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Article Open Access

Expression analysis of the estrogen receptor target genes in renal cell carcinoma

  • Authors:
    • Zhihong Liu
    • You Lu
    • Zonghai He
    • Libo Chen
    • Yiping Lu
  • View Affiliations / Copyright

    Affiliations: Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 75-82
    |
    Published online on: October 24, 2014
       https://doi.org/10.3892/mmr.2014.2766
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Abstract

The aim of the present study was to investigate the differentially expressed genes (DEGs) and target genes of the estrogen receptor (ER) in renal cell carcinoma. The data (GSE12090) were downloaded from the gene expression omnibus database. Data underwent preprocessing using the affy package for Bioconductor software, then the DEGs were selected via the significance analysis of microarray algorithm within the siggenes package. Subsequently, the DEGs underwent functional and pathway enrichment analysis using Database for Annotation Visualization and Integrated Discovery software. Following data analysis, transcriptional regulatory networks between the DEGs and transcription factors were constructed. Finally, the ER target genes were subjected to gene ontology enrichment analysis. A total of 215 DEGs were identified between the chromophobe renal cell carcinoma samples and the oncocytoma samples, including 126 upregulated and 89 downregulated genes. Functional enrichment analysis indicated that 25% of the DEGs were significantly enriched in functions associated with the plasma membrane. Among those DEGs, 105 were regulated by the ER. Further regulatory network analysis indicated that the ER was mainly involved in the regulation of oncogenes and tumor suppressor genes, including protease serine 8, claudin 7 and Ras‑related protein Rab‑25. In the present study, the identified ER target genes were demonstrated to be closely associated with tumor development; this knowledge may improve the understanding of the ER regulatory mechanisms during tumor development and promote the discovery of predictive markers for renal cell carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Z, Lu Y, He Z, Chen L and Lu Y: Expression analysis of the estrogen receptor target genes in renal cell carcinoma. Mol Med Rep 11: 75-82, 2015.
APA
Liu, Z., Lu, Y., He, Z., Chen, L., & Lu, Y. (2015). Expression analysis of the estrogen receptor target genes in renal cell carcinoma. Molecular Medicine Reports, 11, 75-82. https://doi.org/10.3892/mmr.2014.2766
MLA
Liu, Z., Lu, Y., He, Z., Chen, L., Lu, Y."Expression analysis of the estrogen receptor target genes in renal cell carcinoma". Molecular Medicine Reports 11.1 (2015): 75-82.
Chicago
Liu, Z., Lu, Y., He, Z., Chen, L., Lu, Y."Expression analysis of the estrogen receptor target genes in renal cell carcinoma". Molecular Medicine Reports 11, no. 1 (2015): 75-82. https://doi.org/10.3892/mmr.2014.2766
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Lu Y, He Z, Chen L and Lu Y: Expression analysis of the estrogen receptor target genes in renal cell carcinoma. Mol Med Rep 11: 75-82, 2015.
APA
Liu, Z., Lu, Y., He, Z., Chen, L., & Lu, Y. (2015). Expression analysis of the estrogen receptor target genes in renal cell carcinoma. Molecular Medicine Reports, 11, 75-82. https://doi.org/10.3892/mmr.2014.2766
MLA
Liu, Z., Lu, Y., He, Z., Chen, L., Lu, Y."Expression analysis of the estrogen receptor target genes in renal cell carcinoma". Molecular Medicine Reports 11.1 (2015): 75-82.
Chicago
Liu, Z., Lu, Y., He, Z., Chen, L., Lu, Y."Expression analysis of the estrogen receptor target genes in renal cell carcinoma". Molecular Medicine Reports 11, no. 1 (2015): 75-82. https://doi.org/10.3892/mmr.2014.2766
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