Inhibition of interleukin‑1β‑stimulated matrix metalloproteinases via the controlled release of interleukin‑1Ra from chitosan microspheres in chondrocytes

  • Authors:
    • Pang‑Hu Zhou
    • Bei‑Lei Ma
    • Lei Shi
    • Ting Xie
    • Bo Qiu
  • View Affiliations

  • Published online on: October 22, 2014     https://doi.org/10.3892/mmr.2014.2743
  • Pages: 555-560
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the study was to determine whether the controlled release of interleukin (IL)‑1Ra from chitosan (CS) microspheres inhibits the IL‑1β‑stimulated production of matrix metalloproteinases (MMPs) in chondrocytes. The CS‑IL‑1Ra microspheres were fabricated by an emulsification method using sodium tripolyphosphate as a crosslinker, and the controlled release of IL‑1Ra was determined using an enzyme‑linked immunosorbent assay. IL‑1β was added to normal rat chondrocytes to stimulate MMP production. The chondrocytes were incubated with CS‑IL‑1Ra microspheres to assess its effects on IL‑1β‑induced MMP expression. Chondrocyte proliferation and glycosaminoglycan (GAG) content were also determined. The mRNA expression and protein levels of IL‑1β, MMP‑1, MMP‑3 and MMP‑13 were detected using reverse transcription‑polymerase chain reaction and western blotting analyses, respectively. The IL‑1Ra release kinetics were characterized by an initial burst release, which was reduced to a linear release over seven days. The mRNA expression levels and protein levels of IL‑1β, MMP‑1, MMP‑3 and MMP‑13 were reduced compared with the control group. The present study demonstrated the chondroprotective properties of CS microspheres as a controlled release system carrying IL‑1Ra, due to the ability of the system to downregulate the expression of osteoarthritis‑associated matrix‑degrading proteinases in chondrocytes.
View Figures
View References

Related Articles

Journal Cover

January-2015
Volume 11 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhou PH, Ma BL, Shi L, Xie T and Qiu B: Inhibition of interleukin‑1β‑stimulated matrix metalloproteinases via the controlled release of interleukin‑1Ra from chitosan microspheres in chondrocytes. Mol Med Rep 11: 555-560, 2015
APA
Zhou, P., Ma, B., Shi, L., Xie, T., & Qiu, B. (2015). Inhibition of interleukin‑1β‑stimulated matrix metalloproteinases via the controlled release of interleukin‑1Ra from chitosan microspheres in chondrocytes. Molecular Medicine Reports, 11, 555-560. https://doi.org/10.3892/mmr.2014.2743
MLA
Zhou, P., Ma, B., Shi, L., Xie, T., Qiu, B."Inhibition of interleukin‑1β‑stimulated matrix metalloproteinases via the controlled release of interleukin‑1Ra from chitosan microspheres in chondrocytes". Molecular Medicine Reports 11.1 (2015): 555-560.
Chicago
Zhou, P., Ma, B., Shi, L., Xie, T., Qiu, B."Inhibition of interleukin‑1β‑stimulated matrix metalloproteinases via the controlled release of interleukin‑1Ra from chitosan microspheres in chondrocytes". Molecular Medicine Reports 11, no. 1 (2015): 555-560. https://doi.org/10.3892/mmr.2014.2743