Exosomes from breast cancer cells stimulate proliferation and inhibit apoptosis of CD133+ cancer cells in vitro

Shi,Jianhua; Ren,Yi; Zhen,Linlin; Qiu,Xiaolan

doi:10.3892/mmr.2014.2749

January-2015 Volume 11 Issue 1

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

January-2015 Volume 11 Issue 1

Full Size Image

Article

Exosomes from breast cancer cells stimulate proliferation and inhibit apoptosis of CD133+ cancer cells in vitro

Authors:
- Jianhua Shi
- Yi Ren
- Linlin Zhen
- Xiaolan Qiu
View Affiliations / Copyright

Affiliations: Department of Breast and Thyroid Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223001, P.R. China
Pages: 405-409
|
Published online on: October 22, 2014

https://doi.org/10.3892/mmr.2014.2749
Expand metrics +

Abstract

Exosomes are a family of bioactive vesicles that are secreted from various types of cell, including tumor cells. Exosomes derived from breast cancer cells have been demonstrated to perform important functions in tumor progression in vitro and in vivo. However, few studies exist regarding the function of exosomes in CD133+ breast cancer cells. In the present study, exosomes from 4T1 mouse breast cancer cells and mouse mammary gland epithelial cells were purified. The exosome‑associated markers CD63, CD9 and CD81 were detected, and the size distribution and ζ potential of the exosomes were determined. Exosome uptake by CD133+ and CD133‑ 4T1 cells was confirmed by confocal microscopy. An ATPlite assay indicated that the proliferation of CD133+ cells was increased and the apoptosis was suppressed by exosomes from 4T1 cells. Collectively, the findings of the present study demonstrate a novel mechanism by which the action of exosomes on CD133+ 4T1 cells may contribute to breast cancer progression.

View Figures

View References

1	Fan W, Tian XD, Huang E and Zhang JJ: Exosomes from CIITA-transfected CT26 cells enhance anti-tumor effects. Asian Pac J Cancer Prev. 14:987–991. 2013. View Article : Google Scholar : PubMed/NCBI
2	Wolfers J, Lozier A, Raposo G, et al: Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming. Nat Med. 7:297–303. 2001.PubMed/NCBI
3	Clayton A, Mitchell JP, Court J, et al: Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2. Cancer Res. 67:7458–7466. 2007. View Article : Google Scholar : PubMed/NCBI
4	Hartman ZC, Wei J, Glass OK, et al: Increasing vaccine potency through exosome antigen targeting. Vaccine. 29:9361–9367. 2011. View Article : Google Scholar : PubMed/NCBI
5	Clayton A, Al-Taei S, Webber J, et al: Cancer exosomes express CD39 and CD73, which suppress T cells through adenosine production. J Immunol. 187:676–683. 2011. View Article : Google Scholar : PubMed/NCBI
6	Cai Z, Yang F, Yu L, et al: Activated T cell exosomes promote tumor invasion via Fas signaling pathway. J Immunol. 188:5954–5961. 2012. View Article : Google Scholar : PubMed/NCBI
7	Munich S, Sobo-Vujanovic A, Buchser WJ, et al: Dendritic cell exosomes directly kill tumor cells and activate natural killer cells via TNF superfamily ligands. Oncoimmunology. 1:1074–1083. 2012. View Article : Google Scholar : PubMed/NCBI
8	Webber J, Steadman R, Mason MD, et al: Cancer exosomes trigger fibroblast to myofibroblast differentiation. Cancer Res. 70:9621–9630. 2010. View Article : Google Scholar : PubMed/NCBI
9	Bonito MD, Cantile M, Malzone G, et al: The prognostic role of cancer stem cells in breast tumors. J Clin Med Res. 5:325–326. 2013.PubMed/NCBI
10	Wang Q, Zhuang X, Mu J, et al: Delivery of therapeutic agents by nanoparticles made of grapefruit-derived lipids. Nat Commun. 4:18672013. View Article : Google Scholar : PubMed/NCBI
11	Yang C and Robbins PD: The roles of tumor-derived exosomes in cancer pathogenesis. Clin Dev Immunol. 2011:8428492011. View Article : Google Scholar : PubMed/NCBI
12	Yang C and Robbins PD: Immunosuppressive exosomes: a new approach for treating arthritis. Int J Rheumatol. 2012:5735282012. View Article : Google Scholar : PubMed/NCBI
13	Stoeck A, Keller S, Riedle S, et al: A role for exosomes in the constitutive and stimulus-induced ectodomain cleavage of L1 and CD44. Biochem J. 393:609–618. 2006. View Article : Google Scholar : PubMed/NCBI
14	Zomer A, Vendrig T, Hopmans ES, et al: Exosomes: Fit to deliver small RNA. Commun Integr Biol. 3:447–450. 2010. View Article : Google Scholar : PubMed/NCBI
15	Andreola G, Rivoltini L, Castelli C, et al: Induction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles. J Exp Med. 195:1303–1316. 2002. View Article : Google Scholar : PubMed/NCBI
16	Huber V, Fais S, Iero M, et al: Human colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape. Gastroenterology. 128:1796–1804. 2005. View Article : Google Scholar : PubMed/NCBI
17	Keryer-Bibens C, Pioche-Durieu C, Villemant C, et al: Exosomes released by EBV-infected nasopharyngeal carcinoma cells convey the viral latent membrane protein 1 and the immunomodulatory protein galectin 9. BMC Cancer. 6:2832006. View Article : Google Scholar
18	Liu ZM, Wang YB and Yuan XH: Exosomes from murine-derived GL26 cells promote glioblastoma tumor growth by reducing number and function of CD8⁺ T cells. Asian Pac J Cancer Prev. 14:309–314. 2013. View Article : Google Scholar : PubMed/NCBI
19	Cai Z, Zhang W, Yang F, et al: Immunosuppressive exosomes from TGF-β1 gene modified dendritic cells attenuate Th17-mediated inflammatory autoimmune disease by inducing regulatory T cells. Cell Res. 22:607–610. 2012.
20	Peinado H, Lavotshkin S and Lyden D: The secreted factors responsible for pre-metastatic niche formation: old sayings and new thoughts. Semin Cancer Biol. 21:139–146. 2011. View Article : Google Scholar : PubMed/NCBI
21	Channavajjhala SK, Rossato M, Morandini F, et al: Optimizing the purification and analysis of miRNAs from urinary exosomes. Clin Chem Lab Med. 52:345–354. 2014. View Article : Google Scholar : PubMed/NCBI
22	Cho JA, Park H, Lim EH, et al: Exosomes from ovarian cancer cells induce adipose tissue-derived mesenchymal stem cells to acquire the physical and functional characteristics of tumor-supporting myofibroblasts. Gynecol Oncol. 123:379–386. 2011. View Article : Google Scholar