Open Access

Resveratrol attenuates intermittent hypoxia‑induced insulin resistance in rats: Involvement of Sirtuin 1 and the phosphatidylinositol‑4,5‑bisphosphate 3‑kinase/AKT pathway

  • Authors:
    • Qiuyan Wang
    • Xiaohui Sun
    • Xiaobin Li
    • Xiang Dong
    • Peng Li
    • Li Zhao
  • View Affiliations

  • Published online on: October 23, 2014     https://doi.org/10.3892/mmr.2014.2762
  • Pages: 151-158
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Obstructive sleep apnea can induce chronic intermittent hypoxia (CIH) during sleep and is associated with obesity and diabetes. Resveratrol (RSV), a polyphenolic phytoalexin, can regulate glucose metabolism, thereby reducing insulin resistance. The present study aimed to assess whether RSV attenuates CIH‑induced insulin resistance in rats and the underlying mechanisms. A total of 40 rats were randomly assigned into five groups: i) Control; ii) subjected to CIH only; iii) subjected to CIH and treated with 3 mg/kg/day of RSV; iv) subjected to CIH and treated with 30 mg/kg/day of RSV; v) subjected to CIH and treated with 60 mg/kg/day of RSV. All animals were sacrificed following 28 days of treatment. Subsequently, the blood and livers were harvested and blood insulin and glucose levels were measured. Levels of sirtuin (Sirt) 1, insulin receptor (InsR) and glucose transporter 2 (Glut2) in the liver were measured. RSV treatment was demonstrated to suppress weight gain and improve hepatic morphology. RSV treatment also significantly reduced the homeostasis model assessment estimate of insulin resistance of the rats exposed to CIH. This effect occurred in a dose‑dependent manner. RSV significantly upregulated liver Sirt1 levels and inhibited InsR and Glut2 expression in the liver. Additionally, RSV activated the phosphorylation of phosphatidylinositol‑4,5‑bisphosphate 3‑kinase (PI3K) and AKT. The present study demonstrates that RSV prevents CIH‑induced insulin resistance in rats. Upregulation of Sirt1 and activation of PI3K/AKT signaling may be involved in this process.
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Spandidos Publications style
Wang Q, Sun X, Li X, Dong X, Li P and Zhao L: Resveratrol attenuates intermittent hypoxia‑induced insulin resistance in rats: Involvement of Sirtuin 1 and the phosphatidylinositol‑4,5‑bisphosphate 3‑kinase/AKT pathway. Mol Med Rep 11: 151-158, 2015
APA
Wang, Q., Sun, X., Li, X., Dong, X., Li, P., & Zhao, L. (2015). Resveratrol attenuates intermittent hypoxia‑induced insulin resistance in rats: Involvement of Sirtuin 1 and the phosphatidylinositol‑4,5‑bisphosphate 3‑kinase/AKT pathway. Molecular Medicine Reports, 11, 151-158. https://doi.org/10.3892/mmr.2014.2762
MLA
Wang, Q., Sun, X., Li, X., Dong, X., Li, P., Zhao, L."Resveratrol attenuates intermittent hypoxia‑induced insulin resistance in rats: Involvement of Sirtuin 1 and the phosphatidylinositol‑4,5‑bisphosphate 3‑kinase/AKT pathway". Molecular Medicine Reports 11.1 (2015): 151-158.
Chicago
Wang, Q., Sun, X., Li, X., Dong, X., Li, P., Zhao, L."Resveratrol attenuates intermittent hypoxia‑induced insulin resistance in rats: Involvement of Sirtuin 1 and the phosphatidylinositol‑4,5‑bisphosphate 3‑kinase/AKT pathway". Molecular Medicine Reports 11, no. 1 (2015): 151-158. https://doi.org/10.3892/mmr.2014.2762