma; Trauma + EA. EA was applied at the ‘Zusanli’ (ST36) and ‘Lanwei’ (Extra37) acupoints, immediately following surgery. The splenic T cells were isolated from the rats 24 h after surgery. The apoptotic rate of the lymphocytes was measured by flow cytometric analysis, and western blotting was used to determine the protein expression levels of caspase‑3, caspase‑8, tumor necrosis factor (TNF)‑α and TNF receptor 1 (TNFR1). The DNA binding activity of nuclear factor (NF)‑κB was determined using Trans‑AM® ELISA‑based kits. The results of the present study showed that surgical trauma induced apoptosis of splenic lymphocytes, and significantly increased the protein expression levels of caspase‑3 and caspase‑8. This was accompanied by increased expression levels of TNF‑α and TNFR1, and a marked reduction in the activity of NF‑κB in splenic T cells. Administration of EA significantly decreased the expression levels of caspase‑3, caspase‑8, TNF‑α and TNFR1, elevated the activity of NF‑κB, and suppressed the apoptotic rate of the lymphocytes. The data suggests that EA may inhibit the apoptosis of splenic lymphocytes induced by surgical trauma, and ameliorate the postoperative immunosuppression. This may be mediated by the downregulation of TNF‑α expression levels and upregulation of the activity of NF‑κB.
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Copy and paste a formatted citation
Spandidos Publications style
Wang K, Wu H, Chi M, Zhang J, Wang G and Li H: Electroacupuncture inhibits apoptosis of splenic lymphocytes in traumatized rats through modulation of the TNF-α/NF-κB signaling pathway. Mol Med Rep 11: 237-241, 2015.
APA
Wang, K., Wu, H., Chi, M., Zhang, J., Wang, G., & Li, H. (2015). Electroacupuncture inhibits apoptosis of splenic lymphocytes in traumatized rats through modulation of the TNF-α/NF-κB signaling pathway. Molecular Medicine Reports, 11, 237-241. https://doi.org/10.3892/mmr.2014.2740
MLA
Wang, K., Wu, H., Chi, M., Zhang, J., Wang, G., Li, H."Electroacupuncture inhibits apoptosis of splenic lymphocytes in traumatized rats through modulation of the TNF-α/NF-κB signaling pathway". Molecular Medicine Reports 11.1 (2015): 237-241.
Chicago
Wang, K., Wu, H., Chi, M., Zhang, J., Wang, G., Li, H."Electroacupuncture inhibits apoptosis of splenic lymphocytes in traumatized rats through modulation of the TNF-α/NF-κB signaling pathway". Molecular Medicine Reports 11, no. 1 (2015): 237-241. https://doi.org/10.3892/mmr.2014.2740
Copy and paste a formatted citation
x
Spandidos Publications style
Wang K, Wu H, Chi M, Zhang J, Wang G and Li H: Electroacupuncture inhibits apoptosis of splenic lymphocytes in traumatized rats through modulation of the TNF-α/NF-κB signaling pathway. Mol Med Rep 11: 237-241, 2015.
APA
Wang, K., Wu, H., Chi, M., Zhang, J., Wang, G., & Li, H. (2015). Electroacupuncture inhibits apoptosis of splenic lymphocytes in traumatized rats through modulation of the TNF-α/NF-κB signaling pathway. Molecular Medicine Reports, 11, 237-241. https://doi.org/10.3892/mmr.2014.2740
MLA
Wang, K., Wu, H., Chi, M., Zhang, J., Wang, G., Li, H."Electroacupuncture inhibits apoptosis of splenic lymphocytes in traumatized rats through modulation of the TNF-α/NF-κB signaling pathway". Molecular Medicine Reports 11.1 (2015): 237-241.
Chicago
Wang, K., Wu, H., Chi, M., Zhang, J., Wang, G., Li, H."Electroacupuncture inhibits apoptosis of splenic lymphocytes in traumatized rats through modulation of the TNF-α/NF-κB signaling pathway". Molecular Medicine Reports 11, no. 1 (2015): 237-241. https://doi.org/10.3892/mmr.2014.2740
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